Timing effects of caffeine ingestion on acute testosterone and cortisol responses to resistance exercise

咖啡因攝取時機對阻力運動後睪固酮與皮質固醇反應之影響

10.3966/10247297201903520S003

吳柏翰(Bo-Han Wu)

ergogenic aids ； nutritional supplementation ； resistance exercise ； 增補劑 ； 營養增補 ； 阻力運動

體育學報

52卷S期（2019 / 03 / 01）

31 - 45

英文

Introduction: The purpose of this study was to examine the effects of different caffeine ingestion timing on hormonal responses immediate after resistance exercise (RE). Methods: Twelve university male students (age, 21.4 ± 1.9yr; mass, 70.1 ± 4.8kg) who regularly performed RE participate in this study. All subjects performed 1RM test, then assigned to four treatments: caffeine ingestion 60 min prior to RE (PRE60, 6mg.kg^(-1)), caffeine ingestion immediately prior to RE (PRE-EXE, 6mg.kg^(-1)), caffeine ingestion during RE (EXE, 6 mg.kg^(-1)) and control (CON) in counter balance order and within subject crossover design. All subjects ingested caffeine according to each treatment, then, the subjects performed RE (two exercises, 5 sets of 10 repetitions at 75% of 1RM). Blood samples were collected prior to caffeine ingestion (baseline, BL) and 0, 15, 30 min post to RE (P0, P15, P30) for analysis of testosterone, cortisol, blood lactic acid, glucose and free fatty acid. Each experiment separated by 7 days. In this study, statistical analysis of a two-way analysis of variance (treatment by time) with repeated measures was applied. Statistical significance set at α = .05. Results: After ingesting caffeine, the concentration of free fatty acid (P0) in PRE60 was significantly higher than CON (0.57 ± 0.09 vs. 0.41±0.10 mmol/L) (p＜.05). The responses of testosterone (P0, P15, P30) (PRE60: 676.75±145.82, 643.38±149.25 and 575.50±192.44; PRE-EXE: 654.25±121.23, 619.50±121.63 and 571.25±123.97 vs. CON: 584.75±136.19, 531.25±146.57 and 496.38±137.42ng/dl, p＜.05) and cortisol (P0, P15, P30) (PRE60: 23.31 ± 3.00, 22.02 ± 2.16 and 20.13 ± 2.78; PRE-EXE: 22.29 ± 2.84, 21.86 ± 2.23 and 20.3 ± 4.40 vs. CON: 18.40 ± 2.30, 17.92 ± 2.41 and 16.21 ± 2.10μg/dl, p＜.05) were significantly higher than CON in PRE60 and PRE-EXE. Conclusion: The results of this study indicated that caffeine ingestion prior to RE (PRE60 and PRE-EXE) will significant affect the acute hormonal responses. However, caffeine ingestion during RE might not significantly change acute responses of anabolic and catabolic hormones.

緒論：本研究主要目的在探討不同咖啡因攝取時機對於阻力運動後荷爾蒙反應之影響。方法：本研究以12名有阻力訓練習慣之男性為受試者（age，21.4±1.9yr；mass，70.1±4.8kg）。所有受試者在實驗前皆接受最大肌力（1RM）測驗，隨後以重複量數與對抗平衡次序分配進行運動前60分鐘攝取（PRE60，6mg／kg）、運動前立即攝取（PRE-EXE，6mg／kg）、運動中攝取（EXE，6mg／kg）與控制（CON）等4次實驗處理，每次實驗間隔7天，受試者按實驗處理攝取咖啡因，隨後進行阻力運動（5組；75％1RM強度進行10次反覆；2個動作；每組休息時間為2分鐘）。在安靜休息期（baseline，BL）與運動後0、15、30分鐘（P0，P15，P30）血液進行分析。本研究以相依樣本二因子（攝取時機×時間）變異數分析（ANOVA）咖啡因攝取時機對荷爾蒙的反應（睪固酮與皮質固醇）與能量代謝指標（血糖、血乳酸、血中游離脂肪酸）之間是否有顯著差異。顯著水準α訂為.05。結果：在咖啡因攝取後，PRE60的血清游離脂肪酸與CON有顯著差異（FFA：0.57±0.09vs.0.41±0.10mmol／L，p＜.05），而血清睪固酮（P0，P15，P30）（PRE60：676.75±145.82，643.38±149.25 and 575.50±192.44；PRE-EXE：654.25±121.23，619.50±121.63 and 573.25±125.01 vs. CON：584.75±136.19，531.25±146.57 and 496.38±137.42ng／dl，p＜.05）與皮質固醇（P0，P15，P30）（PRE60：23.31±3.00，22.02±2.16 and 20.13±2.78；PRE-EXE：22.29±2.84，21.86±2.23 and 20.3±4.40 vs. CON：18.40±2.30，17.92±2.41 and 16.21±2.10μg／dl，p＜.05）的反應則顯著高於CON。結論：本研究結果指出阻力運動前攝取咖啡因（PRE60 and PRE-EXE）會顯著影響阻力運動後立即之同化性與異化性荷爾蒙的反應，但是在運動中攝取則皆無顯著影響。

1. Ahtiainen, J. P,Pakarinen, A,Kraemer, W. J,Häkkinen, K.(2004).Acute hormonal responses to heavy resistance exercise in strength athletes versus nonathletes.Canadian Journal of Applied Physiology,29(5),527-543.
2. Astorino, T. A.,Rohmann, R. L.,Firth, K.(2008).Effect of caffeine ingestion on one-repetition maximal muscular strength.European Journal of Applied Physiology,102,127-132.
3. Baechle, T. R.,Earle, R. W.(2000).Essentials of Strength Training and Conditioning.Champaign, IL:Human Kinetics.
4. Beaven, C. M.,Hopkins, W. G.,Hansen, K. T.,Wood, M. R.,Cronin, J. B.,Lowe, T. E.(2008).Dose effect of caffeine on testosterone and cortisol responses to resistance exercise.International Journal of Sport Nutrition and Exercise Metabolism,18,131-141.
5. Beck, T. W.,Housh, T. J.,Schmidt, R. J.,Johnson, G. O.,Housh, D. J.,Coburn, J. W.,Malek, M. H.(2006).The acute effects of a caffeine-containing supplement on strength, muscular endurance, and anaerobic capabilities.Journal of Strength and Conditioning Research,20(3),506-510.
6. Bell, D. G.,Jacobs, I.,Zamecnik, J.(1998).Effects of caffeine, ephedrine and their combination on time to exhaustion during high-intensity exercise.European Journal of Applied Physiology,77,427-433.
7. Bell, D. G.,McClellan, T. M.(2002).Exercise endurance 1, 3, and 6 h after caffeine ingestion in caffeine users and nonusers.Journal of Applied Physiology,9,1227-1234.
8. Bell, D. G.,McClellan, T. M.(2003).Effect of repeated caffeine ingestion on repeated exhaustive exercise endurance.Medicine and Science in Sports and Exercise,35(8),1348-1354.
9. Bird, S. P.,Tarpenning, K. M.,Marino, F. E.(2006).Effects of liquid carbohydrate /essential amino acid ingestion on acute hormonal response during a single bout of resistance exercise in untrained men.Nutrition,22,367-375.
10. Blanchard, J.,Sawers, S. J.(1983).The absolute bioavailability of caffeine in man.European Journal of Clinical Pharmacology,24,93-98.
11. Bloomer, R. J.,Sforzo, G. A.,Keller, B. A.(2000).Effects of meal form and composition on plasma testosterone, cortisol, and insulin following resistance exercise.International journal of Sport Nutrition, Exercise and Metabolism,10,415-424.
12. Bonati, M.,Latini, R.,Galletti, F.,Young, J. F.,Tognoni, G.,Garattini, S.(1982).Caffeine disposition after oral doses.Clinical Pharmacology and Therapeutics,32,98-106.
13. Bush, J. A.,Kimball, S. R.,O’Connor, P. M,Suryawan, A.,Orellana, R. A.,Nguyen, H. V.,Davis, T. A.(2003).Translational control of protein synthesis in muscle and liver of growth hormone-treated pigs.Endocrinology,144,1273-1283.
14. Chandler, R. M.,Byrne, H. K.,Paterson, J. G.,Ivy, J. L.(1994).Dietary supplements affect the anabolic hormones after weight training exercise.Journal of Applied Physiology,76,839-845.
15. Denadai, B. S.,Denadai, M. L. D. R.(1998).Effects of caffeine on time to exhaustion in exercise performed below and above the anaerobic threshold.Brazilian Journal of Medical and Biological Research,31(4),581-585.
16. Dill, D. B.,Costill, D. L.(1974).Calculation of percentage changes in volumes of blood, plasma, and red cells in dehydration.Journal of Applied Physiology,37,247-248.
17. Duncan, M. J.,Oxford, S. W.(2011).The effect of caffeine ingestion on mood state and bench press performance to failure.Journal of Strength and Conditioning Research,25(1),178-185.
18. Fredholm, B. B.,Battig, K.,Holmen, J.,Nehlig, A.,Zvartau, E. E.(1999).Actions of caffeine in the brain with special reference to factors that contribute to its widespread use.Pharmacological Reviews,51,83-133.
19. Gavrieli, A.,Yannakoulia, M.,Fragopoulou, E.,Margaritopoulos, D.,Chamberland, J. P.,Kaisari, P.,Mantzoros, C. S.(2011).Caffeinated coffee does not acutely affect energy intake, appetite, or inflammation but prevents serum cortisol concentrations from falling in healthy men.Journal of Nutrition,141(4),703-707.
20. Goldstein, E.,Patrick, L. J,Michael, W.,Tina, P.,Antonio, J.(2010).Caffeine enhances upper body strength in resistance-trained women.Journal of the International Society of Sports Nutrition,7,18.
21. Graham, T. E.(2001).Caffeine and exercise: Metabolism, endurance and performance.Sports Medicine,31(11),785-807.
22. Graham, T. E.,Helge, J. W.,MacLean, D. A.,Kiens, B.,Richter, E. A.(2000).Caffeine ingestion does not alter carbohydrate or fat metabolism in human skeletal muscle during exercise.Journal of Applied Physiology,529,837-847.
23. Graham, T. E.,Spriet, L. L.(1995).Metabolic, catecholamine, and exercise performance responses to various doses of caffeine.Journal of Applied Physiology,78,867-874.
24. Green, J. M.,Wickwire, P. J.,McLester, J. R.,Gendle, S.,Hudson, G.,Pritchett, R. C.,Laurent, C. M.(2007).Effects of caffeine in repetitions to failure and ratings of perceived exertion during resistance training.International Journal of Sports Physiology and Performance,2,250-259.
25. Hansen, S.,Kvorning, T.,Kjaer, M.,Sjogaard, G.(2001).The effect of short term strength training on human skeletal muscle: The importance of physiologically elevated hormone levels.Scandinavian Journal of Medicine and Science in Sports,11,347-354.
26. Hulmi, J. J.,Volek, J. S.,Selanne, H.(2005).Protein ingestion prior to strength exercise affects blood hormones and metabolism.Medicine and Science in Sports and Exercise,37,1990-1997.
27. Kamimori, G. H.,Karyekar, C. S.,Otterstetter, R.(2002).The rate of absorption and relative bioavailability of caffeine administered in chewing gum versus capsules to normal healthy volunteers.International Journal of Pharmacology,234,159-167.
28. Kamimori, G. H.,Lugo, S. I.,Penetar, D. M.,Chamberlain, A. C.,Brunhart, G. E.,Brunhart, A. E.,Eddington, N. D.(1995).Dose-dependent caffeine pharmacokinetics during severe sleep deprivation in humans.International Journal of Clinical Pharmacology and Therapeutics,33,182-186.
29. Kamimori, G. H.,Penetar, D. M.,Headley, D. B.,Thorne, D. R.,Otterstetter, R.,Belenky, G.(2000).Effect of three caffeine doses on plasma catecholamines and alertness during prolonged wakefulness.European Journal of Clinical Pharmacology,56,537-544.
30. Kraemer, W. J.,Gordon, S. E.,Fleck, S. J.,Marchitelli, L. J.,Mello, R.,Dziados, J. E.,Fry, A. C.(1991).Endogenous anabolic hormonal and growth factor responses to heavy resistance exercise in males and females.International Journal of Sports Medicine,12,228-235.
31. Kraemer, W. J.,Hatfield, D. L.,Spiering, B. A.,Vingren, J. L.,Fragala, M. S.,Ho, J. Y.,Maresh, C. M.(2007).Effects of a multi-nutrient supplement on exercise performance and hormonal responses to resistance exercise.European Journal of Applied Physiology,101(5),637-646.
32. Kraemer, W. J.,Ratamess, N. A.(2005).Hormonal responses and adaptations to resistance exercise and training.Sports Medicine,35(4),339-361.
33. Lanzi, R.,Losa, M.,Mignogna, G.,Caumo, A.,Pontrioli, A. E.(1999).The control on growth hormone release by free fatty acids is maintained in acromegaly.Journal of clinical endocrinology and metabolism,84,1234-1238.
34. Leonard, T. K.,Watson, R. R.,Mohs, M. E.(1987).The effects of caffeine on various body systems: A review.Journal of the American Dietetic Association,87,1048-1053.
35. Lieberman, H. R.(2003).Nutrition, brain function and cognitive performance.Appetite,40,245-254.
36. Lovallo, W. R.,Farag, N. H.,Vincent, A. S.,Thomas, T. L.,Wilson, M. F.(2006).Cortisol responses to mental stress, exercise, and meals following caffeine intake in men and women.Pharmacology Biochemistry and Behavior,83(3),441-447.
37. McLean, C.,Graham, T. E.(2002).Effects of exercise and thermal stress on caffeine pharmacokinetics in men and eumenorrheic women.Journal of Applied Physiology,93,1471-1478.
38. Quabbe, H. J.,Luyckx, A. S.,Lage, M.,Schwarz, C.(1983).Growth hormone, cortisol, and glucagon concentrations during plasma free fatty acid depression: Different effects of nicotinic acid and an adenosine derivative.Journal of clinical endocrinology and metabolism,57,410-414.
39. Quinlan, P.,Lane, J.,Aspinall, L.(1997).Effects of hot tea, coffee, and water ingestion on physiological responses and mood: the role of caffeine, water and beverage type.Psychopharmacology,134,164-173.
40. Ratamess, N. A.,Hoffman, J. R.,Ross, R.,Shanklin, M.,Faigenbaum, A. D.,Kang, J.(2007).Effects of an amino acid/creatine energy supplement on the acute hormonal response to resistance exercise.International Journal of Sport Nutrition and Exercise Metabolism,17,608-623.
41. Ryan, E. J.,Chul-Ho, K.,Fickes, E. J.,Williamson, M.,Muller, M. D.,Barkley, J. E.,Glickman, E. L.(2013).Caffeine gum and cycling performance: A timing study.Journal of Strength and Conditioning Research,27(1),259-264.
42. Sawynok, J.(1998).Adenosine receptor activation and nociception.European Journal of Pharmacology,347,1-11.
43. Selvage, D. J.,Lee, S. Y.,Parsons, L. H.,Seo, D. O.,Rivier, C. L.(2004).hypothalamic-testicular neural pathway is influenced by brain catecholamines, but not testicular blood flow.Endocrinology,145(4),1750-1759.
44. Skinner, T. L.,Kenkins, D. G.,Taaffe, D. R.,Leveritt, M. D.,Coombes, J. S.(2013).Coinciding exercise with peak serum caffeine does not improve cycling performance.Journal of Science and Medicine in Sport,16,54-59.
45. Sokmen, B.,Armstrong, L. E.,Kraemer, W. J.,Casa, D. J.,Dias, J. C.,Judelson, D. A.,Maresh, C. M.(2008).Caffeine use in sports: Considerations for the athlete.Journal of Strength and Conditioning Research,22,978-986.
46. Tarpenning, K. M.,Wiswell, R. A.,Hawkins, S. A.(2003).CHO-induced blunting of cortisol response to weightlifting exercise in resistance-trained older men.European Journal of Sport Science,3,1-10.
47. Vaugeois, J. M.(2002).Signal transduction: Positive feedback from coffee.Nature,418,734-736.
48. Warren, G. L.,Park., N. D.,Maresca., R. D.,Mckibans., K. I.,Millard-Stafford, M. L.(2010).Effect of caffeine ingestion on muscular strength and endurance: A meta-analysis.Medicine and Science in Sports and Exercise,42(7),1375-1387.
49. Watt, M. J.,Holmes, A. G.,Steinberg, G. R.,Mesa, J. L.,Kemp, B. E.,Febbraio, M. A.(2004).Reduced plasma FFA availability increases net triacylglycerol degradation, but not GPAT or HSL activity, in human skeletal muscle.American Journal of Physiology Endocrinology and Metabolism,287,E120-E127.
50. Williams, A. G.,Ismail, A. N.,Sharma, A.,Jones, D. A.(2002).Effects of resistance exercise volume and nutritional supplementation on anabolic and catabolic hormones.European Journal of Applied Physiology,86,315-321.
51. Woolf, K.,Bidwell, W. K.,Carlson, A. G.(2008).The effect of caffeine as an ergogenic aid in anaerobic exercise.International Journal of Sport Nutrition and Exercise Metabolism,18,412-429.
52. Wu, B. H.(2015).Dose effects of caffeine ingestion on acute hormonal responses to resistance exercise.The Journal of Sports Medicine and Physical Fitness,55,1242-1251.
53. Wu, B. H.,Lin, J. C.(2010).Caffeine attenuates acute growth hormone response to a single bout of resistance exercise.Journal of Sports Science and Medicine,9(2),262-269.
54. Zaki, A.,Barrett-Jolley, R.(2002).Rapid neuromodulation by cortisol in the rat paraventricular nucleus: An in vitro study.British Journal of Pharmacology,137(1),87-97.