運動後碳水化合物補充對人體骨骼肌代謝基因表現效應－寡核糖核酸微陣列分析

Effect of post-exercise Carbohydrate Ingestion on Metabolic Gene Expression in Human Skeletal Muscle an Oligonucleotide Microarray Analysis

10.6222/pej.4301.201003.1503

廖淑芬(Su-Fen Liao)；林一郎(E-Long Lin)；巫錦霖(Chin-Lin Wu)；程一雄(I-Shiung Cheng)

運動 ； 骨骼肌肉 ； 寡核糖核酸微陣列 ； exercise ； skeletal muscle ； oligonucleotide microarray

體育學報

43卷1期（2010 / 03 / 01）

13 - 23

繁體中文

目的：這次研究想了解運動後碳水化合物補充對人體骨骼肌代謝相關基因表現改變的效果。方法：六名受試者完成二種試驗處理，在完成單一次60分鐘70-75%攝氧量峰值(CO2 peak)運動後，分別禁食或補充碳水化合物，運動後第3小時，進行肌肉穿刺，肌肉樣本貯存於液態氮，待寡核糖核酸微陣列分析。結果：和運動後禁食比較，運動後碳水化合物補充後3小時人體骨骼肌肉基因表現，共有98個基因改變，其中57個基因增加表現，41個法因降低表現。結論：運動恢復期碳水化合物的補充，的確明顯改變人體骨骼肌肉代謝基因表現。

Purpose: The purpose of this study was to understand the effect of carbohydrate ingestion on metabolic gene expressions in human skeletal muscle during post-exercise recovery. Methods: Six subjects participated in two experimental trials. In one of the experimental trials, they ingested a high carbohydrate mixed meal immediately after a cycle ergometer for 60 minutes at 70-75% VO2 peak. In the other one, they were not allowed to eat for 3 hours after the same exercise protocol. The muscle samples of these subjects were obtained from vastus lateralis with a needle biopsy technique 3 hours later after exercise. These muscle samples were analyzed using the oligonucleotide microarray. Results: The result showed that the muscle samples from those who took a carbohydrate mixed meal had 57 genes up-regulated and 41 genes down-regulated compared to the samples from the fasting condition. Conclusion: The ingestion of a high carbohydrate mixed meal during post-exercise recovery was suggested to alter metabolic gene expressions in human skeletal muscle.

1. 程一雄、廖淑芬、巫錦霖、李寧遠(2007)。運動後不同升糖指數碳水化合物對人餿骨骼葡萄糖轉運子影響。大專體育學刊，9(1)，115-126。
   連結：
2. Aoi, W.,Ichiishi, E.,Sakamoto, N.,Tsujimoto, A.,Tokuda, H.,Yoshikawa, T.(2004).Effect of exercise on hepatic gene expression in rats: A microarray analysis.Life Science,75,3117-3128.
3. Balon, T. W.,Yerneni, K. K. V.(2001).Redox regulation of skeletal muscle glucose transport.Medicine and Science in Sports and Exercise,3,382-385.
4. Bergstrom, J.(1962).Muscle electrolytes in man.The Scandinavian Journal of Clinical & Laboratory Investigation Supplementum,68,1-100.
5. Bergström, J.,Hultman, E.(1966).Muscle glycogen synthesis after exercise: An enhancing factor localized to the muscle cell in man.Nature,210,309-310.
6. Cameron-Smith, D.(2002).Exercise and skeletal muscle gene expression.Clinical and Experimental Pharmacology & Physiology,29,309-213.
7. Cameron-Smith, D.,Burke, L. M.,Angus, D. J.,Junstall, R. J.,Cox, G. R.,Bonen, A.(2003).A short-term, high-fat diet up-regulates lipid metabolism and gene expression in human skeletal muscle.The American Journal of Clinical Nutrition,77,313-318.
8. Cheng, I. S.,Lee, N. Y.,Liu, K. L.,Liao, S. F.,Huang, C. H.,Kuo, C. H.(2005).Effect of postexercise carbohydrate supplementation on glucose uptake-associated geneexpression in human skeletal muscle.The Journal of Nutritional Biochemistry,16,267-271.
9. Diffee, G. M.,Seversen, E. A.,Stein, T. D.,Johnson, J. A.(2003).Microarray expression analysis of effects of exercise training increase in atrial MLC-1 in rat ventricles.American journal of Physiology: Heart and Circulatory Physiology,284,830-837.
10. Downward, J.(1995).Signal transduction. A target for PI (3) kinase.Nature,376,553-554.
11. Fukai, T.,Siegfried, M. R.,Ushio-Fukai, M.,Cheng, Y.,Kojda, G.,Harrison. D. G.(2000).Regulation of the vascular extracellular superoxide dismutase by nitric oxide and exercise training.The journal of Clinical Investigation,105,1631-1639.
12. Lien, H. C.,Hsiao, Y. H.,Lin, Y. S.,Yao, Y. T.,Juan, H. F.,Kuo, W. H.(2007).Molecular signatures of metaplastic carcinoma of the breast by large-scale transcriptional profiling: Identification of genes potentially related to epithelialmesenchymal transition.Oncogene,26,1-13.
13. Majer, M.,Popov, K. M.,Harris, R. A.,Bogardus, C.,Drochazka, M.(1998).Insulin downregulates pyruvate dehydrogenase kinase (PDK) mRNA: Potential mechanism contributing to increased lipid oxidation in insulin-resistant subjects.Molecular Genetics and Metabolism,65,181-186.
14. Nielsen, J. N.,Frosig, C.,Sajan, M. P.,Miura, A.,Standaert, M. L.,Graham, B. A.(2003).Increased atypical PKC activity in endurance-trained human skeletal muscle.Biochemical and Biophysical Reseatch Communications,312,1147-1153.
15. Pilegaard, H.,Ordway, G. A.,Saltin, B.,Neufer, P. B.(2000).Transcriptional regulation of gene expression in human skeletal muscle during recovery from exercise.American Journal of Physiology: Endocrinology, Metabolism and Gastrointestinal Physiology,279,806-814.
16. Savkur, R. S.,ByamLett, K. S.,Michael, L. F.,Burns, T. P.(2005).Regulation of pyruvate dehydrogenase kinase expression by the furnesoid X receptor.Biochemical and Biophysical Research Communications,329,391-396.
17. Sugden, M. C.,Bulmer, K.,Gibbons, G. F.,Holness, M. J.(2001).Role of peroxisome proliferators-activated receptor-cc in the mechanism underlying changes in renal pyruvate dehydrogenase kinase isoform 4 protein expression in starvation and after refeeding.Archives of Biochemistry and Biophysics,395,246-252.
18. Tong, L.,Shen, H.,Perreau, V. M.,Balazs, R.,Cotman, C. W.(2001).Effects of exercise on gene-expression profile in the rat hippocampus.Neurobiology of Disease,8,62-66.
19. Wittwer, M.,Billeter, R.,Hoppeler, H.,Fluck, M.(2004).Regulatory gene expression in skeletal muscle of highly endurance-trained humans.Acta Physiologica Scandinavica,180,217-227.
20. Wu, C. L.,Nicolas, C.,Williams, C.,Took, A.,Hardy, L.(2003).The influence of high-carbohydrate meals with different glycaemic indices on substrate utilization during subsequent exercise.The British Journal of Nutrition,90,1049-1056.
21. Zorzano, A.,Palacin, M.,Gums, A.(2005).Mechanisms regulating GLUT4 glucose transpoter expression and glucose transpoter in skeletal muscle.Acta Physiologica Scandinavica,183,43-58.
22. 林正常(1994)。運動生理學實驗指導。臺北市:師大書苑。
23. 程一雄、廖淑芬、劉凱莉、鄭敏琳、陳志輝、林一郎、王昭文、李寧遠(2005)。運動後全穀類飲食對血液生化值和骨骼肌葡萄糖轉運子基因表現影響。中華營誌，30(2)，81-89。