臺灣羅勒葉粉末抗發炎之研究

Study on Anti-Inflammation of Taiwan Ocimum basilicum L. Leaf Powder

10.30001/JIES.202212_19(2).0003

林文宏(Lin, Wen-Hung)；劉家祐(Liu, Chia-Yu)；王郁玟(Wang, Yu-Wen)；郭恆宏(Kuo, Heng-Hung)

羅勒 ； 發炎因子 ； 巨噬細胞 ； Ocimum basilicum L ； inflammatory factors ； macrophage

美容科技學刊

19卷2期（2022 / 12 / 01）

41 - 54

繁體中文

本文以臺灣羅勒（Ocimum basilicum L.）俗稱九層塔的植物葉部做為材料，研究其對巨噬細胞株的抗發炎效果。體外培養的細胞株被應用來分析下列三種促炎因子，包含一氧化氮（nitric oxide, NO）、白介素6（interleukin 6, IL-6）及腫瘤壞死因子－α（tumor necrosis factor-alpha, TNF-α）；並進行細胞毒性（microculture tetrazolium assay, MTT assay）分析。結果顯示，羅勒葉並不具抑制由細菌脂多醣（lipopolysaccharide, LPS）所誘導巨噬細胞株產生的NO、IL-6與TNF-α之發炎反應。同時，在本研究之測試濃度下，未測得顯著細胞毒性。綜上所述，羅勒葉在體外培養的細胞模式中，不能有效抑制LPS所刺激誘導NO、IL-6與TNF-α的發炎反應，且無毒性反應。

In this research, the leaves of Taiwan Ocimum basilicum L. were used as materials to explore its anti-inflammatory effect on macrophage cell lines. In vitro cultured cell lines were used to analyze the following three pro-inflammatory factors, including nitric oxide (NO), interleukin 6 (IL-6) and tumor necrosis factor-α (TNF -α); In addition, cytotoxicity (microculture tetrazolium assay, MTT assay) analysis was performed. The results showed that Ocimum basilicum L. leaves did not inhibit the inflammatory response of NO, IL-6 and TNF-α produced by bacterial lipopolysaccharide (LPS)-induced macrophage cell lines. Meanwhile, no significant cytotoxicity was detected at the concentrations tested in this study. In conclusion, Ocimum basilicum L, leaves cannot effectively inhibit the inflammatory response of NO, IL-6 and TNF-α induced by LPS stimulation in the cell model of in vitro culture, and there is no toxic reaction.

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