The Cloning of Water-Deficit-Stress Induced lp3 Gene from Loblolly Pine

乾旱誘導德達松lp3基因之克隆

10.29950/JHKUM.200607.0005

王昭泰(Jau-Tay Wang)

水分逆境 ； 轉殖 ； 體染色體克隆 ； 南方點漬法 ； water deficit stress (WDS) ； transformation ； Genomic clone ； Southern blot hybridization

興國學報

6期（2006 / 07 / 01）

69 - 92

英文

水分逆境（WDS）是影響德達松生存與生長之主要因素之一。分離與克隆乾旱誘導基因是解開基因如何運作的謎團之重要一環。許多德達松基因已經被克隆了（lp1, lp2, lp3, lp4, lp5, lp8, lp15, lp20）。其中一個基因－lp3，引起我的注意。預測性的LP3蛋白與ASR（離層酸ABA，逆境Stress與果時成熟Ripening）蛋白相似。在C端相似度高達85%，然而在N端有所差異。因為lp3基因編碼的是親水性蛋白，可能是細胞核內基因，它的功能可能是在乾旱逆境時細胞核之保護物，可能受到離層酸或無離層酸之影響；抑或為轉錄起始物。在兩種情況下，此蛋白為核內基因。此研究幫助我們了解德達松乾旱誘導反應，並建立長遠的目標－改造樹木基因，增加乾旱的耐受能力。

Water deficit stress (WDS) is one of the most important factors affecting survival and growth of loblolly pine (Pinus taeda). The isolation and cloning of genes which are induced by WDS is the keystone to unveil the puzzle how the genes work. Many of cDNA (lp1, lp2, lp3, lp3, lp4, lp5 and lp8) had been isolated. One of these genes is catching my eyes is lp3 gene. Putative Lp3 protein is similar to ASR (ABA, Stress and Ripening　protein). In the C-terminal region, the homology is very high up to 85%, the sequence is variable at N-terminal region. Since lp3 codes for a highly hydrophilic protein with a putative nuclear targeting sequence within it, we conclude its function either as a protectant in the nucleus during WDS with or without the involvement of ABA, or it may act as a transcriptional activator. In either case, it is a nuclear binding protein. This study is helping us to understand the WDS-response in loblolly pine in achieving our long term goal of genetically engineered tree species for increased WDS tolerance.

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