腺苷酸抑制虹彩病毒在石斑魚腎臟細胞複製之研究

A Study of Adenosine-Mediated Inhibition the Replication of Iridovirus in Grouper Kidney Cells

10.29855/JCTCN.201210.0002

林淑玟(Sui-Wen Lin)；黃建智(Jian-Jhih Huang)；賴裕順(Yu-Shen Lai)

腺苷酸 ； 虹彩病毒 ； adenosine ； iridovirus

耕莘學報

10期（2012 / 10 / 01）

15 - 24

繁體中文

腺苷酸是屬於一種嘌呤類核苷，其結構是由含氮鹽基與五碳糖組成。在先前的研究中指出，腺苷酸能有效的抑制牛痘病毒的複製，因此在本研究中，將要探討腺苷酸是否能抑制石斑魚虹彩病毒在石斑魚腎臟細胞中的複製，並進一步探討其影響機制。研究結果發現石斑魚腎臟細胞事先處理腺苷酸之後，虹彩病毒力價顯著低於沒有處理的組別，隨著腺苷酸濃度的提高，對病毒增殖抑制的情形也隨之提高。接著利用RT-PCR技術來檢測虹彩病毒的早、晚期基因表現，結果發現腺苷酸會抑制虹彩病毒感染複製，並且延遲病毒早、晚基因的表現。

Adenosine is a purine nucleoside comprising a molecule of adenine attached to a ribose sugar molecule (ribofuranose) moiety via a β-N9-glycosidic bond. It has been previously demonstrated that adenosine can inhibit the replication of vaccinia virus in the monkey kidney BSC-40 cells. In this study, we investigated whether adenosine can interfere with the replication of grouper iridovirus (GIV) in grouper kidney (GK) cells. Our datas showed that the replication of GIV was inhibited by adenosine in GK cells. In RT-PCR assay, we found that the pre-treatment with adenosine resulted in a delayed expression of the early and late genes of GIV in the GK cells.