球孢白殭菌（Beauveria bassiana Bals.）CCC 801菌株感染甘藍害蟲之致病力及其雙相培養特性探討

Studies on the Pathogenicity Against the Pests on Cabbage and Diphasic Cultivation Characteristics of Beauveria bassiana Bals. CCC 801

張方宜(Fang-I Chang)；莊益源(Yi-Yuan Chuang)；陳啟予(Chi-Yu Chen)

球孢白殭菌 ； 甘藍害蟲 ； 芽生孢子 ； 雙相培養 ； Beauveria bassiana ； Pests on cabbage ； Blastospore ； Diphasic cultivation

臺東區農業改良場研究彙報

34輯（2024 / 06 / 01）

29 - 45

繁體中文;英文

本試驗採集自田間罹病之咖啡果小蠹（Hypothenemus hampei（Ferrari））並分離出球孢白殭菌（Beauveria bassiana Bals.）CCC 801，將其孢子懸浮液濃度配製成10^7 conidia/ml，於室內25°C及高相對濕度環境下進行對黃條葉蚤（Phyllotreta striolata（Fabricius））、小菜蛾（Plutella xylostella（Linnaeus））及偽菜蚜（Lipaphis erysimi（Kaltenbach））等十字花科蔬菜重要害蟲之致病力測試，結果顯示接種孢子懸浮液7天後，感染率皆可達77%以上，顯示此菌株具有寄主範圍廣泛之特性。將CCC 801菌株以六種不同穀類基質進行固態培養試驗，結果顯示該菌株在白米基質上培養至第14天的產孢速率最快且產孢量最多，可達1.9x 10^8 conidia/g。於暗環境下利用四種液態培養基震盪搖瓶進行CCC 801菌株之液態培養試驗，結果顯示培養7天後，其於SMAY培養基中產生的芽生孢子量最高，可達1.8x10^8 blastospore/ml。再將上述經液態培養7天後之四種液態培養基產生的CCC 801芽生孢子懸浮液稀釋成10^7 blastospore/ml，接種於白米基質上進行雙相培養試驗，結果顯示培養14天後，以SMAY之芽生孢子懸浮液接種的產孢量最高，可達8.2x10^8 conidia/g。白殭菌CCC 801菌株對多種十字花科蔬菜害蟲具優異的感染效果，且室內初步量產之雙相培養結果，可供做往後菌株量產及田間應用之參考。

In this experiment, Beauveria bassiana Bals. CCC 801 was isolated from the diseased coffee beetle (Hypothenemus hampei (Ferrari)). The pathogenicity of the conidial suspension of CCC 801 (10^7 conidia/ml) as the inoculum under high relative humidity in the laboratory (25°C) against other pests such as Phyllotreta striolata (Fabricius), Plutella xylostella (Linnaeus) and Lipaphis erysimi (Kaltenbach) on cabbage was conducted. The results showed that the average mortality was above 77% at 7 days after inoculation, indicating that CCC 801 possess broad-host pathogenicity. Six different kinds of grains were used for mass spore production of CCC 801 by solid cultivation. The results showed that the strain had the fastest sporulation rate and the largest spore production at 14 days after cultivation by rice substrate, which could reach 1.9x10^8 conidia/g. In addition, CCC 801 liquid cultivation was performed in a dark environment using four kinds of liquid medium with rotatory shaking. The results showed that the highest blastospore concentration of CCC 801 reached 1.8x10^8 blastospore/ml for SMAY liquid medium at 7 days after cultivation. Besides, the CCC 801 blastospore suspensions produced by the above four liquid medium after 7 days cultivation were diluted to 10^7 blastospore/ml and inoculated on the rice substrate for diphasic cultivation test. The results showed that the highest spore concentration of CCC 801 reached 8.2x10^8 conidia/g inoculated with SMAY blastospore suspension at 14 days after cultivation. This study suggests that B. bassiana CCC 801 strain possesses excellent infective effects on a variety of vegetable pests, and the diphasic cultivation results of CCC 801 in the laboratory can be used for mass spore production and held application of them in the future.