Bioinformatics Analysis of the Duck Enteritis Virus UL54 Gene

Chao-Yue Liu；An-Chun Cheng；Ming-Shu Wang

DEV ； molecular characterization ； sequence analysis ； UL54

Research Journal of Applied Sciences, Engineering and Technology

7卷14期（2014 / 04 / 12）

2813 - 2817

英文

In this study, we analyze the Duck Enteritis Virus (DEV) UL54 gene, which has been isolated and identified in our lab (GenBank accession NO EU071033), to help deeply research on DEV. DNA sequence analysis showed that the identified ORF which composed of 1377 bp nucleotides encoded 458 amino acids with a predicted Mr. of 51.75 kDa. Multiple sequence alignment suggested that the UL54 gene was highly conserved in Alphaherpesvirinae and was similar to the other herpesviral UL54 gene. Phylogenetic analysis of the DEV UL54 gene revealed that DEV had a close evolutionary relationship with Gallid, Herpesvirus 2 (GaHV-2), Gallid Herpesvirus 3 (GaHV-3), Meleagrid Herpesvirus1 (MeHV-1) and should belong to a single cluster within the Alphaherpesvirinae subfamily.