液相層析法分析茶飲料中兒茶素

Determination of Catechins in Tea Drinks by High Performance Liquid Chromatography

10.6945/ARFDR.201112.0092

吳白玟(Pei-Wen Wu)；劉曉芸(Hsiao-Yun Liu)；鄭郁琪(Yu-Chi Cheng)；曾素香(Su-Hsiang Tseng)；蘇淑珠(Shu-Chu Su)；闕麗卿(Lih-Ching Chiueh)

茶飲料 ； 兒茶素 ； 咖啡因 ； 高效液相層析 ； tea drink ； catechin ； caffeine ； HPLC

食品藥物研究年報

2期（2011 / 12 / 08）

90 - 96

繁體中文

本研究係建立利用高效液相層析儀（high performance liquid chromatograph，HPLC）配合光二極體陣列偵測器（photodiode array detector，PDA），檢測茶飲料中8種兒茶素之檢驗方法。檢體經稀釋、濾膜過濾後即可以Agilent Eclipse Plus C18（5μm，內徑4.6mm×25cm）液相層析管柱，配合添加0.1%甲酸之去離子水及乙腈溶液作為移動相梯度之條件下，依流速1mL／min以高效液相層析儀配合光二極體陣列偵測器於波長280nm進行檢測，可將8種兒茶素與咖啡因（caffeine）分離。進行22件茶飲料健康食品之兒茶素標示符合性之評估，其兒茶素檢測含量範圍介於24－96mg/mL之間，除年初抽驗之4件茶飲料檢體外，其餘陸續抽驗之18件檢體，其兒茶素檢出量均與標示相符，顯示茶飲料兒茶素標示不符之情形確有改善。

A method was developed for the determination catechins in tea drinks by high performance liquid chromatography (HPLC) with photodiode array detector (PAD). Samples were diluted with 0.1% phosphoric acid solution appropriately, eluted in stepwise with 0.1% formic acid (prepared with deionized water) and acetonitrile at flow rate 1 mL/min through an Agilent Eclipse Plus C18 column (4.6 mm i.d. × 25 cm, 5 μm) and detected at 280 nm wavelength. Eight kinds of catechins and caffeine in tea drinks were well separated. The amounts of catechins detected from 22 samples were in the range of 24-96 mg/mL. Except for 4 samples, the amounts of catechins detected in the rest 18 samples were all coincided with the labeling regulation.