運動預處理可能減少血漿對離體紅血球脂質氧化作用

Exhausted Exercise Preconditioning May Cause Plasma to Prevent Lipid Peroxidation of RBC in Vitro

10.6634/JPSS-CCU.201306.16.06

沙部．魯比(Sha Pu Lu Bi)；林嘉志(Chia-Chih Lin)；楊忠祥(Chung-Hsiang Yang)

氧化傷害 ； 抗氧化酶活性 ； 尿酸 ； oxidative damage ； antioxidant enzyme activity ； uric acid

文化體育學刊

16輯（2013 / 06 / 01）

53 - 68

繁體中文

Purpose: Exercise preconditioning has been known to reduce strenuous exercise-induced oxidative damages. To understand the underlining mechanisms, plasma samples isolated from exhausted exercises were used to investigate the possible protection effect on RBC with or without following hypoxia/reoxygenation. Methods: Eight healthy male participants were recruited voluntarily (age: 28.9 ± 8.3 yr, height: 170.1 ± 5.9 cm, weight: 68.9 ± 7.4 kg). Blood samples were collected before (resting, r) and after (post exercise, e) running by Bruce graded exercise testing (GXT). Plasma and RBC were separated and then recombined again into 4 groups: rWB (rWhole Blood), rRrP (rRBC + rPlasma), rReP (rRBC + ePlasma), rRs (rRBC + PBS). All groups were then treated with hypobaric hypoxia at PO2 = 45 mmHg (H/N) or normbaric normoxia at PO2 = 152 mmHg (N/N) for 1 hr and then recovered by N/N for 5 min. Data were to compare differences among the groups at indicators including antioxidant enzyme activities, peroxidized lipid (TBARS), lactate, glucose and uric acid. Results: As compared to the control group, plasma with exercise preconditioning had significantly increased levels of lactate (15.26 ± 0.73 mM vs. 2.88 ± 0.19 mM, p ＜ .05), glucose (120.18 ± 6.40 mg．dL^(-1) vs. 98.12 ± 5.09 mg．dL^(-1), p ＜. 05) and uric acid (5.64 ± 0.53 mg．dL^(-1) vs. 4.56 ± 0.48 mg．dL^(-1), p ＜ .05). Except for eReP, RBC's TBARS level was significantly elevated in rWB, rRrP, rRs after N/N treatment as compared to rP (146.35 ± 32.48 mnol．gHb^(-1), 141.97 ± 34.4 mnol．gHb^(-1), 148.42 ± 29.8 mnol．gHb^(-1) vs. 59.72 ± 16.31 mnol．gHb^(-1), ＜ .05) or eP along (146.35 ± 32.48 mnol．gHb^(-1), 141.97 ± 34.4 mnol．gHb^(-1), 148.42 ± 29.8 mnol．gHb^(-1), vs. 50.53 ± 21.33 mnol．gHb^(-1), p＜ .05). However, this protection effect contributed to eP cannot be observed in groups after H/ treatment. All antioxidant enzyme activities among the groups were not different in both N/N and H/N treatments. Conclusion: Plasma with exhausted exercise preconditioning may reduce RBC peroxidized lipid damages but may not in hypoxia and reoxygenation. Further studies are needed to explore the role of uric acid in this reaction.

Purpose: Exercise preconditioning has been known to reduce strenuous exercise-induced oxidative damages. To understand the underlining mechanisms, plasma samples isolated from exhausted exercises were used to investigate the possible protection effect on RBC with or without following hypoxia/reoxygenation. Methods: Eight healthy male participants were recruited voluntarily (age: 28.9 ± 8.3 yr, height: 170.1 ± 5.9 cm, weight: 68.9 ± 7.4 kg). Blood samples were collected before (resting, r) and after (post exercise, e) running by Bruce graded exercise testing (GXT). Plasma and RBC were separated and then recombined again into 4 groups: rWB (rWhole Blood), rRrP (rRBC + rPlasma), rReP (rRBC + ePlasma), rRs (rRBC + PBS). All groups were then treated with hypobaric hypoxia at PO2 = 45 mmHg (H/N) or normbaric normoxia at PO2 = 152 mmHg (N/N) for 1 hr and then recovered by N/N for 5 min. Data were to compare differences among the groups at indicators including antioxidant enzyme activities, peroxidized lipid (TBARS), lactate, glucose and uric acid. Results: As compared to the control group, plasma with exercise preconditioning had significantly increased levels of lactate (15.26 ± 0.73 mM vs. 2.88 ± 0.19 mM, p ＜ .05), glucose (120.18 ± 6.40 mg．dL^(-1) vs. 98.12 ± 5.09 mg．dL^(-1), p ＜. 05) and uric acid (5.64 ± 0.53 mg．dL^(-1) vs. 4.56 ± 0.48 mg．dL^(-1), p ＜ .05). Except for eReP, RBC's TBARS level was significantly elevated in rWB, rRrP, rRs after N/N treatment as compared to rP (146.35 ± 32.48 mnol．gHb^(-1), 141.97 ± 34.4 mnol．gHb^(-1), 148.42 ± 29.8 mnol．gHb^(-1) vs. 59.72 ± 16.31 mnol．gHb^(-1), ＜ .05) or eP along (146.35 ± 32.48 mnol．gHb^(-1), 141.97 ± 34.4 mnol．gHb^(-1), 148.42 ± 29.8 mnol．gHb^(-1), vs. 50.53 ± 21.33 mnol．gHb^(-1), p＜ .05). However, this protection effect contributed to eP cannot be observed in groups after H/ treatment. All antioxidant enzyme activities among the groups were not different in both N/N and H/N treatments. Conclusion: Plasma with exhausted exercise preconditioning may reduce RBC peroxidized lipid damages but may not in hypoxia and reoxygenation. Further studies are needed to explore the role of uric acid in this reaction.

1. 沙部．魯比、周峻忠、林正常、林嘉志(2011)。肌力與耐力跑訓練順序對能量代謝產物與氧化壓力的影響。運動教練科學，22，47-62。
   連結：
2. 嘉志、姚承義(2007)。以激效作用觀點探討運動之抗老化機轉。運動生理暨體能學報，5，61-70。
   連結：
3. Baillie, J. K.,Bates, M. G.,Thompson, A. A.,Waring, W. S.,Partridge, R. W.,Schnopp, M. F.,Webb, D. J.(2007).Endogenous urate production augments plasma antioxidant capacity in healthy lowland subjects exposed to high altitude.Chest,131(5),1473-1478.
4. Barham, P.,Trinder, P.(1972).An improved colour reagent for the determination of blood glucose by the oxidase system.Analyst,97(151),142-145.
5. Barros, M. P.,Ganini, D.,Lorenco-Lim, L.,Soares, C.O.,Pereira, B.,Bechara, E.J.,Souza-Junior, T.P.(2012).Effects of acute creatine supplementation on iron homeostasis and uric acid-based antioxidant capacity of plasma after wingate test.Journal of the International Society of Sports Nutrition,9(1),25.
6. Cabrera-Crespo, J.,Goncalves, V. M.,Martins, E. A.,Grellet, S.,Lopes, A. P.,Raw, I.(2000).Albumin purification from human placenta.Biotechnology and Applied Biochemistry,31(Pt 2),101-106.
7. Cutrn, J. C.,Perrelli, M. G.,Cavalieri, B.,Peralta, C.,Rosell Catafau, J.,Poli, G.(2002).Microvascular dysfunction induced by reperfusion injury and protective effect of ischemic preconditioning.Free Radical Biology and Medicine,33(9),1200-1208.
8. Davies, G.,Jiang, W. G.,Mason, M. D.(2001).Matrilysin mediates extracellular cleavage of E-cadherin from prostate cancer cells: a key mechanism in hepatocyte growth factor/scatter factor-induced cell-cell dissociation and in vitro invasion.Clinical Cancer Research,7(10),3289-3297.
9. Davies, K. J.,Sevanian, A.,Muakkassah-Kelly, S. F.,Hochstein, P.(1986).Uric acid-iron ion complexes. A new aspect of the antioxidant functions of uric acid.Biochemical Journal,235(3),747-754.
10. Dohm, G.,Beeker, R.T.,Israel, R.G.,Tapscott, E.B.(1986).Metabolic responses to exercise after fasting.Journal of Applied Physiology,61(4),1363-1368.
11. Enomoto, A.,Endou, H.(2005).Roles of organic anion transporters (OATs) and a urate transporter (URAT1) in the pathophysiology of human disease.Clinical and Experimental Nephrology,9(3),195-205.
12. Fossati, P.,Prencipe, L.,Berti, G.(1980).Use of 3,5-dichloro-2-hydroxybenzenesulfonic acid/4-aminophenazone chromogenic system in direct enzymic assay of uric acid in serum and urine.Clinical Chemistry,26(2),227-231.
13. Gasser, G. A.,Brooks, G. A.(1984).Metabolic bases of excess post-exercise oxygen consumption: a review.Medicine and Science in Sports Exercise,16(1),29-43.
14. Giulivi, C.,Davies, K. J.(1990).A novel antioxidant role for hemoglobin. The comproportionation of ferrylhemoglobin with oxyhemoglobin.Journal of Biological Chemistry,265(32),19453-19460.
15. Guindo, A.,Fairhurst, R. M.,Doumbo, O. K.,Wellems, T. E.,Diallo, D. A.(2007).X-linked G6PD deficiency protects hemizygous males but not heterozygous females against severe malaria.Public Library of Science medicine,4(3),e66.
16. Hebbel, R. P.,Eaton, J. W.,Steinberg, M. H.,White, J. G.(1982).Erythrocyte/endothelial interactions in the pathogenesis of sickle-cell disease: a "real logical" assessment.Blood Cells,8(1),163-173.
17. Hellsten, Y.(1994).Xanthine dehydrogenase and purine metabolism in man. With special reference to exercise.Acta Physiologica Scandinavica,621,1-73.
18. Hod, E. A.,Zhang, N.,Sokol, S. A.,Wojczyk, B. S.,Francis, R. O.,Ansaldi, D.,Spitalnik, S. L.(2010).Transftision of red blood cells after prolonged storage produces harmful effects that are mediated by iron and inflammation.Blood,115(21),4284-4292.
19. Hooper, D. C.,Scott, G. S.,Zborek, A.,Mikheeva, T.,Kean, R. B.,Koprowski, H.,Spitsin, S. V.(2000).Uric acid, a peroxynitrite scavenger, inhibits CNS inflammation, blood-CNS barrier permeability changes, and tissue damage in a mouse model of multiple sclerosis.FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology,14(5),691-698.
20. Jean-St-Michel, E.,Manlhiot, C.,Li, J.,Tropak, M.,Michelsen, M. M.,Schmidt, M. R.,Redington, A. N.(2011).Remote preconditioning improves maximal performance in highly trained athletes.Medicine and Science in Sports and Exercise,43(7),1280-1286.
21. Ji, L. L.(1999).Antioxidants and oxidative stress in exercise.Proceedings of the Society for Experimental Biology and Medicine,222(3),283-292.
22. Kaiser, J.(2003).Hormesis. A healthful dab of radiation?.Science,302(5644),378.
23. Kalliovalkama, J.,Jolma, P.,Tolvanen, J. P.,Kahonen, M.,Hutri-Kahonen, N.,Saha, H.,Porsti, I.(1999).Potassium channel-mediated vasorelaxation is impaired in experimental renal failure.American Journal of Physiology,277(4 Pt 2),H1622-1629.
24. Kenney, W. L.,Wilmore, Jack, H.,Costill, David(2011).Physiology of Sport and Exercise.Champaign, IL:Human Kinetics.
25. Kirino, T.(2002).Ischemic tolerance.Journal of Cerebral Blood Flow and Metabolism,22(11),1283-1296.
26. Li, G.,Chen, S.,Lou, W.,Lu, E.(1998).Ischemic preconditioning enhances donor lung preservation in canine lung transplantation.Chinese Medical Journal,111(10),870-873.
27. Lotito, S. B.,Fraga, C. G.(2000).Ascorbate protects (+)-catechin from oxidation both in a pure chemical system and human plasma.Biological Research,33(2),151-157.
28. Mattiazzi, M. D.(2002).Mutated human SOD1 causes dysfunction of oxidative phosphorylation in mitochondria of transgenic mice.The Journal of Biological Chemistry,277(33),29626-29633.
29. McCormick, P. H.,Chen, G.,Tlerney, S.,Kelly, C. J.,Bouchier-Hayes, D. J.(2003).Clinically relevant thermal preconditioning attenuates ischemia- reperfusion injury.The Journal of Surgical Research,109(1),24-30.
30. Meiselman, H. J.,Baskurt, O. K.(2006).Hemorheology and hemodynamics: Dove andare?.Clincal Hemorheol Microcirc,35(1-2),37-43.
31. Murry, C. E.,Jennings, R. B.,Reimer, K. A.(1986).Preconditioning with ischemia: A delay of lethal cell injury in ischemic myocardium.Circulation,74(5),1124-1136.
32. Ng, C. F.,Schafer, F. Q.,Buettner, G. R.,Rodgers, V. G.(2007).The rate of cellular hydrogen peroxide removal shows dependency on GSH: mathematical insight into in vivo H202 and GPx concentrations.Free Radical Research,41(11),1201-1211.
33. Ozdemir, G.,Ozden, M.,Maral, H.,Kuskay, S.,Cetinalp, P.,Tarkun, I.(2005).Malondialdehyde, glutathione, glutathione peroxidase and homocysteine levels in type 2 diabetic patients with and without microalbuminuria.Annals of Clinical Biochemistry,42(Pt 2),99-104.
34. Raeburn, C. D.,Cleveland, J. C.,Zimmerman, M. A.,Harken, A. H.(2001).Organ preconditioning.Archives of Surgery,136(11),1263-1266.
35. Sautin, Y. Y.,Johnson, R. J.(2008).Uric acid: the oxidant-antioxidant paradox.Nucleosides Nucleotides Nucleic Acids,27(6),608-619.
36. Sen, C. K.(1995).Oxidants and antioxidants in exercise.Journal of Applied Physiology,79(3),675-686.
37. Snyder, L. M.,Fortier, N. L.,Trainor, J.,Jacobs, J.,Leb, L.,Lubin, B.,Mohandas, N.(1985).Effect of hydrogen peroxide exposure on normal human erythrocyte deformability, morphology, surface characteristics, and spectrin-hemoglobin cross -linking.Journal of Clinical Investigation,76(5),1971-1977.
38. Veale, M. F.,Healey, G.,Sparrow, R. L.(2011).Effect of additive solutions on red blood cell (RBC) membrane properties of stored RBCs prepared from whole blood held for 24 hours at room temperature.Transfusion,51(Suppl 1),25S-33S.
39. Waring, W. S.,Convery, A.,Mishra, V.,Shenkin, A.,Webb, D. J.,Maxwell, S. R.(2003).Uric acid reduces exercise-induced oxidative stress in healthy adults.Clinical Science (Lond),105(4),425-430.
40. Wheeler, C. R.,Salzman, J. A.,Elsayed, N. M.,Omaye, S. T.,Korte, D. W., Jr.(1990).Automated assays for superoxide dismutase, catalase, glutathione peroxidase, and glutathione reductase activity.Analytical Biochemistry,184(2),193-199.
41. Young, I. S.,Woodside, J. V.(2001).Antioxidants in health and disease.Journal of Clinical Pathology,54(3),176-186.
42. 陸康豪(2009)。碩士論文(碩士論文)。臺北市，國立臺灣師範大學。

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2. 潘怡廷、楊忠祥、林嘉志、林正常、沙部‧魯比(2016)。缺血與運動預處理對力竭運動表現及恢復的影響。大專體育學刊，18(4)，296-309。