The Relationship Between Early-onset Childhood Obesity and POMC and UCP3 Gene Variance

POMC及UCP3基因多形性與台灣學童肥胖相關性之研究

10.6558/MTJM.2001.6(4).4

陳志玫(Chih-Mei Chen)；劉秋松(Chiu-Shong Liu)；王妙媛(Nancy M. Wang)；林正介(Cheng-Chieh Lin)

肥胖 ； 基因多形性 ； POMC ； UCP3 ； obesity ； polymorphism ； POMC ； UCP3

Mid-Taiwan Journal of Medicine

6卷4期（2001 / 12 / 01）

216 - 222

英文

背景：為暸解台灣學童之肥胖情形與POMC及UCP3之相關性。 方法：本研究以台中市之國小學童為樣本，收集其血液樣本粹取DNA，以BMI指數為指標選取肥胖者100名及非肥胖者300名為研究對象，利用聚合酵素鏈鎖反應(PCR)及限制酵素片斷多形性(RFLP)等方法來判定基因之基因型。 結果：肥胖者在POMC及UCP3兩基因之基因多形性(polymorphism)的分佈上與非肥胖者並無差異。 結論：POMC及UCP3兩基因在本研究所分析之基因多形性與台灣學童的肥胖無顯著的相關性。

Background: Human Uncoupling Protein 3 (UCP3) is a new candidate gene for human obesity. UCPJ has been shown to be regulated by thyroid hormone, 3-adrenergic agonists, leptin, and fat feeding in rodents. Mutations in exon 3 (V1O2I) and exon 4 (R143X) were identified in obese and diabetic pro bands. Pro-opiomeleancortin (POMC), another obese gene, plays a central role in α-MSH regulation of food intake by activating melanocortin-4-receptors in the brain. The POMCgene codes for the prohormone pro-opiomelanocortin, the hormone suspected of playing a role in appetite and body weight regulation. Methods: We selected 100 obese and 300 non-obese children for analysis. Obesity was defined as a body mass index greater than or equal to the 95percentile. To examine whether mutations in exon 3 (V1O2I) and 4 (R143X) of the uncoupling protein gene might be factors for obesity in Taiwanese children, the target DNA fragments were amplified by polymerase chain reaction, and the genotypes were defined by restriction fragment length polymorphism. Results: No significant differences in the frequency of these mutations were found between obese and non-obese children. Conclusions: The results indicate that those mutations of the UCP3 and POMC genes do not playa role in the development of obesity in Taiwan's children.