Molecular Detection and Viral Isolation of Newcastle Disease Viruses from Chickens in Benue State, Nigeria

10.19026/ijava.8.3340

H.O. Abah；P.A. Abdu；A. Assam

Chicken ； live bird market ； Newcastle disease virus ； Nigeria ； RT-PCR ； village households

International Journal of Animal and Veterinary Advances

8卷4期（2016 / 11 / 20）

40 - 45

英文

The present research work was carried out to detect and isolate Newcastle Disease Virus (NDV) from apparently healthy chickens and evaluate their role in Newcastle disease outbreak.A survey on the prevalence of Newcastle Disease Virus (NDV) was conducted in six Local Government Areas (LGAs) of Benue State, Nigeria. A total of 250 cloacal and tracheal swabs were collected from apparently healthy chickens from commercial farms, Live Bird Markets (LBMs) and village households. Reverse Transcription-Polymerase Chain Reaction (RT-PCR) was used to detect Newcastle Disease Viruses (NDV) while viral isolation was performed using embryonated chicken eggs. The overall detection rate for ND Viruses (NDVs) was 12% (30/250). Newcastle disease viruses were detected in all sampling units with the highest viral detection of 16.1% (9/56) from chickens in LBMs. The overall NDV isolation rate was 20% (6/30), isolates were from Kwande and Makurdi LGAs with village chickens from LBMs (55.6%) recording the highest isolation rate. Chickens from LBMs, Kwande and Makurdi LGAs may serve as sources of NDVs to those in commercial farms and village households. The vaccination of village chickens should be considered as important in the control of ND in Benue State, Nigeria.