Potential Activity of Fraction of Piper Betle Linn Against Aggregatibacter Actinomycetemcomitans

Rusyanti Yanti；Rusminah N；Hendiani I；Komara I

Fraction ； Piper Betle Linn ； Aggregatibacter ； Actinomycetemcomitans

Journal of International Dental and Medical Research

11卷2期（2018 / 07 / 01）

700 - 706

英文

Aggregatibacter actinomycetemcomitans (Aa) is a potentially periodontal bacteria causing periodontal tissue destruction, which may not be cleaned at the time of scaling and root planing. Piper betle Linn which has long been used as a traditional medicine in Indonesia to cure various diseases in the mouth, potentially also as an adjunctive therapy in periodontitis. The objective of the research was to investigate the effect of betel leaf fraction on the growth of Aa bacteria. Materials and methods: Fresh betel leaf extraction with water, n-hexane, ethyl acetate and ethanol and 95% ethanol extract (EtOH) showed the highest inhibitory effect against Aa bacteria. 95% EtOH fractionated yielded 6 active fractions, then tested its antibacterial activity against Aa. Results: Fractionation of EtOH 95% yielded 6 active fractions comprising FrEtOH 30%, 50%, 100%, 10%, 20% and FW 100%. The test results of the 95% EtOH fraction showed 4 fractions that have inhibitory power against Aa bacteria. Conclusion: The 100% EtOH fraction shows the highest inhibitory effect against Aa bacteria.