抗氧化性虱目魚皮明膠水解物製程與特性探討

Production and Characterization of Antioxidant Gelatin Hydrolysate from Milkfish Skin

10.6983/KHHYKTHP.201003.0027

楊景雍(Jing-Iong Yang)；龔得安(Te-An Kung)；楊博仲(Bo-Zhong Yang)

抗氧化性 ； 虱目魚 ； 明膠水解物 ； 熱水解 ； 超過濾 ； antioxidant activity ； milkfish ； gelatin hydrolysate ； thermal hydrolysis ； ultrafiltration

高雄海洋科大學報

24期（2010 / 03 / 01）

27 - 42

繁體中文

The present study demonstrates that milkfish retorted skin gelatin hydrolysates (RSGH) possess antioxidant activities including DPPH scavenging, reducing power, lipid peroxidation inhibition, and protective effect of DNA oxidative damage. The antioxidant activities of RSGH derived from different levels of thermal hydrolysis (from 0.5 to 3 h) showed skin gelatin retorted for 3 h (RSGH-3h) had the highest antioxidant activity. Besides, the main molecular weight ranges of the RSGH-0.5h RSGH-1h, RSGH-2h, and RSGH-3h were＞6511 (62.48%), 686~12500 (87.49), 189~6511 (81.39%), and 189~6511 Da (84.44%), respectively. After sequential ultrafiltration with various MWCO ranges, 1k-RSGH-3h ultrafiltrated from RSGH-3h was the most strong antioxidant activity among 1k-RSGH-3h (MWCO＜1kDa), 5-1k-RSGH-3h (MWCO: 1~5 kDa), 10-5k-RSGH-3h (MWCO: 5~10 kDa), and 10k-RSGH-3h (MWCO＞10 kDa). It indicated that oligopeptides in RSGHs play an important role in their antioxidant activities. Moreover, milkfish 1k-RSGH-3h shows great potential as a natural antioxidant.

The present study demonstrates that milkfish retorted skin gelatin hydrolysates (RSGH) possess antioxidant activities including DPPH scavenging, reducing power, lipid peroxidation inhibition, and protective effect of DNA oxidative damage. The antioxidant activities of RSGH derived from different levels of thermal hydrolysis (from 0.5 to 3 h) showed skin gelatin retorted for 3 h (RSGH-3h) had the highest antioxidant activity. Besides, the main molecular weight ranges of the RSGH-0.5h RSGH-1h, RSGH-2h, and RSGH-3h were＞6511 (62.48%), 686~12500 (87.49), 189~6511 (81.39%), and 189~6511 Da (84.44%), respectively. After sequential ultrafiltration with various MWCO ranges, 1k-RSGH-3h ultrafiltrated from RSGH-3h was the most strong antioxidant activity among 1k-RSGH-3h (MWCO＜1kDa), 5-1k-RSGH-3h (MWCO: 1~5 kDa), 10-5k-RSGH-3h (MWCO: 5~10 kDa), and 10k-RSGH-3h (MWCO＞10 kDa). It indicated that oligopeptides in RSGHs play an important role in their antioxidant activities. Moreover, milkfish 1k-RSGH-3h shows great potential as a natural antioxidant.