题名

體液鑑定方法之探討

并列篇名

Overviews of the Methods for Body Fluid Identification

DOI

10.6134/tjfm.2014.0601.01

作者

楊雅玲(Ya-Ling Yang);謝幸媚(Hsing-Mei Hsieh);李俊億(James Chun-I Lee);洪振翔(Chen-Hsiang Hung);張宇翔(Yu-Hsiang Chang);蔡麗琴(Li-Chin Tsai)

关键词

體液 ; 體液鑑定 ; 刑事鑑識 ; Body Fluid ; Body Fluid Identification ; Forensic Science

期刊名称

台灣法醫學誌

卷期/出版年月

6卷1期(2014 / 06 / 01)

页次

1 - 16

内容语文

繁體中文

中文摘要

體液檢體是犯罪現場中常見的生物跡證。刑案調查中除了追尋生物跡證的個化來源,該證物屬於何種體液或組織亦是越來越迫切需要提供的資訊。體液種類或其來源的資訊可於犯罪偵查階段釐清案情以指引偵查方向,幫助鑑識人員重建犯罪現場之發生事實或犯罪行為,更可提供法官作為審判依據。生物檢體中特定細胞、蛋白或RNA的表現程度若具有組織或細胞專一性亦可用於檢測體液檢體的來源或種類,目前已有多種技術方法應用於體液鑑別上,包括顯微鏡檢法、結晶試驗、呈色試驗或催化試驗、層析法、電泳法、光譜法、免疫分析法以及生物醫學界中常見的分生技術等。體液鑑別對於生物鑑識領域漸趨重要,本文針對目前鑑識領域之相關研究或實務上應用於體液鑑定之方法及近來具發展潛力的技術方法進行探討。

英文摘要

Body fluids are the common biological evidences in crime scenes. In addition to individualize the biological evidences, the information about the body fluid types or tissue origins is requested more and more. Information of body fluid origins not only helps the investigators to clarify the details of cases but also assists the forensic officers to reconstruct the criminal activities in the crime scene and further as the judgment basis for the judiciary. Cell types and protein or RNA expressions with tissue or cell-specificity of biological samples can be used as biomarkers to identify the origins or types of body fluids. Nowadays numerous techniques have been developed to identify the body fluids, including alternative light source methods, microscopic examination, crystal testing, color tests, catalytic tests, chromatographic methods, electrophoresis, spectroscopic methods, immunoassays, biomedical technology, etc. As the increasing importance of body fluid identification, this report provides an overview of the identification methods which have been applied on forensic science and the approaches still under development lately.

主题分类 醫藥衛生 > 基礎醫學
社會科學 > 法律學
参考文献
  1. 謝幸媚、盧皇德、王俊翔(2013)。精液鑑別方法及DNA鑑定系統靈敏度之評估。刑事科學,75,49-62。
    連結:
  2. Powers LS, Lloyd CR: Method and apparatus for detecting and imaging the presence of biological materials. US 7186990 B2 United States: Micro Biosystems, Limited Partnership, Cheyenne, WY, 2004..
  3. Akutsu, T,Motani, H,Watanabe, K,Iwase, H,Sakurada, K(2012).Detection of bacterial 16S ribosomal RNA genes for forensic identification of vaginal fluid.Leg Med,14,160-2.
  4. Akutsu, T,Watanabe, K,Sakurada, K(2012).Specificity, sensitivity, and operability of rsid™-urine for forensic identification of urine: Comparison with ELISA for tamm-horsfall protein.J Forensic Sci,57,1570-3.
  5. Allery, JP,Telmon, N,Mieusset, R,Blanc, A,Rougé, D(2001).Cytological detection of spermatozoa: Comparison of three staining methods.J Forensic Sci,46,349-51.
  6. Alvarez, M,Ballantyne, J(2006).The identification of newborns using messenger RNA profiling analysis.Anal Biochem,357,21-34.
  7. An, J,Choi, A,Shin, KJ,Yang, WI,Lee, HY(2013).DNA methylation-specific multiplex assays for body fluid identification.Int J Legal Med,127,35-43.
  8. An, JH,Shin, KJ,Yang, WI,Lee, HY(2012).Body fluid identification in forensics.BMB Rep,45,545-53.
  9. Andersen, J,Bramble, S(1997).The effects of fingermark enhancement light sources on subsequent PCRSTR DNA analysis of fresh bloodstains.J Forensic Sci,42,303-6.
  10. Bartel, DP(2004).MicroRNAs: Genomics, biogenesis, mechanism, and function.Cell,116,281-97.
  11. Bauer, M,Patzelt, D(2003).A method for simultaneous RNA and DNA isolation from dried blood and semen stains.Forensic Sci Int,136,76-8.
  12. Bauer, M,Patzelt, D(2002).Evaluation of mRNA markers for the identification of menstrual blood.J Forensic Sci,47,1278-82.
  13. Bauer, M,Patzelt, D(2008).Identification of menstrual blood by real time RT-PCR: Technical improvements and the practical value of negative test results.Forensic Sci Int,174,55-9.
  14. Bauer, M,Polzin, S,Patzelt, D(2003).Quantification of RNA degradation by semi-quantitative duplex and competitive RT-PCR: A possible indicator of the age of bloodstains?.Forensic Sci Int,138,94-103.
  15. Bowden, A,Fleming, R,Harbison, S(2011).A method for DNA and RNA co-extraction for use on forensic samples using the Promega DNA IQ™ system.Forensic Sci Int Genet,5,64-8.
  16. Bremmer, RH,Edelman, G,Vegter, TD,Bijvoets, T,Aalders, MC(2011).Remote spectroscopic identification of bloodstains.J Forensic Sci,56,1471-5.
  17. Byun, H-M,Siegmund, KD,Pan, F(2009).Epigenetic profiling of somatic tissues from human autopsy specimens identifies tissue- and individual-specific DNA methylation patterns.Hum Mol Gene,18,4808-17.
  18. Casey, DG,Price, J(2010).The sensitivity and specificity of the RSID™-saliva kit for the detection of human salivary amylase in the Forensic Science Laboratory, Dublin, Ireland.Forensic Sci Int,194,67-71.
  19. Edelman, G,van, Leeuwen, TG,Aalders, MCG(2012).Hyperspectral imaging for the age estimation of blood stains at the crime scene.Forensic Sci Int,223,72-7.
  20. Edelman, GJ,Gaston, E,van Leeuwen, TG,Cullen, PJ,Aalders, MCG(2012).Hyperspectral imaging for noncontact analysis of forensic traces.Forensic Sci Int,223,28-39.
  21. Edelman, GJ,van, Leeuwen, TG,Aalders, MCG(2013).Hyperspectral imaging of the crime scene for detection and identification of blood stains.Proceedings of SPIE
  22. Estes, C,Duncan, A,Wade, B,Lloyd, C,Ellis, W, Jr,Powers, L(2003).Reagentless detection of microorganisms by intrinsic fluorescence.Biosens Bioelectron,18,511-9.
  23. Fleming, RI,Harbison, S(2010).The use of bacteria for the identification of vaginal secretions.Forensic Sci Int Genet,4,311-5.
  24. Frascione, N,Thorogate, R,Daniel, B,Jickells, S(2012).Detection and identification of body fluid stains using antibody-nanoparticle conjugates.Analyst,137,508-12.
  25. Frumkin, D,Wasserstrom, A,Budowle, B,Davidson, A(2011).DNA methylation-based forensic tissue identification.Forensic Sci Int Genet,5,517-24.
  26. Gaensslen, RE(1983).Sourcebook in Forensic Serology, Immunology, and Biochemistry.Washington, DC:U.S. Dept. of Justice, National Institute of Justice USA, National Institute of Justice.
  27. Giampaoli, S,Berti, A,Valeriani, F(2012).Molecular identification of vaginal fluid by microbial signature.Forensic Sci Int Genet,6,559-64.
  28. Haas, C,Klesser, B,Bär, W,Kratzer, A(2008).mRNA profiling for body fluid identification by reverse transcription endpoint PCR and realtime PCR.Forensic Sci Int Genet Supplement Series,1,37-8.
  29. Hanson, EK,Ballantyne, J(2013).Highly specific mRNA biomarkers for the identification of vaginal secretions in sexual assault investigations.Sci Justice,53,14-22.
  30. Hanson, EK,Lubenow, H,Ballantyne, J(2009).Identification of forensically relevant body fluids using a panel of differentially expressed microRNAs.Anal Biochem,387,303-14.
  31. Hedman, J,Dalin, E,Rasmusson, B,Ansell, R(2011).Evaluation of amylase testing as a tool for saliva screening of crime scene trace swabs.Forensic Sci Int Genet,5,194-8.
  32. Hochmeister, MN,Budowle, B,Rudin, O(1999).Evaluation of prostate-specific antigen (PSA) membrane test assays for the forensic identification of seminal fluid.J Forensic Sci,44,1057-60.
  33. James, SH(ed.),Nordby, JJ(ed.)(2005).Forensic Science: An Introduction to Scientific and Investigative Techniques.Boca Raton, FL:CRC Press.
  34. Juusola, J,Ballantyne, J(2007).mRNA profiling for body fluid identification by multiplex quantitative RTPCR.J Forensic Sci,52,1252-62.
  35. Juusola, J,Ballantyne, J(2005).Multiplex mRNA profiling for the identification of body fluids.Forensic Sci Int,152,1-12.
  36. Lee, HC(ed.),Gaensslen, RE(ed.)(1986).Advances in Forensic Science.Seal Beach, CA:Biomedical Publications.
  37. Lee, HC(ed.),Gaensslen, RE(ed.)(1985).Advances in Forensic Science.Foster City, CA:Biomedical Publications.
  38. Lee, HY,Park, MJ,Choi, A,An, JH,Yang, WI,Shin, KJ(2012).Potential forensic application of DNA methylation profiling to body fluid identification.Int J Legal Med,126,55-62.
  39. Lee, W-C,Khoo, B-E(2010).Forensic light sources for detection of biological evidences in crime scene investigation: A review.Malaysian J Forensic Sci,1,17-27.
  40. Lin, AC,Hsieh, HM,Tsai, LC,Linacre, A,Lee, JC(2007).Forensic applications of infrared imaging for the detection and recording of latent evidence.J Forensic Sci,52,1148-50.
  41. Lindenbergh, A,de Pagter, M,Ramdayal, G(2012).A multiplex (m)RNA-profiling system for the forensic identification of body fluids and contact traces.Forensic Sci Int Genet,6,565-77.
  42. Liu, B,Lague, JR,Nunes, DP(2002).Expression of membrane-associated mucins MUC1 and MUC4 in major human salivary glands.J Histochem Cytochem,50,811-20.
  43. Madi, T,Balamurugan, K,Bombardi, R,Duncan, G,McCord, B(2012).The determination of tissue-specific DNA methylation patterns in forensic biofluids using bisulfite modification and pyrosequencing.Eletrophoresis,33,1736-45.
  44. Misencik, A,Laux, DL(2007).Validation study of the seratec hemdirect hemoglobin assay for the forensic identification of human blood.Midwestern Association of Forensic Science, Newsletter,Spring,18-26.
  45. Mitch, J(2008).Clues at the scene of a crime: Advances in forensics push the limits of trace analysis and raise public interest in science.Chemical & Engineering News,86,59-60.
  46. Nakanishi, H,Kido, A,Ohmori, T(2009).A novel method for the identification of saliva by detecting oral streptococci using PCR.Forensic Sci Int,183,20-3.
  47. Nussbaumer, C,Gharehbaghi-Schnell, E,Korschineck, I(2006).Messenger RNA profiling: A novel method for body fluid identification by Real-Time PCR.Forensic Sci Int,157,181-6.
  48. Ong, SY,Wain, A,Groombridge, L,Grimes, E(2012).Forensic identification of urine using the DMAC test: A method validation study.Sci Justice,52,90-5.
  49. Pang, BC,Cheung, BK(2007).Identification of human semenogelin in membrane strip test as an alternative method for the detection of semen.Forensic Sci Int,169,27-31.
  50. Rando, OJ,Verstrepen, KJ(2007).Timescales of genetic and epigenetic inheritance.Cell,128,655-68.
  51. Saferstein, R(ed.)(2002).Forensic Science Handbook.Upper Saddle River, NJ:Prentice Hall.
  52. Saferstein, R(ed.)(2002).Forensic Science Handbook.Upper Saddle River, NJ:Prentice Hall.
  53. Sakurada, K,Akutsu, T,Fukushima, H,Watanabe, K,Yoshino, M(2010).Detection of dermcidin for sweat identification by real-time RT-PCR and ELISA.Forensic Sci Int,194,80-4.
  54. Schweers, BA,Old, J,Boonlayangoor, PW,Reich, KA(2008).Developmental validation of a novel lateral flow strip test for rapid identification of human blood (Rapid Stain Identification™ -Blood).Forensic Sci Int Genetics,2,243-7.
  55. Sikirzhytskaya, A,Sikirzhytski, V,Lednev, IK(2012).Raman spectroscopic signature of vaginal fluid and its potential application in forensic body fluid identification.Forensic Sci Int,216,44-8.
  56. Sikirzhytski, V,Sikirzhytskaya, A,Lednev, IK(2012).Advanced statistical analysis of Raman spectroscopic data for the identification of body fluid traces: Semen and blood mixtures.Forensic Sci Int,222,259-65.
  57. Sood, P,Krek, A,Zavolan, M,Macino, G,Rajewsky, N(2006).Cell-type-specific signatures of microRNAs on target mRNA expression.Proc Natl Acad Sci US A,103,2746-51.
  58. Toates, P(1979).The forensic identification of semen by isoelectric focusing of seminal acid phosphatase.Forensic Sci Int,14,191-214.
  59. Trombka, JI,Schweitzer, J,Selavka, C(2002).Crime scene investigations using portable, nondestructive space exploration technology.Forensic Sci Int,129,1-9.
  60. Vandenberg, N,van Oorschot, RA(2003).The use of Polilight® in the detection of seminal fluid, saliva, and bloodstains and comparison with conventional chemical-based screening tests.J Forensic Sci,51,361-70.
  61. Vidaki, A,Daniel, B,Court, DS(2013).Forensic DNA methylation profiling - Potential opportunities and challenges.Forensic Sci Int Genet,7,499-507.
  62. Virkler, K,Lednev, IK(2009).Analysis of body fluids for forensic purposes: From laboratory testing to nondestructive rapid confirmatory identification at a crime scene.Forensic Sci Int,188,1-17.
  63. Virkler, K,Lednev, IK(2008).Raman spectroscopy offers great potential for the nondestructive confirmatory identification of body fluids.Forensic Sci Int,181,e1-e5.
  64. Wasserstrom, A,Frumkin, D,Davidson, A,Shpitzen, M,Herman, Y,Gafny, R(2013).Demonstration of DSIsemen - A novel DNA methylation-based forensic semen identification assay.Forensic Sci Int Genet,7,136-142.
  65. Yang, H,Zhou, B,Deng, H,Prinz, M,Siegel, D(2013).Body fluid identification by mass spectrometry.Int J Legal Med,127,1065-77.
  66. Yu, H,Diamandis, EP(1995).The milk of lactating women contains prostate specific antigen.Clinical Biochemistry,28,341.
  67. Zubakov, D,Boersma, AWM,Choi, Y,van Kuijk, PF,Wiemer, EAC,Kayser, M(2010).MicroRNA markers for forensic body fluid identification obtained from microarray screening and quantitative RT-PCR confirmation.Int J Legal Med,124,217-26.
  68. 章雅清、陸惠玲、姚亞楠(2012)。增強化學發光法檢測基質金屬蛋白酶-11鑒定月經血。增強化學發光法檢測基質金屬蛋白酶-11鑒定月經血。法醫學雜誌 2012;2:109-11.,2,109-11。
  69. 詹世鵬(2010)。小兵立大功―微小核醣核酸與小干擾核醣核酸。化學,68,303-12。
  70. 蔡東霖、陳用佛(2013)。高光譜影像技術在鑑識科學上之應用。警學叢刊,43,1-15。