低氧訓練對遺傳性肥胖動物血清瘦體素的影響

Effects of Hypoxia Training on Plasma Leptin in Genetically Obese Animals

10.5297/ser.200909_11(3).0010

侯建文(Chien-Wen Hou)；蔡櫻蘭(Ying-Lan Tsai)；吳茂盛(Mao-Sheng Wu)；葉益銘(Yi-Ming Yeh)；程一雄(I-Shiung Cheng)；陳瑞蓮(Jui-Lien Chen)；許東雄(Tung-Hsiung Hsu)

攝食量 ； 體重 ； 脂肪量 ； 口服葡萄糖耐受度測驗 ； food intake ； body weight ； fat mass ； OGTT

大專體育學刊

11卷3期（2009 / 09 / 30）

131 - 141

繁體中文;英文

本研究目的欲探討低氧加運動對肥胖基因鼠(obese Zucker rat)血清瘦體素、血糖吸收能力及肥胖程度的影響。胖、瘦各32隻體重約100克的Zucker雄鼠，分別給予兩組老鼠8週低氧、運動或低氧加運動的介入，測量老鼠攝食量、體重、脂肪量、血清瘦體素及血糖吸收能力。本研究結果顯示：低氧加運動能有效將Zucker胖鼠的攝食量降低至與Zucker瘦鼠相同，運動只有減少Zucker胖鼠的體重，但低氧加運動可以減少Zucker瘦、胖鼠的體重。運動減少胖和Zucker瘦鼠的脂肪量，但低氧加運動對脂肪量的減少沒有加成的效果。運動降低Zucker瘦、胖鼠血清瘦體素濃度，且運動加低氧可以有加成的效果。運動改善Zucker胖鼠血糖的控制能力，低氧加運動對Zucker瘦、胖鼠的血糖吸收能力皆有加成的效果。本研究結論顯示低氧加運動可以更有效的減肥和改善血糖吸收能力，未來可以使用低氧合併運動來改善第二型糖尿病病患的肥胖程度及血糖吸收能力。

Purpose: This study was aimed to examine the interactive effects of hypoxia plus exercise training on obese level, serum leptin and glucose uptake in obese and lean Zucker rats. Methods: Sixty-four Zucker rats both lean (n=32) and obese (n=32) were divided into 8 groups such as, lean control (LC, n=8), lean hypoxia (LH, n=8), lean exercise (LE, n=8), lean hypoxia + exercise (LHE, n=8), obese control (OC, n=8), obese hypoxia (OH, n=8), obese exercise (OE, n=8) and obese hypoxia + exercise (OHE, n=8). In all groups food intake, bodyweight, fat mass, serum leptin and glucose uptake were measured after the treatment. Results: The food intake was higher in obese control group and this was significantly decreased with hypoxia plus exercise treatment which is similar to the LHE group. We observed reduces bodyweight only in obese exercised group, however, hypoxia with exercise resulted in both lean and obese groups. Exercise also reduces the fat mass in both lean and obese groups whereas, no change with interaction of hypoxia plus exercise treatment. Serum leptin levels were significantly decreased with exercise and also with hypoxia plus exercise treatments. Glucose uptake was improved with hypoxia plus exercise treatment in both lean and obese groups. Conclusion: These results indicate that hypoxia with exercise training considered as more effective on reducing fat mass and improving glucose uptake. This data suggest that interactive treatment may be a suitable strategy to control the obese degree and blood glucose level in obese population.

1. Applegate, E. A.,Upton, D. E.,Stern, J. S.(1984).Exercise and detraining: Effect on food intake, adiposity and lipogenesis in Osborne-Mendel rats made obese by a high fat diet.Journal of Nutrition,114(2),447-459.
2. Barnes, B. R.,Ryder, J. W.,Steiler, T. L.,Fryer, L. G. D.,Carling, D.,Zierath, J. R.(2002).Isoform-specific regulation of 5' AMP-activated protein kinase in skeletal muscle from obese Zucker (fa/fa) rats in response to contraction.Diabetes,51(9),2703-2708.
3. Bigard, A. X.,Sanchez, H.,Birot, O.,Serrurier, B.(2000).Myosin heavy chain composition of skeletal muscles in young rats growing under hypobaric hypoxia conditions.Journal of Applied Physiology,88(2),479-486.
4. Christ, C. Y.,Hunt, D.,Hancock, J.,Garcia-Macedo, R.,Mandarino, L. J.,Ivy, J. L.(2002).Exercise training improves muscle insulin resistance but not insulin receptor signaling in obese Zucker rats.Journal of Applied Physiology,92(2),736-744.
5. Dolkas, C. B.,Rodnick, K. J.,Mondon, C. E.(1990).Effect of body weight gain on insulin sensitivity after retirement from exercise training.Journal of Applied Physiology,68(2),520-526.
6. Donnelly, J. E.,Hill, J. O.,Jacobsen, D. J.,Potteiger, J.,Sullivan, D. K.,Johnson, S. L.(2003).Effects of a 16-month randomized controlled exercise trial on body weight and composition in young, overweight men and women: The midwest exercise trial.Archives of International Medicine,163(11),1343-1350.
7. Flores, M. B. S.,Fernandes, M. F. A.,Ropelle, E. R.,Faria, M. C.,Ueno, M.,Velloso, L. A.(2006).Exercise improves insulin and leptin sensitivity in hypothalamus of Wistar rats.Diabetes,55(9),2554-2561.
8. Fushiki, T.,Kano, T.,Ito, K.,Hirofuji, C.,Inoue, K.,Moritani, T.(1992).Effects of chronic hypoxia on the whole-body insulin action in rats.Canadian Journal of Physiology and Pharmacology,70(11),1522-1524.
9. Ishii, T.,Yamakita, T.,Yamagami, K.,Yamamoto, T.,Miyamoto, M.,Kawasaki, K.(2001).Effect of exercise training on serum leptin levels in type 2 diabetic patients.Metabolism,50(10),1136-1140.
10. Kraemer, R. R.,Chu, H.,Castracane, V. D.(2002).Leptin and exercise.Experimental Biology and Medicine,227(9),701-708.
11. Landt, M.,Gingerich, R. L.,Havel, P. J.,Mueller, W. M.,Schoner, B.,Hale, J. E.(1998).Radioimmunoassay of rat leptin: Sexual dimorphism reversed from humans.Clinical Chemistry,44(3),565-570.
12. Levin, B. E.,Dunn-Meynell, A. A.(2004).Chronic exercise lowers the defended body weight gain and adiposity in diet-induced obese rats.American Journal of Physiology. Regulatory, Integrative and Comparative Physiology,286(4),771-778.
13. Meissner, U.,Hanisch, C.,Ostreicher, I.,Knerr, I.,Hofbauer, K.-H.,Blum, W. F.(2005).Differential regulation of leptin synthesis in rats during short-term hypoxia and short-term carbon monoxide inhalation.Endocrinology,146(1),215-220.
14. Miyasaka, K.,Ichikawa, M.,Kawanami, T.,Kanai, S.,Ohta, M.,Sato, N.(2003).Physical activity prevented age-related decline in energy metabolism in genetically obese and diabetic rats, but not in control rats.Mechanisms of Aging and Development,124(2),183-190.
15. Netzer, N. C.,Chytra, R.,Kupper, T.(2008).Low intense physical exercise in normobaric hypoxia leads to more weight loss in obese people than low intense physical exercise in normobaric sham hypoxia.Sleep Breath,12(2),129-134.
16. Nolte, L. A.,Hansen, P. A.,Chen, M. M.,Schluter, J. M.,Gulve, E. A.,Holloszy, J. O.(1998).Short-term exposure to tumor necrosis factor-alpha does not affect insulinstimulated glucose uptake in skeletal muscle.Diabetes,47(5),721-726.
17. Pagano, C.,Marzolo, M.,Granzotto, M.,Ricquier, D.,Federspil, G.,Vettor, R.(1999).Acute effects of exercise on circulating leptin in lean and genetically obese fa/fa rats.Biochemical and Biophysical Research Communications,255(3),698-702.
18. Park, J.-J.,Berggren, J. R.,Hulver, M. W.,Houmard, J. A.,Hoffman, E. P.(2006).GRB14, GPD1, and GDF8 as potential network collaborators in weight loss-induced improvements in insulin action in human skeletal muscle.Physiological Genomics,27(2),114-121.
19. Polotsky, V. Y.,Li, J.,Punjabi, N. M.,Rubin, A. E.,Smith, P. L.,Schwartz, A. R.(2003).Intermittent hypoxia increases insulin resistance in genetically obese mice.Journal of Physiology,552(1),253-264.
20. Rahmouni, K.,Morgan, D. A.,Morgan, G. M.,Mark, A. L.,Haynes, W. G.(2005).Role of selective leptin resistance in diet-induced obesity hypertension.Diabetes,54(7),2012-2018.
21. Roden, M.,Price, T. B.,Perseghin, G.,Petersen, K. F.,Rothman, D. L.,Cline, G. W.(1996).Mechanism of free fatty acid-induced insulin resistance in humans.Journal of Clinical Investigation,97(12),2859-2865.
22. Runyan, T. J.(1986).Adipocyte diameter distributions and body fat contents of sedentary and exercised female Zucker rats.Growth,50(1),95-101.
23. Scarpace, P. J.,Matheny, M.,Tumer, N.,Cheng, K. Y.,Zhang, Y.(2005).Leptin resistance exacerbates diet-induced obesity and is associated with diminished maximal leptin signalling capacity in rats.Diabetologia,48(6),1075-1083.
24. Seals, D. R.,Hagberg, J. M.,Allen, W. K.,Hurley, B. F.,Dalsky, G. P.,Ehsani, A. A.(1984).Glucose tolerance in young and older athletes and sedentary men.Journal of Applied Physiology,56(6),1521-1525.
25. Sharma, A.,Singh, S. B.,Panjwani, U.,Yadav, D. K.,Amitabh, K.,Singh, S.(2002).Effect of a carbohydrate supplement on feeding behaviour and exercise in rats exposed to hypobaric hypoxia.Appetite,39(2),127-135.
26. Simler, N.,Grosfeld, A.,Peinnequin, A.,Guerre-Millo, M.,Bigard, A.-X.(2006).Leptin receptor-deficient obese Zucker rats reduce their food intake in response to hypobaric hypoxia.American Journal of Physiology. Endocrinology and Metabolism,290(3),591-597.
27. Volpe, S. L.,Kobusingye, H.,Bailur, S.,Stanek, E.(2008).Effect of diet and exercise on body composition, energy intake and leptin levels in overweight women and men.Journal of the American College of Nutrition,27(2),195-208.
28. Wilcox, A. R.(1982).The effects of caffeine and exercise on body weight, fat-pad weight, and fat-cell size.Medicine and Science in Sports and Exercise,14(4),317-321.
29. You, T.,Murphy, K. M.,Lyles, M. F.,Demons, J. L.,Lenchik, L.,Nicklas, B. J.(2006).Addition of aerobic exercise to dietary weight loss preferentially reduces abdominal adipocyte size.International Journal of Obesity,30(8),1211-1216.

1. 謝錦城、廖威彰(2010)。運動介入對糖尿病前期狀態之改善。大專體育，111，97-103。