篩選以大豆為發酵基質生產血管收縮素轉換酶抑制能力最佳之乳酸菌株

Screening and characterization of the optimal Lactobacillus strain in soybean fermentation for angiotensin I converting enzyme inhibition activity

李勝箖(Sheng-Lin Li)；陳怡文( I-Wen Chen)；吳峻瑋(Chun-Wei Wu)；謝鈞任(Chun-Jen Hsieh)；馬雅均(Ma-Ya Chun)；朱紀實(Chi-Shih Chu)；羅至佑(Chih-Yu Lo)；呂英震(Ying-Chen Lu)

乳酸菌 ； 大豆 ； 血管收縮素轉換酶 ； 抗氧化 ； Lactic acid bacterial ； soybean ； angiotensin I converting enzyme ； antioxidant activity

嘉大農林學報

13卷1期（2016 / 02 / 01）

95 - 104

繁體中文

以乳酸菌發酵生產功能性胜肽，不但可以攝取益生菌和各種營養素，更可以有促進健康的功效。本研究篩選發酵大豆後抑制血管收縮素轉換晦能力最佳的乳酸菌株，並分析以大豆為基質之生長、產酸、大豆蛋白分解、血管收縮素轉換酶抑制、與自由基清除能力。結果顯示在測試的71株菌中，以LYC424發酵大豆產生的血管收縮素轉換酶抑制能力最強。分析以LYC424發酵大豆72小時菌數為7.80 logCFU/ml '酸鹼值為pH4.69，滴定酸度為2.37。隨時間增加蛋白質水解率增加，而血管收縮素轉換酶抑制能力也增加。在發酵72小時後，水溶性蛋白水解率為98.64%血管收縮素轉換晦抑制能力為58.26% 。發酵的過程中，發酵液之抗氧化能力也持續增加。在發酵72小時後，發酵液之自由基清除能力提高30.38 %。本研究發現LYC424可以在大豆中生長，分解大豆蛋白產生血管收縮素轉換酶抑制活性，並提高自由基清除能力，值得進一步進行試驗並開發成保健食品。

Functional peptides through lactic acid fermentation are benefic to human, not probiotics and various nutrients but also promote health. In this study, we analyzed a total of 71 lactic acid bacteria from the laboratory culture collections. Lactic acid bacteria were selected for their ability to inhibit angiotensin I-converting enzyme activity during soybean fermentation. The strain LYC424 was selected for its good inhibition of angiotensin I-converting enzyme activity. The concentration of soluble proteins and peptides, free radical scavenging activity and general physical and chemical properties were further analyzed for the strain LYC424. After 72-hour fennentation, the bacterial number was 7.80 10gCFU/ml with pH 4.69 and titratable acidity was 2.37. The degree of protein hydrolysis and angiotensin I-converting enzyme inhibition activity were increased with fermentation time. After 72-hour fermentation, the degree of protein hydrolysis was 98.64% and the inhibitory activity on angiotensin I-converting enzyme was 62.84%. The free radical scavenging activity increased 30.38% after 72-hour fermentation. In conclusion, LYC424 has a strong protein hydrolysis activity, and can not only effectively release the angiotensin I-converting enzyme inhibitory peptides but also increase free radical scavenging activity.