短期增補槲皮素對激烈運動後體內抗氧化酵素與丙二醛之影響

Effects of Short-Term Quercetin (QE) Supplementation on Antioxidant Enzymes and Malondialdehyde Induced by Intensive Exercise

10.6127/JEPF.2015.20.03

陳一凡(I-Fan Chen)；吳慧君(Huey-June Wu)；翁明嘉(Ming-Chia Weng)

超氧歧化酶 ； 過氧化氫酶 ； 脂質過氧化物 ； superoxide dismutase ； catalase ； lipid peroxide

運動生理暨體能學報

20輯（2015 / 06 / 01）

23 - 31

繁體中文

目的：探討連續7天增補槲皮素對激烈運動後體內抗氧化酵素與脂質過氧化物的影響。方法：本實驗以隨機雙盲交叉實驗設計，12名健康男大學生參與研究，連續增補7天的槲皮素（Quercetin, QE）2 × 500 mg／天，或安慰劑（Placebo, PL）後，進行單次激烈運動（75%VO_2max）至力竭，在運動前與運動後30及60分鐘，採集血液進行生化值分析。結果：激烈運動後體內抗氧化酵素，包括超氧歧化酶（superoxide dismutase, SOD）與過氧化氫酶（catalase,CAT）活性變化，QE組與PL組間無顯著差異（p ＞ .05）；另外，體內脂質過氧化物丙二醛（malondialdehyde, MDA）的濃度，雖然PL組在運動後30分鐘顯著高於運動前（p ＜ .05），但運動後30分鐘兩組間仍未達顯著差異（p ＞ .05）。結論：短期增補QE對體內抗氧化酵素SOD、CAT與脂質過氧化物MDA並無影響。

Purpose: The aim of this study was to investigate the effect of quercetin (QE) supplementation for seven consecutive days on antioxidant enzymes and lipid peroxide induced by intensive exercise. Method: 12 healthy male collegiate students were recruited and allocated double blindly and randomly into quercetin (QE) supplementation trial (2 × 500 mg/day) or placebo-controlled trial (PL) . The treatment was given for 7 continuous days, and then subjects performed a single bout of intensive exercise (75% VO_2max) until exhaustion. The venous blood samples were drawn before test and at 30 and 60 minutes after completing condition protocol for testing immunological biomarkers. Results: There was no significant difference between QE and PL (p ＞ .05) after intensive exercise in activity of antioxidant enzymes, including superoxide dismutase (SOD) and catalase (CAT). Otherwise, the concentration of lipid peroxide and malondialdehyde in post 30 minutes was higher than pre-test (p ＜ .05) , but yet no significant difference exited between QE and PL (p ＞ .05) at 30 minutes after exercise. Moreover, no significant difference exited in the concentration of lipid peroxide and malondialdehyde. Conclusion: There were no effects of short-term QE intake on antioxidant enzymes, such as SOD, CAT, lipid peroxide and malondialdehyde

1. 陳一凡、吳慧君、張珊綾、翁明嘉(2013)。槲皮素介入對運動表現之影響。大專體育學刊，15(4)，462-471。
   連結：
2. Alavi, Y.,Mirdar, S.,Rostamani, M.(2014).Effects of caffeine ingestions concomitant to incremental running test on lipid peroxidation and enzymatic antioxidant in active men.Iranian Journal of Health and Physical Activity
3. Alessio, H. M.(1993).Exercise-induced oxidative stress.Medicine and Science in Sports and Exercise,25(2),218-224.
4. Arthur, J. R.,Boyne, R.(1985).Superoxide dismutase and glutathione peroxidase activities in neutrophils from selenium deficient and copper deficient cattle.Life sciences,36(16),1569-1575.
5. Borg, G.(1973).Perceived exertion: A note on "history" and methods.Medicine and Science in Sports and Exercise,5(2),90-93.
6. Chang, H. C.,Yang, Y. R.,Wang, P. S.,Wang, R. Y.(2014).Quercetin enhances exercise-mediated neuroprotective effects in brain ischemic rats.Medicine and Science in Sports and Exercis,46(10),1908-1916.
7. Coskun, O.,Kanter, M.,Korkmaz, A.,Oter, S.(2005).Quercetin, a fiavonoid antioxidant, prevents and protects streptozotocin-induced oxidative stress and β-cell damage in rat pancreas.Pharmacological Research,51(2),117-123.
8. Egert, S.,Wolffram, S.,Bosy-Westphal, A.,Boesch-Saadatmandi, C.,Wagner, A. E.,Frank, J.(2008).Daily quercetin supplementation dose-dependently increasesplasma quercetin concentrations in healthy humans.Journal of Nutrition,138(9),1615-1621.
9. Erlund, I.,Silaste, M. L.,Alfthan, G.,Rantala, M.,Kesäniemi, Y. A.,Aro, A.(2002).Plasma concentrations of the flavonoids hesperetin, naringenin and quercetin in human subjects following their habitual diets, and diets high or low in fruit and vegetables.European Journal of Clinical Nutrition,56(9),891-898.
10. Formica, J. V.,Regelson, W.(1995).Review of the biology of quercetin and related bioflavonoids.Food and Chemical Toxicology,33(12),1061-1080.
11. Goulart, M.,Batoreu, M. C.,Rodrigues, A. S.,Laires, A.,Rueff, J.(2005).Lipope roxidation products and thiol antioxidants in chromium exposed workers.Mutagenesis,20(5),311-315.
12. Harwood, M.,Danielewska-Nikiel, B.,Borzelleca, J. F.,Flamm, G. W.,Williams, G. M.,Lines, T. C.(2007).A critical review of the data related to the safety of quercetin and lack of evidence of in vivo toxicity, including lack of genotoxic/carcinogenic properties.Food and Chemical Toxicology,45(11),2179-2205.
13. Ji, L. L.(1999).Antioxidants and oxidative stress in exercise.Experimental Biology and Medicine,222(3),283-292.
14. Johansson, L. H.,Borg, L. A.(1988).A spectrophotometric method for determination of catalase activity in small tissue samples.Analytical biochemistry,174(1),331-336.
15. Kressler, J.,Millard-Stafford, M.,Warren, G. L.(2011).Quercetin and endurance exercise capacity: A systematic review and metaanalysis.Medicine and Science in Sports and Exercise,43(12),2396-2404.
16. Leelayuwat, N.,Laddawan, S.,Kanpetta, Y.,Benja, M.,Wongpan, D.,Tunkamnerdthai, O.(2012).Quercetin enhances endurance capacity via antioxidant activity and size of muscle ribre type 1.Journal of Pharmacy and Nutrition Sciences,2(2),160-164.
17. Noroozi, M.,Burns, J.,Crozier, A.,Kelly, I. E.,Lean, M. E. J.(2000).Prediction of dietary flavonol consumption from fasting plasma concentration or urinary excretion.European Journal of Clinical Nutrition,54(2),143-149.
18. Oyanuagui, Y.(1984).Reevaluation of assay methods and establishment of kit for superoxide dismutase activity.Analytical Biochemistry,142(2),290-296.
19. Powers, S. K.,Jackson, M. J.(2008).Exercise-induced oxidative stress: Cellular mechanisms and impact on muscle force production.Physiological Reviews,88(4),1243-1276.
20. Scholten, S. D.,Sergeev, I. N.(2013).Longterm quercetin supplementation reduces lipid peroxidation but does not improve performance in endurance runners.Open Access Journal of Sports Medicine,4,53.
21. Sen, C. K.,Atalay, M.,Hanninen, O.(1994).Exercise-induced oxidative stress: Glutathione supplementation and de›ciency.Journal of Applied Physiology,77(5),2177-2187.
22. Subudhi, A. W.,Davis, S. L.,Kipp, R. W.,Askew, E.(2001).Antioxidant status and oxidative stress in elite alpine ski racers.International Journal of Sport Nutrition and Exercise Metabolism,11(3),32-41.
23. Tauler, P.,Aguilo, A.,Gimeno, I.,Guix, P.,Tur, J. A.,Pons, A.(2004).Different effects of exercise tests on the antioxidant enzyme activities in lymphocytes and neutrophils.Journal of Nutritional Biochemistry,15(8),479-484.
24. Vider, J.,Lehtmaa, J.,Kullisaar, T.,Vihalemm, T.,Zilmer, K.,Kairane, Č.(2001).Acute immune response in respect to exercise- induced oxidative stress.Pathophysiology,7(4),263-270.
25. 胡國鵬(2011)。博士論文(博士論文)。上海體育學院。
26. 董改寧、熊正英(2005)。槲皮素對運動訓練大鼠肝組織自由基代謝及運動能力影響實驗研究。陝西師範大學學報（自然科學版），33(1)，102-104。
27. 蔡佈曦、甘能斌、劉介仲、曾明郎、何素珍、林志成(2004)。單次衰竭運動對脂質過氧化物指標的影響。大專體育英文學刊，4，77-86。