题名 |
良性攝護腺肥大(Benign Prostatic Hyperplasia, BPH)生物性病因的探討 |
并列篇名 |
A Study on the Bioetiology of Benign Prostatic Hyperplasia, BPH |
DOI |
10.29540/CNABST.201012.0009 |
作者 |
陳椒華(Jiau-Hua Chen);王美苓(Mei-Ling Wang);王淑珍(Shu-Jen Wang);鄭哲舟(Tse-Chou Cheng) |
关键词 |
良性攝護腺肥大 ; 生物性病因 ; 核酸聚合酶連鎖反應 ; Benign prostatic hyperplasia BPH ; Bioetiology ; Polymerase chain reaction PCR |
期刊名称 |
嘉南學報(科技類) |
卷期/出版年月 |
36期(2010 / 12 / 01) |
页次 |
65 - 71 |
内容语文 |
繁體中文 |
中文摘要 |
本研究利用核酸聚合酶連鎖反應技術(polymerase chain rcaction, PC),針對男性普遍發生的攝護腺組織增生疾病,良性攝護腺肥大症(Benign prostatic hyperplasia, BPH)組織進行生物性病因檢測研究,選擇各種可能存在於良性攝護腺肥大組織的細菌性(Echerichia, Mycoplasma, Ureaplasma)或病毒性生物因子之部份核酸序列做爲PCR引子,再選定最適反應條件並進行PCR反應,經檢測良性攝護腺肥大76個組織檢體,其中49個檢體檢測出含有E. coli 23S DNA序列,但並未檢測出任何,Mycoplasma及Ureaplasma生物因子存在。本實驗也利用商業Takara Mycoplasma, Human Cytomegalovirus及Human Papillomavirus detection kit來進行檢測檢體,在所檢測35個檢體,也沒有檢測出Mycoplasma,Human Cytomegalovirus及Human Papillomavirus。 |
英文摘要 |
In this study, polymerase chain reaction (PCR) was used to investigate the bioetiology of benign prostatic hyperplasia (BPH), a common disease in men. Specific nucleotide sequences of Escherichia, Mycopiasma Ureapiasma and some potentially pathogenic viruses were selected as PCR detection primers. Seventy-six prostate specimens of BPH were properly prepared for PCR test. Part of DNA sequences of E. coil 23S was found in 49 specimens, and no nucleodites of Mycopiasma and Ureaplasma were detected in all 76 specimens. Takara Kit was also used in this study to detect the nucleotides of Mycopiasma, Human Cytomegalovirus, and Human Papillomavirus. No evidence of existence of these three microorganisms was found in 35 specimens being tested with this commercial kit for PCR test. |
主题分类 |
基礎與應用科學 >
基礎與應用科學綜合 基礎與應用科學 > 化學 醫藥衛生 > 醫藥衛生綜合 醫藥衛生 > 藥理醫學 |