Celecoxib Did Not Impair Proliferation, Migration and F-actin Formation of Skeletal Muscle Cells

希樂葆不影響骨骼肌細胞增生、移行及F-actin組成

10.6315/TJPMR.202112_49(2).0005

陳航民(Hang-Ming Chen)；陳志嘉(Zhi-Jia Chen)；林莉萍(Li-Ping Lin)；蔡文鐘(Wen-Chung Tsai)；蘇中慧(Jong-Hwei S. Pang)

Celecoxib ； skeletal muscle cells ； 希樂葆（Celecoxib） ； 骨骼肌細胞（skeletal muscle cells）

台灣復健醫學雜誌

49卷2期（2021 / 12 / 01）

183 - 192

英文

Muscle injury is the most common sport related soft-tissue injury. Nonsteroidal anti-inflammatory drugs (NSAIDs) are commonly used for muscle injury treatment. The newest guideline (PEACE and LOVE) from British Journal of Sports Medicine in 2019 emphasized the avoidance of anti-inflammatory modalities in acute phase for the concerning about the negative effect on long-term tissue healing. It has been investigated that NSAIDs impeded cell migration which is crucial role in muscle healing. This study was designed to determine the effects of celecoxib, one of the cyclooxygenase-2 (COX-2) inhibitor on cell proliferation and migration in skeletal muscle cells. MTT assay (3-[4,5-dimethylthiazol- 2-yl]-2,5-diphenyltetrazolium bromide) was used to examine cell viability. In vitro wounding healing test, transwell filter migration assay and F-actin staining were adopted to evaluate cell migration and spreading. The results showed that celecoxib had no negative effect on in vitro wounding healing test and cell viability. Besides, celecoxib also did not impact cell migration and cell spreading. Despite the ＂PEACE and LOVE＂ guideline, celecoxib might be the better choice compared to the traditional NSAIDs for some inevitable situation due to their possible less influence on the muscle recovery process.

肌肉損傷為最常見的運動傷害之一，非固醇類抗發炎藥常用於肌肉損傷之治療，但2019年英國運動醫學期刊最新運動傷害處理原則（PEACE & LOVE）指出急性傷害期避免過度使用抗發炎藥，以免影響組織癒合。過去文獻指出非固醇類抗發炎藥會抑制肌肉細胞移行能力，此機制為肌肉癒合之關鍵。本研究目的為測試COX-2抑制劑希樂葆是否會影響肌肉細胞增生及移行能力。以MTT試驗檢測細胞增生能力，以體外傷口癒合試驗及細胞遷移實驗驗證細胞移行能力，以F-actin染色觀察細胞擴展能力。研究結果顯示希樂葆不會影響細胞增生及體外傷口癒合能力，此外希樂葆也不會影響細胞移行及擴展能力。儘管最新運動傷害處理原則（PEACE & LOVE）不建議使用抗發炎藥，但在不可避免的情況下，COX-2抑制劑希樂葆與其他抗發炎藥相比可能是更好的選擇。

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