Flaxseed ethanolic crude extract influences growth of stem cells from human exfoliated deciduous teeth (SHED)

Nur Sazwi Nordin；Khairani Idah Mokhtar；Muhammad Lokman Md Isa；Syed Zahir Idid；Widya Lestari；Basma Ezzat Mustafa；Solachuddin Jauhari Arief Ikhwan；Deny Susanti Darnis；Azlina Ahmad；Thirumulu Ponnuraj Kannan

Stem cells ； flaxseed crude extract ； cell viability ； proliferation activity

Journal of International Dental and Medical Research

11卷2期（2018 / 07 / 01）

643 - 649

英文

Previous study reported that plant natural product promotes stem cell growth. Flaxseed (Linum usitatissimum) contains numerous compounds recognized for its health benefits. This study aimed to investigate the biological effects of flaxseed crude extract on stem cells obtained from human exfoliated deciduous teeth (SHED). Whole flaxseeds were ground and extracted with absolute ethanol using soxhlet extractor. The effects of flaxseed crude extract on SHED were assessed at concentration 1, 2, 4, 8 and 16 mg/ml for cell viability using MTT assay, cell morphology using inverted microscope and proliferative activity describe as population doubling time (PDT) using alamarBlue assay. The fatty acid composition of flaxseed was analysed using gas chromatographymass spectrometry (GCMS) instrumental technique.Although insignificant, flaxseed at concentration up to 4 mg/ml slightly increased SHED proliferation activity while maintaining cell viability and morphology. However, 8 mg/ml of flaxseed inhibited cell viability and proliferation activity, and changed the cell morphology. GCMS analysis revealed the presence of linolenic acid, linoleic acid, palmitic acid and stearic acid. Overall, ethanolic crude extract of flaxseed at concentration up to 4 mg/ml slightly enhanced the growth and maintained the morphology of SHED.