嬰兒奶嘴中矽氧烷之檢驗方法之探討

Investigation and Establishment of the Test Method for Siloxanes in Silicone Nipples

張美華(Mei-Hua Chang)；丁悅(Yueh Ting)；鄒嘉珊(Chia-Shan Tsou)；曾素香(Su-Hsiang Tseng)；高雅敏(Ya-Min Kao)；周秀冠(Hsiu-Kuan Chou)；陳惠芳(Hwei-Fang Cheng)

矽氧烷 ； 矽膠奶嘴 ； 氣相層析串聯質譜儀 ； siloxane ； silicon nipple ； gas chromatograph/tandem mass spectrometer

食品藥物研究年報

6期（2015 / 12 / 01）

10 - 21

繁體中文

本研究以矽膠材質之嬰兒奶嘴為研究對象，探討檢測低分子量之直鏈矽氧烷及環狀矽氧烷之含量及其溶出情形之檢驗方法。奶嘴檢體經超低溫研磨機均質，取0.2g以正己烷10 mL萃取；另以正庚烷進行溶出試驗，檢液均以氣相層析串聯質譜儀(GC/MS/MS)配合多重反應偵測(MRM)模式分析。檢驗方法之確效部分，於檢體中添加0.2、0.5及2.5 μg/g之直鏈矽氧烷及5、12.5 μg/g之環狀矽氧烷，5重複之平均回收率介於77-120%，變異係數皆小於9%。以建立之檢驗方法進行市售9件不同品牌矽膠奶嘴中矽氧烷之檢測，結果環狀矽氧烷含量範圍為1.8-53.1 μg/g，直鏈矽氧烷含量範圍為未檢出-0.56 μg/g；溶出試驗結果6件檢出直鏈矽氧烷，溶出範圍為0.017-0.098 μg/mL，9件檢體均溶出環狀矽氧烷，溶出範圍為0.028-1.325 μg/mL，本研究結果將提供行政管理單位參考。

A method was developed to determine the content and migration of low-molecular-weight (LMW) siloxanes including linear and cyclic forms in baby silicone nipples. For content testing, each nipple samples was cut into small pieces, homogenized using cryogenic grinding with liquid nitrogen, and then the ground nipple powder (0.2 g) was extracted with 10 mL of n-hexane. For migration testing of content, each nipple samples was rinsed with n-heptane, and the solvent was collected as sample solution. LMW siloxane detection was done utilizing gas chromatograph/tandem mass spectrometer (GCiMSIMS) operated at multiple reaction monitoring (MRM) mode. Blank samples were fortified willi different levels of siloxanes including 0.2, 0.5, 2.5, 5 and 12.5 μg/g for recovery studies. The recoveries of siloxanes were from 77 to 120% with coefficients of variation (CVs) less than 9% (n=5). The established methods were applied for survey of 9 different brands of commercially baby silicone nipples. The results showed the contents of cyclic and linear siloxanes were found to be from 1.8 to 53.1 μg/g and from not detected to 0.56 μg/g, respectively. In the migration test, 6 and 9 samples were detected of cyclic siloxanes from 0.017 to 0.098 μg/ml. and linear siloxanes from 0.028 to 1.325 μg/ml., respectively. The reports of this studies will provide to authorities for references.