年齡對運動誘發固醇類荷爾蒙反應之影響：脫氫異雄固酮所扮演的角色

Effects of Age on Exercise Response in Steroid Hormones: Role of DHEA-S

10.5297/ser.1404.011

陳九州(Chiu-Chou Chen)；陳宗與(Chung-Yu Chen)；侯建文(Chien-Wen Hou)；王韻嘉(Yun-Chia Wang)；劉德智(Te-Chih Liu)

中年 ； 乳酸 ； 睪固酮 ； middle-aged ； lactate ； testosterone

大專體育學刊

14卷4期（2012 / 12 / 31）

500 - 511

繁體中文;英文

脫氫異雄固酮（dehydroepiandrosterone, DHEA）是許多固醇類荷爾蒙的共同前驅物，隨年齡增加而減少。本研究目的為探討老化對於120秒單次高強度腳踏車運動挑戰後固醇類荷爾蒙反應之影響，以及脫氫異雄固酮所扮演的角色。11名受試者包括年輕（n = 5，21.0 ± 0.4 歲）與中年男性（n = 6，48.3 ± 2.5 歲）；在補充脫氫異雄固酮（50 mg）後12小時接受120秒，強度為100%攝氧量峰值（V O2peak）之單次腳踏車運動挑戰。血中脫氫異雄固酮─硫酸鹽、乳酸、總睪固酮、游離睪固酮、促黃體生成激素（luteinizing hormone, LH）及可體松（cortisol） 濃度分別於運動挑戰前與第120秒後收集血液樣本並分析；結果顯示：本研究之120秒運動挑戰後使受試者之血乳酸濃由0.65 mM上升達到12 mM，但總睪固酮與游離睪固酮濃度在運動後均未顯著提高；單次補充50 mg之脫氫異雄固酮雖對運動過程第120秒總睪固酮濃度無明顯提升效果，但能提高游離睪固酮濃度。單次補充脫氫異雄固酮後12小時即可使兩年齡組血中脫氫異雄固酮─硫酸鹽與游離睪固酮濃度顯著升高，並且使中年男性血中游離睪固酮濃度達到年輕組之水準，而脫氫異雄固酮─硫酸鹽濃度更高於年輕男性3倍。中年組之促黃體生成激素明顯較年輕組高。中年補充脫氫異雄固酮組雖明顯提高了游離睪固酮濃度，但對於促黃體生成激素的濃度無明顯影響，且在運動挑戰後亦無法明顯提高促黃體生成激素濃度。本研究結論：在腳踏車測功儀進行高強度間歇單次運動過程後，雖增加血乳酸達18倍，但無法增加血睪固酮濃度；本研究發現年齡較高者男性促黃體生成激素濃度相對較高可能與游離睪固酮有關。此外，提高脫氫異雄固酮─硫酸鹽濃度並無法改變運動後所有固醇性荷爾蒙的反應。

DHEA, the common precursor of many steroid hormones, declines with advancing age. This study examined the effects of age on exercise response in steroid hormones. Eleven volunteers, including 5 young (21.0 ± 0.4 years) men and 6 middle-aged (48.3 ± 2.5 years) men, were supplemented with a single dose DHEA (50 mg) or placebo, 12 h before an 120-s of exercise challenge on a cycloergometer at 100% V4 O2max. Blood DHEA-S, lactate, testosterone, free testosterone and cortisol levels were measured before and after exercise challenge. Exercise significantly elevated the lactate levels from 0.65 to 12 mM immediately after cycling for both young and middle-aged subjects. However, total and free testosterone levels were not significantly changed after the 120-s high intensity cycling exercise. A single dose DHEA supplementation significantly affected free testosterone in parallel with a 3-fold elevation in DHEA-S concentration for the middle-aged subjects to that of the youth level. Luteinizing hormone (LH) levels in middle-aged subjects were significantly greater than that in the young subjects. Despite DHEA supplementation elevated free testosterone levels, LH levels remained unaffected. Furthermore, exercise has no significant effect on LH levels. Conclusion: Despite lactate levels increased by 18-fold, exercise was unable to increase testosterone levels. Additionally, we found elevated LH levels in middleaged subjects may be associated with reduced free testosterone. Furthermore, increasing DHEA-S could not affect exercise response to steroid hormone.

1. Arlt, W.,Haas, J.,Callies, F.,Reincke, M.,Hubler, D.,Oettel, M.(1999).Biotransformation of oral dehydroepiandrosterone in elderly men: Significant increase in circulating estrogens.The Journal of Clinical Endocrinology & Metabolism,84(6),2170-2176.
2. Banfi, G.,Dolci, A.(2006).Free testosterone/cortisol ratio in soccer: Use fulness of a categorization of values.The Journal of Sports Medicine and Physical Fitness,46(4),611-616.
3. Barnhart, K. T.,Freeman, E.,Grisso, J. A.,Rader, D. J.,Sammel, M.,Kapoor, S.(1999).The effect of dehydroepiandrosterone supplementation to symptomatic perimenopausal women on serum endocrine profiles, lipid parameters, and health-related quality of life.The Journal of Clinical Endocrinology & Metabolism,84(11),3896-3902.
4. Bélanger, A.,Candas, B.,Dupont, A.,Cusan, L.,Diamond, P.,Gomez, J. L.(1994).Changes in serum concentrations of conjugated and unconjugated steroids in 40- to 80-year-old men.The Journal of Clinical Endocrinology & Metabolism,79(4),1086-1090.
5. Bonnefoy, M.,Kostka, T.,Patricot, M. C.,Berthouze, S. E.,Lacour, J. R.(2001).Lack of correlation between 6-month fluctuations in habitual physical activity and testosterone. Concentrations in elderly subjects.Journal of Sports Medicine and Physical Fitness,41(1),78-82.
6. Cumming, D. C.,Brunsting, L. A., III,Strich, G.,Ries, A. L.,Rebar, R. W.(1986).Reproductive hormone increases in response to acute exercise in men.Medicine & Science in Sports & Exercise,18(4),369-373.
7. Flynn, M. A.,Weaver-Osterholtz, D.,Sharpe-Timms, K. L.,Allen, S.,Krause, G.(1999).Dehydroepiandrosterone replacement in aging humans.The Journal of Clinical Endocrinology & Metabolism,84(5),1527-1533.
8. Guglielmini, C.,Paolini, A. R.,Conconi, F.(1984).Variations of serum testosterone concentrations after physical exercises of different duration.International Journal of Sports Medicine,5(5),246-249.
9. Hakkinen, K.,Kallinen, M.,Izquierdo, M.,Jokelainen, K.,Lassila, H.,Mälkiä, E.(1998).Changes in agonist-antagonist EMG, muscle CSA, and force during strength training in middle-aged and older people.Journal of Applied Physiology,84(4),1341-1349.
10. Harman, S. M.,Metter, E. J.,Tobin, J. D.,Pearson, J.,Blackman, M. R.(2001).Longitudinal effects of aging on serum total and free testosterone levels in healthy men.The Journal of Clinical Endocrinology & Metabolism,86(2),724-731.
11. Heck, R. W.,McKeever, K. H.,Alway, S. E.,Auge, W. K.,Whitehead, R.,Bertone, A. L.(1996).Resistance training-induced increases in muscle mass and performance in ponies.Medicine & Science in Sports & Exercise,28(7),877-883.
12. Jürimäe, J.,Jürimäe, T.,Pure, P.(2001).Plasma testosterone and cortisol responses to prolonged sculling in male competition rowers.Journal of Sports Sciences,19(11),893-898.
13. Kalimi, M.,Shafagoj, Y.,Loria, R.,Padgett, D.,Regelson, W.(1994).Anti-glucocorticoid effects of dehydroepiandrosterone (DHEA).Molecular and Cellular Biochemistry,131(2),99-104.
14. Kaufman, J. M.,Vermeulen, A.(2005).The decline of androgen levels in elderly men and its clinical and therapeutic implications.Endocrine Reviews,26(6),833-876.
15. Kawano, H.,Yasue, H.,Kitagawa, A.,Hirai, N.,Yoshida, T.,Soejima, H.(2003).Dehydroepiandrosterone supplementation improves endothelial function and insulin sensitivity in men.The Journal of Clinical Endocrinology & Metabolism,88(7),3190-3195.
16. Keller, C.,Keller, P.,Marshal, S.,Pedersen, B. K.(2003).IL-6 gene expression in human adipose tissue in response to exercise - Effect of carbohydrate ingestion.The Journal of Physiology,550,927-931.
17. Kroboth, P. D.,Salek, F. S.,Pittenger, A. L.,Fabian, T. J.,Frye, R. F.(1999).DHEA and DHEA-S: A review.The Journal of Clinical Pharmacology,39(4),327-348.
18. Labrie, F.,Bélanger, A.,Simard, J.,Luu-The, V.,Labrie, C.(1995).DHEA and peripheral androgen and estrogen formation: Intracrinology.Annals of the New York Academy of Sciences,774,16-28.
19. Labrie, F.,Diamond, P.,Cusan, L.,Gomez, J. L.,Bélanger, A.,Candas, B.(1997).Effect of 12-month dehydroepiandrosterone replacement therapy on bone, vagina, and endometrium in postmenopausal women.The Journal of Clinical Endocrinology & Metabolism,82(10),3498-3505.
20. Labrie, F.,Luu-The, V.,Labrie, C.,Simard, J.(2001).DHEA and its transformation into androgens and estrogens in peripheral target tissues: Intracrinology.Frontiers in Neuroendocrinology,22(3),185-212.
21. Lac, G.,Berthon, P.(2000).Changes in cortisol and testosterone levels and T/C ratio during an endurance competition and recovery.Journal of Sports Medicine and Physical Fitness,40(2),139-144.
22. Lin, H.,Wang, S. W.,Wang, R. Y.,Wang, P. S.(2001).Stimulatory effect of lactate on testosterone production by rat leydig cells.Journal of Cellular Biochemistry,83(1),147-154.
23. Liu, T. C.,Kuo, C. H.,Wang, P. S.(2009).Exercise and testosterone.Adaptive Medicine,1(1),26-31.
24. Lu, S. S.,Lau, C. P.,Tung, Y. F.,Huang, S. W.,Chen, Y. H.,Shih, H. C.(1997).Lactate and the effects of exercise on testosterone secretion: Evidence for the involvement of a cAMP-mediated mechanism.Medicine & Science in Sports & Exercise,29(8),1048-1054.
25. Lupien, S. J.,Schwartz, G.,Ng, Y. K.,Fiocco, A.,Wan, N.,Pruessner, J. C.(2005).The Douglas Hospital longitudinal study of normal and pathological aging: Summary of findings.Journal of Psychiatry & Neuroscience,30(5),328-334.
26. MacEwen, E. G.,Kurzman, I. D.(1991).Obesity in the dog: Role of the adrenal steroid dehydroepiandrosterone (DHEA).Journal of Nutrition,121(11),S51-S55.
27. Mäestu, J.,Jürimäe, J.,Jürimäe, T.(2005).Hormonal response to maximal rowing before and after heavy increase in training volume in highly trained male rowers.Journal of Sports Medicine and Physical Fitness,45(1),121-126.
28. Morales, A. J.,Haubrich, R. H.,Hwang, J. Y.,Asakura, H.,Yen, S. S.(1998).The effect of six months treatment with a 100 mg daily dose of dehydroepiandrosterone (DHEA) on circulating sex steroids, body composition and muscle strength in age-advanced men and women.Clinical Endocrinology,49(4),421-432.
29. Mulligan, T.,Iranmanesh, A.,Johnson, M. L.,Straume, M.,Veldhuis, J. D.(1997).Aging alters feed-forward and feedback linkages between LH and testosterone in healthy men.The American Journal of Physiology-Regulatory, Integrative and Comparative Physiology,273(2),R1407-R1413.
30. Nair, K. S.,Rizza, R. A.,O'Brien, P.,Dhatariya, K.,Short, K. R.,Nehra, A.(2006).DHEA in elderly women and DHEA or testosterone in elderly men.The New England Journal of Medicine,355,1647-1659.
31. Nestler, J. E.,Barlascini, C. O.,Clore, J. N.,Blackard, W. G.(1988).Dehydroepiandrosterone reduces serum low density lipoprotein levels and body fat but does not alter insulin sensitivity in normal men.The Journal of Clinical Endocrinology & Metabolism,66(1),57-61.
32. Nindl, B. C.,Kraemer, W. J.,Deaver, D. R.,Peters, J. L.,Marx, J. O.,Heckman, J. T.(2001).LH secretion and testosterone concentrations are blunted after resistance exercise in men.Journal of Applied Physiology,91(3),1251-1258.
33. Orentreich, N.,Brind, J. L.,Rizer, R. L.,Vogelman, J. H.(1984).Age changes and sex differences in serum dehydroepiandrosterone sulfate concentrations throughout adulthood.The Journal of Clinical Endocrinology & Metabolism,59(3),551-555.
34. Prioux, J.,Ramonatxo, M.,Hayot, M.,Mucci, P.,Préfaut, C.(2000).Effect of ageing on the ventilatory response and lactate kinetics during incremental exercise in man.European Journal of Applied Physiology,81(1-2),100-107.
35. Roth, G. S.,Lane, M. A.,Ingram, D. K.,Mattison, J. A.,Elahi, D.,Tobin, J. D.(2002).Biomarkers of caloric restriction may predict longevity in humans.Science,297(5582),811.
36. Rudman, D.,Shetty, K. R.,Mattson, D. E.(1990).Plasma dehydroepiandrosterone sulfate in nursing home men.Journal of the American Geriatrics Society,38(4),421-427.
37. Sato, K.,Iemitsu, M.,Aizawa, M.,Ajisaka, R.(2008).Testosterone and DHEA activate the glucose metabolism-related signaling pathway in skeletal muscle.American Journal of Physiology Endocrinology and Metabolism,294(5),E961-E968.
38. Straub, R. H.,Konecna, L.,Hrach, S.,Rothe, G.,Kreutz, M.,Schölmerich, J.(1998).Serum dehydroepiandrosterone (DHEA) and DHEA sulfate are negatively correlated with serum interleukin-6 (IL-6), and DHEA inhibits IL-6 secretion from mononuclear cells in man in vitro: Possible link between endocrinosenescence and immunosenescence.The Journal of Clinical Endocrinology & Metabolism,83(6),2012-2017.
39. Tchernof, A.,Després, J. P.,Bélanger, A.,Dupont, A.,Prud'homme, D.,Moorjani, S.(1995).Reduced testosterone and adrenal C19 steroid levels in obese men.Metabolism,44(4),513-519.
40. Villareal, D. T.,Holloszy, J. O.,Kohrt, W. M.(2000).Effects of DHEA replacement on bone mineral density and body composition in elderly women and men.Clinical Endocrinology,53(5),561-568.
41. Yeap, B. B.,Almeida, O. P.,Hyde, Z.,Norman, P. E.,Chubb, S. A. P.,Jamrozik, K.(2007).In men older than 70 years, total testosterone remains stable while free testosterone declines with ageage. The health in men study.European Journal of Endocrinology,156(5),585-594.