具有去除染髮劑色素功能之土壤細菌篩選與鑑定

Isolation and Identification of Hair Dye Decolorization Soil Bacteria

10.30001/JIES.201209.0003

蕭蓉禎(Rong-Jen Shiau)

染髮劑色素 ； 去除色素 ； 土壤細菌 ； 增殖培養 ； Hair dye ； Decolorization ； Soil bacteria ； Enrichment culture

美容科技學刊

9卷3期（2012 / 09 / 01）

25 - 36

繁體中文

隨著國人生活水準提昇，美容、美髮業發展迅速，美容美髮各項產品使用量大幅提高。然而，大量使用美妝產品（例如：染髮劑等），其污水若未經適當處理，則會對環境造成極大的危害。本研究旨在篩選具有去除染髮劑色素功能之土壤細菌。利用市售染髮劑做為基質，以增殖培養法（enrichment culture）篩選土壤細菌，獲得B16以及B21二株具有移除溶液中染髮劑色素能力的細菌。將菌株的16S rRNA基因定序後，與基因庫中的已知菌株的16S rRNA基因序列進行比對，結果顯示B16以及B21與Pseudomonas putida非常相近。本研究所分離的菌株未來可應用於美髮業污水處理，減少美髮業污水對環境的危害。

As raising the level of life, beauty and hairdressing industries are developing rapidly. However, the waste water of beauty products (such as hair dyes) might harm to the environment. The purpose of this study was to discover soil bacteria which can remove hair dye pigments from aquatic solutions. Using a commercial hair dye as matrix, the enrichment culture method was applied to obtain bacteria. Two bacterial strains were isolated, named B16 and B21. The sequences of 16S rRNA of these two bacteria were submitted to Genbank for species identification. The result showed that both B16 and B21 were highly similar to Pseudomonas putida. These two bacteria could be used for removing hair dye pigments for the hairdressing industry.

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