题名 |
利用型態及生理指標評估甘藷誘變品系之苗期耐旱性 |
并列篇名 |
Evaluation on Drought Tolerance of Sweetpotato Mutants at Seedling Stage with Morphological and Physiological Indicators |
DOI |
10.30061/CEB.202312_19.0006 |
作者 |
陸柏憲(Po-Hsien Lu);侯惠盈(Hui-Ying Hou);黃哲倫(Che-Lun Huang);葉亦琪(I-Chi Yeh);王強生(Chang-Sheng Wang);陳甘澍(Kan-Shu Chen);賴永昌(Yung-Chang Lai) |
关键词 |
甘藷 ; 誘變品系 ; 耐旱性 ; 逆境指標 ; 隸屬函數 ; Sweetpotato ; Mutants ; Drought tolerance ; Stress indicators ; Membership function |
期刊名称 |
作物、環境與生物資訊 |
卷期/出版年月 |
19卷(2023 / 12 / 31) |
页次 |
69 - 84 |
内容语文 |
繁體中文;英文 |
中文摘要 |
本試驗利用11個甘藷誘變品系及3個對照品種(TN57、TN66、TN73)為材料,調查乾旱逆境下植株之型態及生理指標,評估不同甘藷基因型之苗期耐旱性。型態指標之試驗結果顯示,與對照組相比,乾旱處理組之葉片數顯著減少66.8%、莖鮮重下降49.4%、總鮮重下降42.3%,而莖長、莖寬及根鮮重則無顯著差異。其次,以變異係數最大之葉片數性狀為篩選指標,初步選出7個甘藷品系(種)進行下一步葉片生理指標分析。經複因子變方分析結果顯示,葉溫及丙二醛(malondialdehyde, MDA)含量指標呈顯著交感效應,表示乾旱逆境下兩生理指標之變化因不同甘藷基因型而異,可作為評估甘藷耐旱性之良好逆境指標;此外,乾旱效應顯著影響葉片SPAD、葉綠素a、b及總葉綠素含量,而對類胡蘿蔔素含量及葉綠素a/b無顯著影響。最終,本試驗將型態及生理指標數據代入耐旱係數公式(yield stability index, YSI)並進行隸屬函數分析,結果顯示以M29誘變品系具有最佳之苗期綜合耐旱性,其次為M33和M27品系。本試驗之耐旱篩選指標及耐旱性評估結果可供未來甘藷乾旱逆境生理及耐旱育種研發之參考。 |
英文摘要 |
This study investigated the morphological and physiological responses of 11 sweetpotato mutants and 3 check varieties (TN57, TN66, TN73) under drought stress, and aimed to evaluate the drought tolerance of sweetpotato genotypes at the seedling stage. The results showed that the number of leaves in the drought-treated group decreased by 66.8%, stem fresh weight decreased by 49.4%, and total fresh weight decreased by 42.3%, compared to the control group. However, there were no significant differences in stem length, stem width, and root fresh weight between the CK and drought-treated group. Furthermore, using leaf number, which had the highest coefficient of variation, as a screening criterion, seven sweetpotato genotypes were preliminarily selected for further analysis of leaf physiological indicators. The results of two-way ANOVA indicated that leaf temperature and malondialdehyde (MDA) content indicators showed significant interaction effects, and it suggested that the response to drought stress varied among different sweetpotato genotypes. Therefore, leaf temperature and MDA could serve as reliable and effective indicators for drought tolerance evaluation. In addition, the drought stress significantly affected leaf SPAD, chlorophyll a, chlorophyll b and total chlorophyll contents, while there were no significant changes in carotenoid content and chlorophyll a/b ratio under drought stress. Overall, the comprehensive evaluation of drought tolerance by using the stress index formula (yield stability index, YSI) and membership function indicated that the M29 mutant exhibited the highest drought tolerance at the seedling growth stage, followed by the M33 and M27. The results of this study could be used as a reference for future research on the physiology of drought stress and drought-tolerant breeding for sweetpotato. |
主题分类 |
生物農學 >
生物農學綜合 生物農學 > 農業 生物農學 > 森林 生物農學 > 畜牧 生物農學 > 漁業 |