Shikonin抑制人類黑色素細胞癌細胞株(A375.S2)之N基乙醯化2-AF

The Inhibitory Function of Shikonin on N-acetylation of 2-aminofluorene in Human Malignant Melanoma Cells (A375.S2)

10.29784/DS.200409.0001

袁上雯(Shang-Wen Yuan)；陳光偉(Guang-Wei Chen)；沈瑞隆(Jui-Lung Shen)；張惠玲(Huei-Lin Chang)；鍾景光(Jing-Gung Chung)

Shikonin ； Melanoma ； N-acetyltransferase NAT ； N-acetylation ； Arylamine 2-aminofluo-rene ； Lithospermum erythrorhizon

中華皮膚科醫學雜誌

22卷3期（2004 / 09 / 01）

201 - 207

繁體中文

Shikonin是紫草的主要成分之一，紫草在傳統中醫上常用於治療皮膚發炎及過敏。本研究的目的在探討shikonin如何影響人類黑色素細胞株(A375.S2)。利用高效液相層析儀(HPLC)分析人類黑色素細胞癌細胞株N基乙醯化2-aminofluorene的量，結果顯示shikonin可以減少N基乙醯的代謝產物(2-AAF)的產生，且此影響有計量依成性。並發現shikonin可抑制NATI基因(NAT mRNA)，為2-aminofluorene的Ｎ基乙醯能力降低的原因之一。本實驗為shikonin抑制人類黑色素細胞癌細胞株(A375.S2)的Ｎ基乙醯化2-aminofluorene之首例研究。

Shikonin is one of the components of Lithospermum erythrorhizon (Chinese herb medicine) used for skin infection and allergy for many generations in the Chinese population. In this study, shikonin was used to determine the inhibition of N-acetylation of 2-aminofluorene (2-AF) in human malignant melanoma cell line (A375.S2). The amounts of N-acetylation and non-N-acetylation of 2-AF were measured by high performance liquid chromatography (HPLC). The results demonstrated that N-acetylation of AF from examined systems were decreased by shikonin in a dose0dependent manner. We also found out that shikonin inhibited the expression of NAT1 gene. Apparently shikonin inhibited N-acetylation of 2-AF in human malignant melanoma cell line. These are the first findings to show shikonin affect N-acetylation of 2-aminofluorene of human malignant melanoma cell line (A375.S2).