異氰酸作業過敏勞工白血球DQ1基因類型

DQ1 Gene Types among Sensitized Workers Exposed to Isocyanates

10.7005/JOSH.200912.0424

熊映美(Yin-Mei Chiung)；司徒惠康(Hui-Kang Sytwu)；石東生(Tung-Sheng Shih)；蕭孟芳(Men-Faung Shaio)；郭育良(Yu-Lian Kuo)；姚振文(Chen-Wen Yao)

異氰酸 ； 過敏 ； 勞工 ； 白血球基因 ； Isocyanates ； Allergy ； Worker ； HLA gene

勞工安全衛生研究季刊

17卷4期（2009 / 12 / 01）

424 - 435

繁體中文

This study extracted nucleic acid from frozen blood samples collected from isocyanate salts operating workers who worked in the foam, synthetic leather and resins factories, analyzing complex antigens type II organization of human leukocyte antigen (Human Leukocyte Antigen, HLA) genotypes. Based on a questionnaire which inquired into the workers' consciously work-related allergy symptoms, we divided the respondents into deferent groups then counted various genes and the isocyanate allergy disease's relevance. Using 34 DQ1 high-resolution genetic primers for PCR reaction. We completed a total of 271 workers of the genotype analysis. Statistics of occurrence rate of the allele told that only 26 genotypes showed up. Among them, DQ1 0301 had the highest frequency of accounting (23.3%) and DQ1 0502 second (12.9%). The genes which were more associated with allergic syndromes, DQ1 0601 and 0503, had occurrence rates of approximately 5.0% and 3.3% (X^2, p＜0.05). The statistic results showed work-related allergic symptoms occurred in different parts of bodies, the relevant genotypes were also different. After adjusting for gender, age, exposure, years of service and smoking habit using logistic regression, DQ1 0503 and DQ1 0402 trigger respiratory allergies which caused by the working showed the highest odds ratio (OR) 5.79 and 4.66. DQ1 0601 with skin and eyes showed a significant correlation between the symptoms, but the OR after correction is less than 1. It shows that gene is not a key determinant in the above-mentioned cases of allergy of isocyanate sensitization. DQ1 0503 genotype and work-related wheezing symptoms were significantly associated uncorrected OR of 4.31 and the correction OR is 5.79, show the genetic impact on the respiratory allergies caused by isocyanates exposure. According to the above results, our study indicated that the DQ1 0503, 0402 and 0601 have important significance to isocyanate-induced allergic reactions.

This study extracted nucleic acid from frozen blood samples collected from isocyanate salts operating workers who worked in the foam, synthetic leather and resins factories, analyzing complex antigens type II organization of human leukocyte antigen (Human Leukocyte Antigen, HLA) genotypes. Based on a questionnaire which inquired into the workers' consciously work-related allergy symptoms, we divided the respondents into deferent groups then counted various genes and the isocyanate allergy disease's relevance. Using 34 DQ1 high-resolution genetic primers for PCR reaction. We completed a total of 271 workers of the genotype analysis. Statistics of occurrence rate of the allele told that only 26 genotypes showed up. Among them, DQ1 0301 had the highest frequency of accounting (23.3%) and DQ1 0502 second (12.9%). The genes which were more associated with allergic syndromes, DQ1 0601 and 0503, had occurrence rates of approximately 5.0% and 3.3% (X^2, p＜0.05). The statistic results showed work-related allergic symptoms occurred in different parts of bodies, the relevant genotypes were also different. After adjusting for gender, age, exposure, years of service and smoking habit using logistic regression, DQ1 0503 and DQ1 0402 trigger respiratory allergies which caused by the working showed the highest odds ratio (OR) 5.79 and 4.66. DQ1 0601 with skin and eyes showed a significant correlation between the symptoms, but the OR after correction is less than 1. It shows that gene is not a key determinant in the above-mentioned cases of allergy of isocyanate sensitization. DQ1 0503 genotype and work-related wheezing symptoms were significantly associated uncorrected OR of 4.31 and the correction OR is 5.79, show the genetic impact on the respiratory allergies caused by isocyanates exposure. According to the above results, our study indicated that the DQ1 0503, 0402 and 0601 have important significance to isocyanate-induced allergic reactions.

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