運動誘發心肌熱休克蛋白70表現之性別差異

Exercise-induced expression of cardiac heat shock protein 70 in sex difference

10.3966/102473002016123004007

李淑玲(Shu-Lin Lee)；傅正思(Cheng-Sze Fu)；何應志(Ying-Chih Ho)

分子伴護子 ； 雌激素 ； 心血管保護作用 ； 運動訓練 ； molecular chaperon ； estrogen ； cardioprotection ； exetcise ； training

中華體育季刊

30卷4期（2016 / 12 / 01）

295 - 301

繁體中文

當細胞面臨壓力損傷時熱休克蛋白將可協助細胞修復，以維持細胞生理功能等機制，許多研究了解熱休克蛋白(特別是熱休克蛋白70家族)對心臟細胞保護作用之影響，亦有研究結果肯定運動誘發熱休克蛋白70表現量與心肌功能的保護作用與效益；而在運動後心肌細胞熱休克蛋白70反應卻有性別上的差異性，在急性運動刺激後，男性心肌熱休克蛋白70表現量明顯增加，在女性體內表現量卻無明顯改變，此差異主要與女性體內的雌激素對細胞結構的穩定作用、提升抗氧化能力與血管擴張情形有關；在長期規律運動訓練時，心肌熱休克蛋白70表現量在男性與女性體內皆明顯增加，因此，以熱休克蛋白70表現角度更是肯定規律運動對心血管健康的保護效益。

Heat shock proteins (HSPs) functions were to maintain cellular homeostasis, facilitate repair of injured cells and provide protection against future insults. Recent studies supported that heat shock protein (asHSP70) could be a cardioprotective agent, and the protein expression may increase with exercise. However, there has sex difference in the cardiac shock protein 70 express aftERacute exercise. While males show a robust increase in cardiac shock protein 70 following a single bout of exercise, females may not. The sex difference seems mediated by estrogen and testosterone. Estrogen limits the exercise-related disruptions to cell homeostasis because of its fast-acting roles in the cardiovascular, such as membrane stabilizing, antioxidant, or vasodilatory properties. Although sex differences in the HSP70 expression, exercise training, as a nonpharmacological intervention, that associated up-regulation of HSP70 may prove effective in providing cardioprotection.

1. Benjamin, I. J.,Christians, E.(2002).Exercise, estrogen, and ischemic cardioprotection by heat shock protein 70.Circulation Research,90(8),833-835.
2. Benjamin, I. J.,McMillan, D. R.(1998).Stress (heat shock) proteins: Molecular chaperones in cardiovascular biology and disease.Circulation Research,83(2),117-132.
3. Chen, H. B.,Zhang, X. C.,Cheng, Y. F.,Abdelnasir, A.,Tang, S.,Kemper, N.,Bao, E. D.(2015).Association of heat shock protein 70expression with rat myocardial cell damage during heat stress in vitro and in vivo.Genetics and Molecular Research,14(1),1994-2005.
4. Gunaldi, M.,Kocoglu, H.,Okuturlar, Y.,Gedikbasi, A.,Karabulut, M.,Alis, H.,Hursitoglu, M.(2015).Heat shock protein 70is a useful markERfor predicting colorectal cancer.Journal of Buon,20(6),1464-1470.
5. Leung, F. P.,Yung, L. M.,Laher, I.,Yao, X.,Chen, Z. Y.,Huang, Y.(2008).Exercise, vascular wall and cardiovascular diseases: An update (Part 1).Sports Medicine,38(12),1009-1024.
6. Liu, X.,Zhang, C.,Li, J.,Guo, W.,Yan, D.,Yang, C.,Shi, J.(2016).Heat shock protein 70inhibits cardiomyocyte necroptosis through repressing autophagy in myocardial ischemia/reperfusion injury.In Vitro Cellular & Developmental Biology - Animal,52(6),690-698.
7. Locke, M.,Noble, E. G.,Tanguay, R. M.,Feild, M. R.,Ianuzzo, S. E.,Ianuzzo, C. D.(1995).Activation of heat-shock transcription factor in rat heart aftERheat shock and exercise.American Journal of Physiology,268(6),C1387-1394.
8. Lollo, P. C.,Moura, C. S.,Morato, P. N.,Amaya-Farfan, J.(2013).Differential response of heat shock proteins to uphill and downhill exercise in heart, skeletal muscle, lung and kidney tissues.Journal of Sports Science & Medicine,12(3),461-466.
9. Melling, C. W.,Thorp, D. B.,Milne, K. J.,Krause, M. P.,Noble, E. G.(2007).Exercise-mediated regulation of HSP70 expression following aerobic exercise training.American Journal of Physiology - Heart and Circulatory Physiology,293(6),H3692-3698.
10. Melling, C. W.,Thorp, D. B.,Noble, E. G.(2004).Regulation of myocardial heat shock protein 70gene expression following exercise.Journal of Molecular and Cellular Cardiology,37(4),847-855.
11. Milne, K. J.,Noble, E. G.(2008).Response of the myocardium to exercise: Sex-specific regulation ofHSP70.Medicine and Science in Sports and Exercise,40(4),655-663.
12. Milne, K. J.,Thorp, D. B.,Krause, M.,Noble, E. G.(2011).Core temperature is a greatERinfluence than endogenous 17 beta-estradiol on the exercise-induced accumulation of myocardial heat shock protein mRNA.Canadian Journal of Physiology and Pharmacology,89(11),855-860.
13. Milne, K. J.,Wolff, S.,Noble, E. G.(2012).Myocardial accumulation and localization of the inducible 70-kDa heat shock protein, HSP70, following exercise.Journal of Applied Physiology,113(6),853-860.
14. Paroo, Z.,Haist, J. V.,Karmazyn, M.,Noble, E. G.(2002).Exercise improves postischemic Cardiac function in males but not females: Consequences of a novel sex-specific heat shock protein 70 response.Circulation Research,90(8),911-917.
15. Peng, W.,Zhang, Y.,Zheng, M.,Cheng, H.,Zhu, W.,Cao, C. M.,Xiao, R. P.(2010).Cardioprotection by CaMKII-deltaB is mediated by phosphorylation of heat shock factor 1 and subsequent expression of inducible heat shock protein 70 response.Circulation Research,106(1),102-110.
16. Powers, S. K.,Smuder, A. J.,Kavazis, A. N.,Quindry, J. C.(2014).Mechanisms of exercise-induced cardioprotection.Physiology,29(1),27-38.
17. Rani, N.,Bharti, S.,Manchanda, M.,Nag, T. C.,Ray, R.,Chauhan, S. S.,Arya, D. S.(2013).Regulation of heat shock proteins 27 and 70, p-Akt/p-eNOS and MAPKs by Naringin Dampens myocardial injury and dysfunction in vivo aftERischemia/reperfusion.PLoS One,8(12),e82577.
18. Ritossa, F.(1996).Discovery of the heat shock response.Cell Stress Chaperones,1(2),97-98.
19. Staib, J. L.,Quindry, J. C.,French, J. P.,Criswell, D. S.,Powers, S. K.(2007).Increased temperature, not cardiac load, activates heat shock transcription factor 1 and heat shock protein 72 expression in the heart.American Journal of Physiology - Regulatory, Integrative and Comparative Physiology,292(1),R432-439.
20. Staib, J. L.,Tumer, N.,Powers, S. K.(2009).Increased temperature and protein oxidation lead to HSP72 mRNA and protein accumulation in the in vivo exercised rat heart.Experimental Physiology,94(1),71-80.
21. Starnes, J. W.,Choilawala, A. M.,Taylor, R. P.,Nelson, M. J.,Delp, M. D.(2005).Myocardial heat shock protein 70 expression in young and old rats after identical exercise programs.Journals of Gerontology, Series A: Biological Sciences,60(8),963-969.
22. Su, X.,Sykes, J. B.,Ao, L.,Raeburn, C. D.,Fullerton, D. A.,Meng, X.(2010).Extracellular heat shock cognate protein 70induces cardiac functional tolerance to endotoxin: Differential effect on TNF-alpha and ICAM-1 levels in heart tissue.Cytokine,51(1),60-66.