運動調控基因表現：與健康相關性之研究

The Exercise Regulating Gene Expression: The Relationship with Health

10.5297/ser.200506_7(2).0023

李麗珍(Li-Chen Lee)；陳慕聰(Mu-Tsung Chen)；黃宏裕(Hung-Yu Huang)；陳宗與(Chung-Yu Chen)；蘇福新(Fu-Hsin Su)

運動 ； 基因表現 ； 健康 ； 基因晶片 ； exercise ； gene expression ； health ； microarray

大專體育學刊

7卷2期（2005 / 06 / 30）

287 - 299

繁體中文;英文

先前的流行病學研究顯示，運動與身體的健康狀態具有高度的關聯性，但其詳細的分子機轉仍尚未被瞭解。目前基因晶片技術已經逐漸成熟，然而是否能夠利用基因晶片作為有效的分析工具用於探討運動與基因表現間的關聯性，目前仍不清楚。50毫升的血液樣本抽取自規律運動習慣者與靜態生活型態者之靜脈血液，經純化RNA後，以含有人類8,000點基因之ABC Human Universo-Chip基因晶片進行cDNA microarray技術進行基因表現分析。結果發現，有規律運動習慣者在預防感冒、抑制癌症發生與血紅素合成相關基因表現顯著高於無規律運動習慣者；然而，規律運動與否與這些基因的關聯性仍須待後續系統性的研究進行聯結與證實。

Previous epidemiological studies have shown that exercise is closely associated with the whole body health condition, whereas the molecular-based evidence of this interaction still has not been adequately understood. Recently, the gene microarray technology has already matured for clinical practice; however, whether gene microarray could be a reliable analytical tools for discovering the relationship between exercise and health remains unclear. We obtained 15 mL venous whole blood from two different group of subjects with various physical activity levels (regular physical activity and sedentary). After we extracted the total RNA from the blood samples, we analyzed the disease-related gene expression by the microarray kit with 8,000 genes (ABC Human Universo-Chip). We found that the expression of those genes related to preventing influenza, inhibiting tumor growth, and hemoglobin synthesis are significantly higher in regular exercise groups than the sedentary ones. However, whether exercise really results in those benefits requires further systematic studies.

1. Boord, J. B.,Fazio, S.,Linton, M. F.(2002).Cytoplasmic fatty acid-binding proteins:Emerging roles in metabolism and atherosclerosis.Current Opinion in Lipidology,13(2),141-147.
2. Booth, F. W.,Chakravarthy, M. V.,Gordon, S. E.,Spangenburg, E. E.(2002).Waging war on physical inactivity: Using modem molecular ammunition against an ancient enemy.J. Appl. Physiol.,93(1),3-30.
3. Boule, N. G.,Haddad E.,Kenny, G. P.,Wells, G. A.,Sigal, R. J.(2001).Effects of exercise on glycemic control and body mass in type 2 diabetes mellitus: A meta-analysis of controlled clinical trials.JAMA,286(10),1218-1227.
4. Bronikowski, A. M.,Carter, P. A.,Morgan, T. J.,Garland, T.,Ung Jr, N.,Pugh, T. D.,Weindruch, R.,Prolla, T. A.(2003).Lifelong voluntary exercise in the mouse prevents age-related alterations in gene expression in the heart.Physiol. Genomics,12,129-138.
5. Calvert, P. M.,Frucht, H(2002).The genetics ofcolorectal cancer.Ann. Intern. Med.,137,603-612.
6. Castaneda, C.(2003).Diabetes control with physical activity and exercise.Nutrition in Clinical Care,6(2),89-96.
7. Difilippantonio, S.,Chen, Y.,Pietas, A.,Schluns, K.,Pacyna-Gengelbach, M.,Deutschmann, N.,Padilla-Nash, H. M.,Ried, T.,Petersen, I.(2003).Gene expression profiles in human non-small and small-cell lung cancers.Eur. J. Cancer,39(13),1936-1947.
8. Duggan, D. J.,Bittner, M.,Chen, Y.,Meltzer, P.,Trent, J. M.(1999).Expression profiling using cDNA microarrays.Nature Genetics,21,10-14.
9. Eisen, M.,Brown, P.(1999).DNA arrays for analysis of gene expression.Methods Enzymol,307,179-205.
10. Fehrenbach, E.,Zieker, D.,Niess, A. M.,Moeller, E.,Russwurm, S.,Northoff, H.(2003).Microarray technology-the future analyses tool in exercise physiology?.Exercise Immunology Review,9,58-69.
11. Folsom, R. W.,Littlefield-Chabaud, M. A.,French, D. D.,Pourciau, S. S.,Mistric, L.,Horohov, D. W.(2001).Exercise alters the immune response to equine influenza virus and increases susceptibility to infection.Equine Vet. J.,33(7),664-669.
12. Gallagher, P. G.(2003).Regulation of erythrocyte membrane protein gene expression.Curr. Opin. Hematol.,10(2),115-122.
13. Haller, 0.,Kochs, G.(2002).Interferon-induced mx proteins: Dynamin-like GTPases with antiviral activity.Traffic,3(10),710-717.
14. Ivy, J. L.,Zderic, T. W.,Fogt, D. L.(1999).Prevention and treatment of non-insulin-dependent diabetes mellitus.Exercise & Sport Sciences Reviews,27,1-35.
15. Lee, S. H.,Vidal, S. M.(2002).Functional diversity ofMx proteins: Variations on a theme of host resistance to infection.Genome Res,12(4),527-530.
16. Li, S.,Hursting, S. D.,Davis, B. J.,McLachlan, J. A.,Barrett, J. C.(2003).Environmental exposure, DNA methylation, and gene regulation: Lessons from diethylstilbesterol induced cancers.Annals of the New York Academy of Sciences,983,161-169.
17. Lopez-Borges, S.,Lazo, P. A.(2000).The human vaccinia-related kinase 1 (VRK1) phosphorylates threonine-18 within the mdm-2 binding site of the p53 tumour suppressor protein.Oncogene,19(32),3656-3664.
18. McElhaney, J. E.(2002).Influenza: a preventable lethal disease.J. Gerontol A. Biol. Set. Med. Sci.,57(10),M627-M628.
19. Nieman, D. C.(1997).Exercise immunology: Practical applications.International Journal of Sports Medicine,18(1),S91-S100.
20. Perusse, L.,Rankinen, T.,Rauramaa, R.,Rivera, M. A.,Wolfarth, B.,Bouchard, C.(2003).The human gene map for performance and health-related fitness phenotypes: The 2002 update.Med. Sci. Sports Exerc.,35(8),1248-1264.
21. Roth, S. M.,Ferrell, R. E.,Peters, D. G.,Metier, E. J.,Hurley, B. F.,Rogers, M. A.(2002).Influence of age, sex, and strength training on human muscle gene expression determined by microarray.Physiol. Genomics,10(3),181-190.
22. Segal, R. J.,Reid, R. D.,Coumeya, K. S.,Malone, S. C.,Parliament, M. B.,Scott, C. G.,Venner, P. M.,Quinney, H. A.,Jones, L. W.,D''Angelo, M. E.,Wells, G. A.(2003).Resistance exercise in men receiving androgen deprivation therapy for prostate cancer.J. Clin. Oncol,21(9),1653-1659.
23. Shih, C. H.,Chen, S. L.,Yen, C. C.,Huang, Y. H.,Chen, C.,Lee, Y. S.,Lin, K. H.(2004).Thyroid hormone receptor-dependent transcriptional regulation of fibrinogen and coagulation proteins.Endocrinology,145(6),2804-2814.
24. Singletary, S. E.(2003).Rating the risk factors for breast cancer.Annals of Surgery,237(4),474-482.
25. Vander, A.,Shareman, S.,Luciano, D.(2001).Human physiology: The mechanism of body function.New York:McGraw Hill.
26. Voogd, A. C.(2003).Exercise training improves cardiopulmonary function and quality of life in postmenopausal breast cancer survivors.Aust. J. Physiother,49(3),220.
27. Westerlind, K. C.(2003).Physical activity and cancer prevention-mechanisms..Med. Sci. Sports Exerc,35(11),1834-1840.
28. Westerlind, K. C.,McCarty, H. L.,Schultheiss, P. C.,Story, R.,Reed, A. H.,Baier, M. L.,Strange, R.(2003).Moderate exercise training slows mammary tumour growth in adolescent rats.Eur. J. Cancer Prev.,12(4),281-287.
29. Winett, R. A.,Carpinelli, R. N.(2001).Potential health-related benefits of resistance training.Prev. Med.,33(5),503-513.
30. Yoshioka, M.,Tanaka, H.,Naoko, S.,Snyder, E. E.,Shino, M.,St-Amand, J.(2003).Serial analysis of gene expression in the skeletal muscle of endurance athletes compared to sedentary men.FASEBJ,17(13),1812-1819.
31. Zakin, M. M.,Baron, B.,Guillou, F.(2002).Regulation of the tissue-specific expression of transfemn gene.Developmental Neuroscience,24(2-3),222-226.

1. 羅元鴻、金明央(2009)。基因功能表現差異對於個人飲食及運動介入的影響。長榮運動休閒學刊，3，34-42。
2. 孫佳婷(2021)。基因科技／基因禁藥研究：臺灣運動領域之認識架構。運動教練科學，61，41-51。
3. 魏麗敏、黃德祥、王宏益(2014)。國小教師運動參與、情緒智力與工作壓力關係之研究。運動休閒餐旅研究，9(4)，88-102。