Component Analysis of Isoflavonoids in Pueraria montana and Pueraria lobata by High Performance Liquid Chromatography

使用高效液相層析法分析臺灣葛藤及大葛藤之異黃酮類成分

10.30061/CEB.200506.0003

楊千瑩(Chian-Ying Yang)；陳建志(Chien-Chih Chen)；林素如(Su-Ju Lin)；陳嘉琪(Chia-Chi Chen)；賴宏亮(Horng-Liang Lay)

葛藤 ； 大葛藤 ； 臺灣葛藤 ； 葛根素 ； 大豆苷 ； 染料木苷 ； 染料木素 ； 大豆苷元 ； 大豆苷元4',7二葡萄苷 ； Puerariae radix ； P. Montana ； P. lobata ； Puerarin ； Daidzin ； Genistin ； Genistein ； Daidzein ； Daidzein-4',7-diglucoside

作物、環境與生物資訊

2卷2期（2005 / 06 / 01）

115 - 122

英文

葛藤(Pueraria spp.)之根部，為傳統常用之中藥材。分佈於臺灣之葛藤品種主要有大葛藤(P. lobata)及臺灣葛藤(P. montana)等兩種。葛根中含多種異黃酮，包括puerarin, daidzin, genistin, genistein, daidzein及daidzein-4',7-diglucoside等。本研究以六種異黃酮成分為指標成分開發多成分同時分析之HPLC方法，並進行葛藤二種不同物種及不同部位（根、藤、葉及葉柄）之定量分析，探討其品質之差異性。結果顯示黃酮類含量以大葛藤(P. lobata)根及藤為最高，而daidzein-4',7-diglucoside僅存在於大葛藤之根及藤中，各成分之含量在各部位及種間皆有明顯差異。

Radix of Pueraria spp. is a popular traditional Chinese medicine. There are two major species of Pueraria in Taiwan, i.e., Pueraria montana and Pueraria lobata. The major constituents of Puerariae radix were isoflavonoids, which include puerarin, daidzin, genistin, genistein, daidzein and daidzein-4', 7- diglucoside. This study developed simultaneous analysis of multi-component HPLC method to determine the six components of isoflavonoids in root, vine, leaf, and petiole parts of P. montana and P. lobata for establishing a standard quality control of Pueraria spp. Results showed that the highest level of isoflavonoids was observed in the root and vine of P. lobata, and the daidzein-4', 7-diglucoside was only found in root and vine of P. lobata. Levels of isoflavones existed among species, as well as organs, were significantly different.