耐力運動訓練對誘發骨骼肌細胞凋亡之粒腺體訊息傳遞路徑的影響

The Effect of Endurance Exercise Training on the Mitochondria Signaling Pathway of Apoptosis in Skeletal Muscle

10.6222/pej.3904.200612.1402

陳聰毅(Tsung-I Chen)；林念璁(Wu-Chu Chen)

細胞凋亡 ； 粒腺體 ； 運動訓練 ； 骨骼肌 ； apoptosis ； mitochondrial ； exercise ； skeletal muscle

體育學報

39卷4期（2006 / 12 / 01）

11 - 22

繁體中文

Purpose: This study is to investigate the influence of exercise training on skeletal muscle apoptosis induced by mitochondrial pathway. Methods: Animals, male Wistar rats, were randomly assigned to either a control group or an exercise group. Exercise group were trained for 5 days weekly for 12 weeks on treadmill. An exhausted exercise test was performed after a rest period of 7 days after 12 weeks exercise training. Rats were sacrificed at pre-exercise (PRE), post-exercise 0 hour (PEO) and post-exercise 48 hour (PE48), respectively. The soleus and gastrocnemius were dissected out and frozen in liquid nitrogen rapidly. Results: The citrate synthesis activity of soleus of trained was significantly higher than untrained at PRE, PE0 and PE48 (p＜.05), respectively. Both the muscle weight-to-body weight ratio of gastrocnemius and soleus of trained were significantly higher than untrained (p＜.05). In Bcl-2 expression, both of the gastrocnemius and soleus of trained were significantly higher than untrained at PE0 (p＜.05) and significantly lower than untrained at PE48 (p＜.05). In Bax expression, both of the gastrocnemius and soleus of trained were significantly lower than untrained at PE0 and PE48 (p＜.05), respectively. Both the apoptosis index of gastrocnemius and soleus of trained were significantly lower than untrained after PE48 (p＜.05). Conclusion: These findings suggest that skeletal muscle may modulate their Bcl-2 and Bax apoptotic signals expression and the oxidative activity of mitochondria after exercise training, lead to attenuating the time of skeletal muscle apoptosis or inhibiting the apoptotic signals expression, and then the probability of hypertrophy occurred.

Purpose: This study is to investigate the influence of exercise training on skeletal muscle apoptosis induced by mitochondrial pathway. Methods: Animals, male Wistar rats, were randomly assigned to either a control group or an exercise group. Exercise group were trained for 5 days weekly for 12 weeks on treadmill. An exhausted exercise test was performed after a rest period of 7 days after 12 weeks exercise training. Rats were sacrificed at pre-exercise (PRE), post-exercise 0 hour (PEO) and post-exercise 48 hour (PE48), respectively. The soleus and gastrocnemius were dissected out and frozen in liquid nitrogen rapidly. Results: The citrate synthesis activity of soleus of trained was significantly higher than untrained at PRE, PE0 and PE48 (p＜.05), respectively. Both the muscle weight-to-body weight ratio of gastrocnemius and soleus of trained were significantly higher than untrained (p＜.05). In Bcl-2 expression, both of the gastrocnemius and soleus of trained were significantly higher than untrained at PE0 (p＜.05) and significantly lower than untrained at PE48 (p＜.05). In Bax expression, both of the gastrocnemius and soleus of trained were significantly lower than untrained at PE0 and PE48 (p＜.05), respectively. Both the apoptosis index of gastrocnemius and soleus of trained were significantly lower than untrained after PE48 (p＜.05). Conclusion: These findings suggest that skeletal muscle may modulate their Bcl-2 and Bax apoptotic signals expression and the oxidative activity of mitochondria after exercise training, lead to attenuating the time of skeletal muscle apoptosis or inhibiting the apoptotic signals expression, and then the probability of hypertrophy occurred.

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1. 陳聰毅、林念璁(2010)。耐力訓練對骨骼肌肌纖維類型與細胞凋亡關係的影響。體育學報，43(3)，17-34。