Quantitative Determination of Artificial Antioxidants in Foods Using Nanoparticles Modified Glassy Carbon Electrodes

10.6578/TJACFS.202406_62(2).0002

Yi-Ming Huang；Yu-Hua Chen；Chuan-Liang Hsu

Nanoparticles modified glassy carbon electrode ； BHA ； BHT ； TBHQ ； Electroanalytical method ； Antioxidant

臺灣農業化學與食品科學

62卷2期（2024 / 06 / 01）

63 - 74

英文

A simple voltammetric method, employing Au nanoparticles modified glassy carbon electrode (AuNPs/ GCE), was developed for the determination of butylated hydroxylanisole (BHA), butylated hydroxytoluene (BHT), and tert-butylhydroquinone (TBHQ), the widely used synthetic phenolic antioxidants in foods. As a result, the electrochemical behavior of the antioxidants could be simultaneously detected only at the AuNPs/ GCE. The AuNPs/GCE allowed the detection of the three analytes at potentials lower than those observed at bare GCE. The stability and repeatability of the AuNPs/GCE were also confirmed in the study. Under the optimized conditions, the method presented a linear response in the concentration range of 0~330 mg/ L for TBHQ (R^2 = 0.9962), BHA (R^2 = 0.9974) and BHT (R^2 = 0.9968), respectively. The proposed method was effectively utilized to simultaneously quantify BHA, BHT, and TBHQ in various edible oil products. The obtained results were found to be consistent with those obtained through the high-performance liquid chromatography (HPLC) method. The analytical utility of the AuNPs/GCE-based sensor was evaluated through a recovery study, achieving an average recovery of 95.56% to 104.98% for the three antioxidants. These results indicated satisfactory precision and accuracy, suggesting the potential use of the developed electroanalytical method for detecting these antioxidants in food products.