淺談RT-PCR技術及凋亡調控蛋白Bal-2，Bax在運動中的表現

A Brief Review of the RT-PCR Techniques and the Expressions of Apoptosis Regulation Proteins Bcl-2 and Bax in Exercise

10.6223/qcpe.2702.201306.0906

林作慶(Tso-Ching Lin)；賴永成(Young-Cheng Lai)；傅正思(Cheng-Sze Fu)

細胞凋亡 ； 分子生物 ； apoptosis ； molecular biology ； mRNA

中華體育季刊

27卷2期（2013 / 06 / 01）

145 - 151

繁體中文

細胞淋巴瘤／白血病2（Bcl-2）家族為細胞凋亡路徑中重要的調控蛋白，其中主要透過家族成員B細胞淋巴瘤／白血病2蛋白（Bcl-2）和Bcl-2-性關聯蛋白（Bax）互相之拮抗來控制粒線體通透性轉換孔的開放，進而促進或阻止粒線體釋放細胞素c進行一連串的凋亡調控反應。本文簡單介紹RT-PCR技術並整理相關研究文獻，藉由反轉錄聚合酶鍊反應（RT-PCR）技術所偵測Bcl-2和Bax之傳訊RNA表現量，以分子生物的層面觀察運動對凋亡調控蛋白Bcl-2和Bax的影響；並探討Bcl-2和Bax在細胞保護作用中所扮演的角色，以及不同型式的運動中Bcl-2和Bax表現的差異，藉以對運動所誘發的細胞凋亡現象及影響機制有更深入的認識。

Bel-2 family is considered as the most crucial regulation protein in the process of apoptosis. It attempts to regulate the mitochondrial permeability transition pore by applying the contradicting movements between members (Bcl-2 and Bax) of the family. Furthermore, it will enable or hinder the cytochrome C to process a series of apoptosis regulation. The discussion briefly introduced the RT-PCR techniques and reviewed the related literatures. It detected the expression level of mRNA in Bcl-2 and Bax by applying the RT-PCR techniques, which enabled the researcher to monitor how exercises affected the apoptosis regulation proteins Bcl-2 and Bax from a monocular science point of view. It also elaborated what roles Bcl-2 and Bax played in cell protection mechanism and the expression discrepancy between Bcl-2 and Bax in different types of exercise models. This paper is hoped to eventually seek in depth acknowledgements of apoptosis phenomenon mechanism induced by exercises.

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