黑大蒜補充對高中長跑選手抗氧化與抗發炎之影響

Effects of Black Garlic Supplementation on Antioxidant and Anti-Inflammatory Responses in Senior High School Long-Distance Runners

10.5297/ser.1904.007

傅正思(Cheng-Sze Fu)；李淑玲(Shu-Lin Lee)

超氧化歧化酶 ； 總抗氧化狀態 ； 高敏感性C－反應蛋白 ； superoxide dismutase ； total antioxidant status ； high-sensitivity C-reactive protein

大專體育學刊

19卷4期（2017 / 12 / 31）

384 - 395

繁體中文;英文

本研究目的主要探討黑大蒜補充對長跑選手抗氧化與抗發炎之影響。以7位高中長跑選手為本實驗之受試對象，雙盲交叉設計分別進行14天黑大蒜或安慰劑補充，並在第15天進行一萬公尺運動測試，於補充前與運動後，採集血液並進行超氧化歧化酶(SOD)、總抗氧化狀態(TAS)、麩胱甘肽過氧化酶(GPx)等氧化能力指標，與肌酸激酶、乳酸脫氫酶、高敏感性C－反應蛋白、白血球數(WBC)等發炎指標分析。研究結果顯示：在一萬公尺運動測試後黑大蒜組體內抗氧化參數顯著低於安慰劑組，發炎情形中白血球數指標在黑大蒜組中顯著低於安慰劑組。本研究結論：黑大蒜補充可以顯著降低抗氧化指標SOD、TAS與GPx活性及白血球數量，有效維持體內之抗氧化能力及降低運動後發炎情形。

The purpose of this study was to examine the effects of black garlic supplementation on antioxidant and anti-inflammatory status in senior high school long distance runners. Seven long-distance runners were randomly allocated into black garlic group or placebo group. After fourteen days supplementation, blood samples were collected to determine the pre-supplement intervention and post-ten thousand meters exercise testing effect in both groups. Blood sample were analyzed to determine the superoxide dismutase (SOD), total antioxidant status (TAS) and glutathione peroxidase (GPx) as index of antioxidant capacity. Besides, creatine kinase level (CK), high-sensitivity C-reactive protein (hs-CRP) and white blood cell (WBC) count were assessed as inflammatory markers. The results indicated that SOD, TAS, GPx and WBC were significantly lower in black garlic group than in placebo group. In conclusion, black garlic supplementation can effectively improve the antioxidant status and decrease the WBC count in long-distance runners.

1. 戴堯種、林正常(2003)。低水溫游泳訓練對抗氧化酵素之相關研究。大專體育學刊，5(1)，239-248。
   連結：
2. Amagase, H.,Petesch, B. L.,Matsuura, H.,Kasuga, S.,Itakura, Y.(2001).Intake of garlic and its bioactive components.The Journal of Nutrition,131(3),955S-962S.
3. Antonio, J.,Sanders, M. S.,Ehler, L. A.,Uelmen, J.,Raether, J. B.,Stout, J. R.(2000).Effects of exercise training and amino-acid supplementation on body composition and physical performance in untrained women.Nutrition,16(11-12),1043-1046.
4. Aoi, W.,Naito, Y.,Takanami, Y.,Kawai, Y.,Sakuma, K.,Ichikawa, H.,Yoshikawa, T.(2004).Oxidative stress and delayed-onset muscle damage after exercise.Free Radical Biology & Medicine,37(4),480-487.
5. Barnes, P. J.,Stockley, R. A.(2005).COPD: Current therapeutic interventions and future approaches.European Respiratory Journal,25(6),1084-1106.
6. Borek, C.(2001).Antioxidant health effects of aged garlic extract.The Journal of Nutrition,131(3),1010S-1015S.
7. Colín-González, A. L.,Santana, R. A.,Silva-Islas, C. A.,Chánez-Cárdenas, M. E.,Santamaría, A.,Maldonado, P. D.(2012).The antioxidant mechanisms underlying the aged garlic extractand S-allylcysteine-induced protection.Oxidative Medicine and Cellular Longevity,2012
8. Han, C. H.,Liu, J. C.,Chen, K. H.,Lin, Y. S.,Chen, C. T.,Fan, C. T.,Hou, W. C.(2011).Antihypertensive activities of processed garlic on spontaneously hypertensive rats and hypertensive humans.Botanical Studies,52(3),277-283.
9. Ide, N.,Lau, B. H. S.(2001).Garlic compounds minimize intracellular oxidative stress and inhibit nuclear factor-κB activation.The Journal of Nutrition,131(3),1020S-1026S.
10. Jeong, Y. Y.,Ryu, J. H.,Shin, J. H.,Kang, M. J.,Kang, J. R.,Han, J.,Kang, D.(2016).Comparison of anti-oxidant and anti-inflammatory effects between fresh and aged black garlic extracts.Molecules,21(4),430.
11. Ji, L. L.,Gomez-Cabrera, M. C.,Vina, J.(2006).Exercise and hormesis: Activation of cellular antioxidant signaling pathway.Annals of the New York Academy of Sciences,1067,425-435.
12. Jung, Y. M.,Lee, S. H.,Lee, D. S.,You, M. J.,Chung, I. K.,Cheon, W. H.,Ku, S. K.(2011).Fermented garlic protects diabetic, obese mice when fed a high-fat diet by antioxidant effects.Nutrition Research,31(5),387-396.
13. Kim, H. K.,Choi, Y. W.,Lee, E. N.,Park, J. K.,Kim, S. G.,Park, D. J.,Yoon, S.(2011).5-Hydroxymethylfurfural from black garlic extract prevents TNFα-induced monocytic cell adhesion to HUVECs by suppression of vascular cell adhesion molecule-1 expression, reactive oxygen species generation and NF-κB activation.Phytotherapy Research,25(7),965-974.
14. Kim, K. M.,Chun, S. B.,Koo, M. S.,Choi, W. J.,Kim, T. W.,Kwon, Y. G.,Kim, Y. M.(2001).Differential regulation of NO availability from macrophages and endothelial cells by the garlic component S-allyl cysteine.Free Radical Biology & Medicine,30(7),747-756.
15. Lawson, L. D.(Ed.),Koch, H. P.(Ed.)(1996).Garlic: The science and therapeutic applications of Allium sativum L. and related species.Baltimore, UK:Williams & Wilkins.
16. Lee, Y. M.,Gweon, O. C.,Seo, Y. J.,Im, J.,Kang, M. J.,Kim, M. J.,Kim, J. I.(2009).Antioxidant effect of garlic and aged black garlic in animal model of type 2 diabetes mellitus.Nutrition Research and Practice,3(2),156-161.
17. Morihara, N.,Hayama, M.,Fujii, H.(2011).Aged garlic extract scavenges superoxide radicals.Plant Foods for Human Nutrition,66(1),17-21.
18. Mourkioti, F.,Rosenthal, N.(2008).NF-κB signaling in skeletal muscle: Prospects for intervention in muscle diseases.Journal of Molecular Medicine,86(7),747-759.
19. Park, J.H.,Park, Y.K.,Park, E.(2009).Antioxidative and antigenotoxic effects of garlic (Allium sativum L.) prepared by different processing methods.Plant Foods for Human Nutrition,64(4),244-249.
20. Peterson, J. M.,Guttridge, D. C.(2008).Skeletal muscle diseases, inflammation, and NF-κB signaling: Insights and opportunities for therapeutic intervention.International Reviews of Immunology,27(5),375-387.
21. Seo, D. Y.,Lee, S. R.,Kim, H. K.,Baek, Y. H.,Kwak, Y. S.,Ko, T. H.,Han, J.(2012).Independent beneficial effects of aged garlic extract intake with regular exercise on cardiovascular risk in postmenopausal women.Nutrition Research and Practice,6(3),226-231.
22. Williams, M. J.,Sutherland, W. H.,McCormick, M. P.,Yeoman, D. J.,de Jong, S. A.(2005).Aged garlic extract improves endothelial function in men with coronary artery disease.Phytotherapy Research,19(4),314-319.