Gingival Mesenchymal Stem Cells from Wistar Rat's Gingiva (Rattus Novergicus) - Isolation and Characterization (In Vitro Study)

Alexander Patera Nugraha；Ida Bagus Narmada；Diah Savitri Ernawati；Agung Dwi Wahyu Widodo；Pudji Lestari；Aristika Dinaryanti；Eryk Hendrianto；Igo Syaiful Ihsan；Helen Susilowati；Nora Ertanti；Deya Karsari；Fedik Abdul Rantam

Gingival Mesenchymal Stem Cells ； Immunophenotyping ； Isolation and Characterization ； Cluster of Differentiation ； Periodontal Tissue

Journal of International Dental and Medical Research

11卷2期（2018 / 07 / 01）

694 - 699

英文

Gingiva is emerging as a source of Mesenchymal Stem Cells. Gingival Mesenchymal Stem Cell has been isolated and characterized from the gingival connective tissue of wistar rat (Rattus Novergicus). Gingival Mesenchymal Stem Cell sources are rich, attainable and easy to isolate through minimal invasive procedure. Gingival Mesenchymal Stem Cells are ideal to accelerate bone regeneration. The aim of this study was to analyze Gingival Mesenchymal Stem Cells from Wistar Rats' gingiva (Rattus Novergicus) isolation and characterization by CD34, CD44, CD73, CD90, CD105 expression. This study was descriptive observational with simple random sampling method. Gingival Mesenchymal Stem Cells were isolated from healthy, 200 gram, 1 month year old, male rat's (Rattus Novergicus) lower gingival tissue through gingivectomy procedure (n=4). Gingiva were minced into small fragments then cultured in 2 weeks. The culture was passaged every 3-5 days after cultured and plated. The isolated Gingival Mesenchymal Stem Cells in passage 5 were characterized by CD34, CD44, CD73, CD90, CD105 using Immunocytochemistry and flowcytometry examination. Gingival Mesenchymal Stem Cells strongly expressed CD44+, CD73+, CD90+, CD105+ but did not express CD45- and CD34-. Gingival Mesenchymal Stem Cells' morphology was fibroblast-like, spindle-shaped, colony-forming abilities, and stick to the culture plate. Gingiva is potential Stem Cell source. Gingival Mesenchymal Stem Cells has multipotency ability with proliferation and mesenchymal stem cells characteristic advantageous for tissue engineering and regenerative therapy.