單次離心運動對骨骼肌粒腺體釋出細胞色素C及細胞凋亡誘發因子的影響

The Effect of Single Bout Eccentric Exercise on Mitochondria Released Cytochrome C and Apoptosis Inducing Factor in Skeletal Muscle

10.6222/pej.4104.200812.0802

陳聰毅(Tsung-I Chen)；林念璁(Nien-Tsung Lin)

離心運動 ； 粒腺體 ； 細胞色素c ； 細胞凋亡誘發因子 ； eccentric exercise ； mitochondria ； cytochrome c ； apoptosis inducing factor

體育學報

41卷4期（2008 / 12 / 01）

15 - 28

繁體中文

Purpose: This study was to investigate the effect of eccentric exercise on mitochondria to release the cytochrome c (cyt-c) and apoptosis inducing factor (AIF) in skeletal muscle. Methods: Animals, male Sprague-Dawley rats, were randomly assigned to either a rest control group (N=4), an exercise control group (level running, LR; N=16) or an eccentric exercise group (downhill running, DHR; N=16). The rats in both LR and DHR ran on animal treadmill at a constant speed of 20 m/min on the level (0 degree incline) or downhill (a 16 degree decline) for 90 min. The 90 min running time was divided into 5 min runs separated by 2 min rest intervals (18 bouts). Rats were sacrificed at post-exercise 0 hour (PE0; n=4), post-exercise 24 hours (PE24; n=4), post-exercise 48 hours (PE48; n=4) and post-exercise 72 hours (PE72; n=4), respectively. The triceps brachii (TB) muscle was dissected out and frozen in liquid nitrogen rapidly. Results: The phosphorylated c-Jun NH2-terminal kinase (p-JNK) expression of DHR was significantly higher than LR at PE0 (p＜.05). The expression level of cytosolic cyt-c released from mitochondria of DHR which was significantly higher than LR at both PE24 and PE48 (p ＜ .05). The expression level of nuclei AIF of DHR was significantly higher than LR at PE24 (p ＜ .05). Conclusions: 1) A single bout eccentric exercise might increase the expression level of cytosolic cyt-c released from mitochondria in TB at both PE24 and PE48. 2) A single bout eccentric exercise didn't affect the expression level of cytosolic AIF released from mitochondria in TB. However, the expression level of nuclei AIF was increased at PE24 after a single bout eccentric exercise.

Purpose: This study was to investigate the effect of eccentric exercise on mitochondria to release the cytochrome c (cyt-c) and apoptosis inducing factor (AIF) in skeletal muscle. Methods: Animals, male Sprague-Dawley rats, were randomly assigned to either a rest control group (N=4), an exercise control group (level running, LR; N=16) or an eccentric exercise group (downhill running, DHR; N=16). The rats in both LR and DHR ran on animal treadmill at a constant speed of 20 m/min on the level (0 degree incline) or downhill (a 16 degree decline) for 90 min. The 90 min running time was divided into 5 min runs separated by 2 min rest intervals (18 bouts). Rats were sacrificed at post-exercise 0 hour (PE0; n=4), post-exercise 24 hours (PE24; n=4), post-exercise 48 hours (PE48; n=4) and post-exercise 72 hours (PE72; n=4), respectively. The triceps brachii (TB) muscle was dissected out and frozen in liquid nitrogen rapidly. Results: The phosphorylated c-Jun NH2-terminal kinase (p-JNK) expression of DHR was significantly higher than LR at PE0 (p＜.05). The expression level of cytosolic cyt-c released from mitochondria of DHR which was significantly higher than LR at both PE24 and PE48 (p ＜ .05). The expression level of nuclei AIF of DHR was significantly higher than LR at PE24 (p ＜ .05). Conclusions: 1) A single bout eccentric exercise might increase the expression level of cytosolic cyt-c released from mitochondria in TB at both PE24 and PE48. 2) A single bout eccentric exercise didn't affect the expression level of cytosolic AIF released from mitochondria in TB. However, the expression level of nuclei AIF was increased at PE24 after a single bout eccentric exercise.

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1. 陳聰毅、林念璁(2010)。耐力訓練對骨骼肌肌纖維類型與細胞凋亡關係的影響。體育學報，43(3)，17-34。