義齒樹脂表面粗糙度對白色念珠菌黏附的影響

The Influence of Denture Surface Roughness on the Adhesion of Candida albicans

10.6320/FJM.2005.9(3).04

林佶弘(Chi-Hung Lin)；陳宜君(Yee-Chun Chen)；林立德(Li-Deh Lin)；王東美(Tong-Mei Wang)；王若松(Juo-Song Wang)

義齒表面粗糙度 ； 白色念珠菌黏附 ； denture surface roughness ； adhesion of Candida albicans

台灣醫學

9卷3期（2005 / 05 / 01）

332 - 338

繁體中文

The adhesion assays between Candida and denture acrylic in vitro ere commonly evaluated by microscopic examination, but the results could not be quantified easily. The purposes of our study were to evaluate the influences of surface roughness of denture acrylic on the adhesion of C.albicans by imprint culture technique. Forty pieces of heat-cured acrylic resin, 2x5x0.1 cm in size, were fabricated according to manufacturer's instruction. Ten pieces as a group, acrylic surfaces were polished by carbide, green stone, and big silicone burs respectively, and 10 pieces were done nothing as control. Before adhesion assay, surface roughness (Ra, Rz) on each resin plates were measured by Surftest 4. Then the resin plate vas immersed in a culture media which contain SC5314 (about 10^4 yeasts/ml). One hour later, the sample vas air dried and imprinted on Sabourand dextrose agar with constant loads, after 24 hours the forming colonies on the agar ere counted. The results showed that surface roughness was significantly different among groups (p＜0.00l). Although surface of control group vas not the roughest (Ra=1.06±0.22, Rz8.13±1.58), Candida adhesion (222±139 CFU/cm^2) vas significantly more than those of the experimental groups (p＜0.00l). In the experimental groups, green stone group showed the roughest surface (Ra=2.34±0.45. Rz=13.54±1.97), which also adhered significantly more Candida (76±43 CFU/cm^2) (p＜0.05). Although surface roughness beteen carbide (Ra=1.46±0.15, Rz=6.80±0.73) and big silicone groups (Ra=0.70±0.09, Rz=3.67±0.67) as significantly different (p＜0.001), there is no difference in Candida adhesion (carbite group=30±21 CFU/cm^2, big silicone group=32±I2CFU/cm^2)(p=0.99). Our results suggest that there may be factors other than surface roughness, which may also affect the adhesion of Candida to the denture surface.

The adhesion assays between Candida and denture acrylic in vitro ere commonly evaluated by microscopic examination, but the results could not be quantified easily. The purposes of our study were to evaluate the influences of surface roughness of denture acrylic on the adhesion of C.albicans by imprint culture technique. Forty pieces of heat-cured acrylic resin, 2x5x0.1 cm in size, were fabricated according to manufacturer's instruction. Ten pieces as a group, acrylic surfaces were polished by carbide, green stone, and big silicone burs respectively, and 10 pieces were done nothing as control. Before adhesion assay, surface roughness (Ra, Rz) on each resin plates were measured by Surftest 4. Then the resin plate vas immersed in a culture media which contain SC5314 (about 10^4 yeasts/ml). One hour later, the sample vas air dried and imprinted on Sabourand dextrose agar with constant loads, after 24 hours the forming colonies on the agar ere counted. The results showed that surface roughness was significantly different among groups (p＜0.00l). Although surface of control group vas not the roughest (Ra=1.06±0.22, Rz8.13±1.58), Candida adhesion (222±139 CFU/cm^2) vas significantly more than those of the experimental groups (p＜0.00l). In the experimental groups, green stone group showed the roughest surface (Ra=2.34±0.45. Rz=13.54±1.97), which also adhered significantly more Candida (76±43 CFU/cm^2) (p＜0.05). Although surface roughness beteen carbide (Ra=1.46±0.15, Rz=6.80±0.73) and big silicone groups (Ra=0.70±0.09, Rz=3.67±0.67) as significantly different (p＜0.001), there is no difference in Candida adhesion (carbite group=30±21 CFU/cm^2, big silicone group=32±I2CFU/cm^2)(p=0.99). Our results suggest that there may be factors other than surface roughness, which may also affect the adhesion of Candida to the denture surface.