小腦神經免疫組織化學染色在不同死因的表現

The Immunohistochemical Studies in Cerebellum on Various Causes of Death

10.6134/tjfm.2010.0201.15

施懿晉(Yi-Jin Shih)；林偉隆(Wea-Lung Lin)；蔡崇弘(Chung-Hung Tsai)；陳茹紅(Ju-Hung Chen)；趙婉如(Wan-Ru Chao)

小腦 ； 普金吉氏細胞 ； 死因 ； GFAP ； S-100 ； NSE ； Cerebellum ； Purkinje cell ； Death cause ； GFAP ； S-100 ； NSE

台灣法醫學誌

2卷1期（2010 / 06 / 01）

35 - 41

繁體中文

小腦是人類的重要器官之一。腦部神經元受損的生物學變化已經被廣泛的研究，但是只有少數的研究是有關人類小腦組織學的變化與死因的相關性。此篇研究收錄了2006年1月至7月彰化和南投地檢署的43個法醫解剖個案，死因包括心臟、肺部或腦部的功能喪失。用蘇木素―嗜伊紅性染色研究普金吉氏細胞的型態學、數量及比較三種免疫染色標記（GFAP、S-100及NSE）在不同死因的表現。普金吉氏細胞的數量、大小及細胞質的呈色在不同的死因並沒有明顯差異。普金吉氏細胞不論在何種死因都大量表現NSE，但在心因性死亡的個案中，NSE在小腦的顆粒層及分子層的染色較強，表示與腦部或肺部相關的死因個案相比，其對腦部的影響較小。

The cerebellum is a critical human organ. The biological change in damaged neurons of the brain had been extensively researched, but few studies have examined histological and biological expression cerebellar variants in relation to death. Presently, 43 forensic autopsies were performed from the Prosecutor's Office of Chang-Hua and Nantou in Taiwan between January and July, 2006. Causes of death were due to malfunction of the brain (n=7), heart (n=22), and lung (n=14). Hematoxylin-Eosin staining was used to study Purkinje cell morphology and quantity, and the expression of three biomarkers (GFAP, S-100 and NSE) among all the causes of death. There was no difference in Purkinje cell size, number, and cytoplasm color. Immunohistochemical staining revealed GFAP expression in two cases of lung-related death, despitethe rarity of the biomarker in Purkinje cell. NSE immune reaction was strong but unstable in the cerebellar cortex granular and molecular layers from heart-related deaths indicating less brain damage in heart related death than in brain or lung related death.

1. Karhune, PJ,Erkinjuntti, T,Laippala, P(1994).Moderate alcohol consumption and loss of cerebellar purkinje cells.BMJ,308,1663-7.
2. Kuroiwa, T,Tanabe, H,Takatsuka, H,Arai, M,Nagasawa, S,Ohta, T(1993).Significance of serum neuron-specific enolase levels after head injury.No Shinkei Geka,21,1021-4.
3. Lavezzi, AM,Ottaviani, G,Terni, L,Matturri, L(2006).Histological and biological developmental characterization of the human cerebellar cortex.Int J Dev Neurosci,24,365-71.
4. Li, DR,Zhu, BL,Ishikawa, T,Zhao, D,Michiue, T,Maeda, H(2006).Immunohistochemical distribution of S-100 protein in the cerebral cortex with regard to the cause of death in forensic autopsy.Leg Med,8,78-85.
5. Nogami, M,Takatsu, A,Ishiyama, I(1998).Immunohistochemical study of neuron-specific enolase in human brains from forensic autopsies.Forensic Sci Int,94,97-109.
6. Ross, SA,Cunningham, RT,Johnston, CF,Rowlands, BJ(1996).Neuron-specific enolase as an aid to outcome prediction in head injury.Br. J. Neurosurg,10,471-6.
7. Schmechel, D,Marangos, PJ,Brightman, M(1978).Neurone-specific enolase is a molecular marker for peripheral and central neuroendocrine cells.Nature,276,834-6.
8. Schmechel, D,Marangos, PJ,Zis, AP,Brightman, M,Goodwin, FK(1978).Brain enolases as specific markers of neuronal and glial cells.Science,199,313-5.
9. Sriram, K,Benkovic, SA,Miller, DB,O'Callaghan, JP(2002).Obesity exacerbates chemically induced neurodegeneration.Neuroscience,115,1335-46.
10. Uzan, M,Hanci, M,Güzel, Ö(1995).The significance of neuron specific enolase levels in cerebrospinal fluid and serum after experimental traumatic brain damage.Acta. Neurochir,135,141-3.
11. Watanabe, M,Sakimura, K,Takahashi, Y,Kondo, H(1990).Ontogenic changes in expression of neuron-specific enolase (NSE) and its mRNA in the purkinje cells of the rat cerebellum: Immunohistochemical and in situ hybridization study.Brain Res. Dev. Brain Res,53,89-9.