散血草可恢復THP-1單核球細胞經微波輻射所抑制NFκB活化表現現象

Manybracteole Bugle Extract Shown to Rescue Protein Expression of Transcription Factor NFκB in THP-1 Monocytes That are Inhibited by Microwave Radiation

黃兆君(Chou-Chun Huang)；李晨宇(Chen-Yu Li)；蔡文翔(Wein-Shiang Tsai)；廖美華(May-Hua Liao)；唐存愷(Tswen-Kei Tang)

人類單核球細胞株（THP-1） ； 佛波醇12-十四酸酯13-乙酸酯（PMA） ； 脂多醣體（LPS） ； 細胞核轉錄因子（NF-B） ； 散血草 ； THP-1moncyte ； Phorbol 12-myristate 13-acetate (PMA) ； Lipopolysaccharides (LPS) ； Nuclear Factor - Kappa B (NF-B) ； Manybracteole Bugle extract

元培學報

20期（2013 / 12 / 01）

79 - 86

繁體中文

微波，是人們在日常生活中常會接觸到的，當世界衛生組織（World Health Organization-WHO）提出微波是一種微弱干擾人體生理功能的環境能量後，即引發人類開始思考微波是否會對人體生理功能造成傷害，單核球免疫力之表現與細胞中發炎因子NFκB的活性有密切相關。人類單核球細胞株THP-1細胞受到phorbol 12-myristate 13-acetate （PMA）作用促使 THP-1細胞分化成為巨噬細胞，接著再以 lipopolysaccharides （LPS）作用促進活化使THP-1細胞內NFκB蛋白質的量增加。在微波照射的頻率為2450 MHz 900W影響THP-1細胞NFκB蛋白質量降低。另外探討在微波照射後加入散血草對THP-1單核球細胞NFκB蛋白質表現。散血草具有強效調節活化免疫細胞，而中國草藥指出散血草具有抗發炎的效果。我們用西方墨點分析NFκB蛋白質表現在THP-1單核細胞。在我們的實驗中，100 nM PMA作用24小時刺激THP-1單核細胞分化成巨噬細胞，在立即微波照射的頻率為2450 MHz 900W，隨後分別加入散血草萃取液15 μg、30 μg、150 μg、三種不同劑量培養3小時後，接著再以1μg/ml LPS作用2小時，結果發現加了散血草萃取液的作用下NFκB蛋白質都會上升，由其是在散血草萃取液15μg濃度時有顯著的上升在NFκB蛋白質的表現。

Microwave radiation can be encountered regularly in daily lives. When World Health Organization (WHO) announced that microwave radiation is a kind of environmental energy which interferes with the physiological functions of human body, great concerns have been raised over the damage frequency can do to human physiology. The immunological performance and the activeness of cellular inflammatory factor NFκB have been closely related in monocytes. Due to the effect of phorbol 12-myristate 13-acetate (PMA) to THP-1 monocytes, THP-1 monocytes can divide into macrophages and then react with lipopolysaccharides (LPS), causing an increase in the amount of NFκB protein in THP-1 monocytes. The protein expression of NFκB decreases when cells are exposed to frequency at 2450 MHz at 900W. After addition of Manybracteole Bugle extract to THP-1 monocytes, protein expression of NFκB has shown to be rescued. Manybracteole Bugle is a Taiwanese folk medicine, and possesses anti-inflammatory effects. Analysis of NFκB protein expression in THP-1 monocytes has been done with Western Blotting. It was observed that under stimulation with 100 nM PMA for 24h, THP-1 monocytes were able to differentiate into macrophages. Furthermore, in the immediate frequency of microwave irradiation 2450 MHz at 900W, Manybracteole Bugle extract was added at three kinds different doses including 15μg, 30 μg, 150 μg for 3 hours, followed by 2 hours to effect 1 μg/ml LPS. It was found under the effect of addition of 15μg, 30 μg, 150 μg, Manybracteole Bugle extract rescue protein level of NFκB.