微脂粒添加劑對安定性之探討

Investigation of Stability Effect of Additives on Liposomes

10.30001/JIES.201103.0004

陳崇裕(Chon-Yu Chen)；陳麗舟(Li-Chou Chen)；廖湘陵(Hsiang-Ling Liao)

微脂粒 ； r-PGA ； 乙醇注射法 ； 超音波震 ； □法 ； Liposome ； stability ； Arbutin ； Antioxdants

美容科技學刊

8卷1期（2011 / 03 / 01）

41 - 51

繁體中文

本研究以薄膜水合法／超音波震盪法，以功率33W/40mins製備熊果素微脂粒添加不同抗氧化劑配方，並進行安定性評估及安全性。實驗結果，以C組配方(PC: Arbutin: Tocopherol/3:0.5:0.5)平均粒徑最大，約109nm~117nm；D組配方(PC: Arbutin: Tocopherol: AC Leucidal/3:0.5:0:1)所得粒徑最小，其平均粒徑為26nm~29nm。在安定性評估，以E組配方(PC: Arbutin: Tocopherol: AC Leucidal/3:0.5:0.5:D，儲存於不同溫度下7~28天後，粒徑範圍在109nm~121nm之間，變化較小較為穩定。熊果素微脂粒配方進行24 Hrs皮膚過敏性測試，並無過敏現象產生。

This research used thin film hydration/sonication equipment with power 33W/40 mins to prepare arbutin liposome added with different antioxidant additives. The products also do efficacy and safety test. The results showed formulation C (PC: Arbutin: Tocopherol: AC Leucidal/3:0.5:0:1) with largest average particle size about 109 nm~117 nm and formulation D (PC: Arbutin: Tocopherol: AC Leucidai/3:0.5:0:1) with smallest average particle size about 26 nm~29 nm. Concerning the stability test, formulation E (PC: Arbutin: Tocopherol: AC Leucidal/3:0.5:0.5:1) expressed the best re-suits with average particle size about 109 nm~121 nm after 7~28 days storage under different temperature. The arbutin liposome products also showed no allergy results after 24 hrs skin test.

1. (2007).,Datamonitor Corporation.
2. OECD, Organisation for Economic Co-operation and Development. Guideline for Testing of Chemicals. 1992. No. 404: Acute Dermal Irritation/Corrosion..
3. Collins J. J.,Phillips, M.(1982).The Stability and Structure ofn Cholesterol-rich Codispersions of Cholesterol abd Phosphatidylcholine.J. Lipid Res.,23,291-298.
4. Frezard F.,Santaella, C.,Vierling, P.,Riess, J. G.(1994).Fluorinated-Based Vesicles as Potential Drug Carriers: Encapsulation/ Sustaining of Drugs and Stability in Human Serum.Biomater. Artif. Cells. Immobil. Biotechnol.,22,1403-1408.
5. Lasic, Danilo D.,Needham, D.(1995).The Stealth Liposome: A Prototypical Biomaterial.Chem. Rev.,95,2601-2628.
6. Sugimoto, K.(2003).Syntheses of arbutin-alpha-glycosides and a comparison of their inhibitory effects with those of alpha-arbutin and arbutin on human tyrosinase.Chem Pharm Bull (Tokyo),51(7),798-801.
7. Suzuki, K.,Sakon, K.(1990).The application of liposomes to coseetics.Cosmetic & Toiletries,105,65-77.
8. Tagawa, M.,Shinozaki, K.,Kurata, Y.,Matsumoto, K.,Tabata, Y.(1986).Applications of hydrogenated lecithin for cosmetics.14 th IFSCC,1,335.
9. Trevino L.,Frezard, F.,Roll, J. P.,Postel, M.,Riess, J. G.(1994).Novell Liposome Systems Based on the Incorporation of (Perfluoroalkyl) alkenes(Fm H n E) into the Bilayer of Phospholipid Liposome.Colloids Surf,88,23-233.
10. Woodle M. C.,Newman, M. S.,Cohen, J. A.(1994).Sterically Stabilized Liposome: Physical and Biological Properties.J. Drug. Target.,2,397-403.
11. 劉得任(2000)。博士論文(博士論文)。國立中央大學化學工程研究所。
12. 蕭伊妙(2007)。碩士論文(碩士論文)。國立清華大學化學工程學系。