利用離子層析法分析牙膏及漱口水中游離氟化物(F^-)及單氟磷酸鹽(PO_3F^(2-))

Simultaneous Determination of Fluoride and Monofluorophosphate in Toothpastes and Mouth Rinses by Ion Chromatography

陳信豪(Shin-Hao Chen)；黃守潔(Shou-Chieh Huang)；陳玉盆(Yu-Pen Chen)；周秀冠(Hsiu-Kuan Chou)；陳惠芳(Hwei-Fang Cheng)

游離氟 ； 單氟磷酸鹽 ； 離子層析法 ； free fluoride ； monofluorophosphate ； ion chromatography

食品藥物研究年報

6期（2015 / 12 / 01）

264 - 269

繁體中文

本研究係利用離子層析法(ion chromatography)，建立同時檢測牙膏(toothpaste)及漱口水(mouthwash)中游離氟化物(F^-)及單氟磷酸鹽(PO_3F^(2-))成分之檢測方法。檢體以去離子水溶解後，經IonPacRAS11-HC離子層析管柱(內徑4 × 250 mm)；採用100 mM氫氧化鈉溶液及去離子水為移動相進行梯度流洗；流速為1.0 mL/min，再以電導度偵測器進行分析。2種成分標準曲線線性範圍為2.26-45.25 μg/mL，判定係數R^2皆為0.998以上，於牙膏中添加游離氟及單氟磷酸鹽不同濃度之標準品溶液，回收率介於87.9-109.3%，相對標準偏差均小於0.96%。最低定量極限分別為游離氟45.2 ppm及單氟磷酸鹽68.0 ppm。應用本研究所建立之方法檢測市售牙膏18件及漱口水11件，結果18件牙膏中，17件檢出總氟化物含量介於336.8-1453.6 ppm，1件未檢出；漱口水11件，檢出總氟化物含量介於67.1-284.4 ppm，本方法將提供各界檢驗牙膏及漱口水中游離氟化物及單氟磷酸鹽成分之參考。

In this study, ion chromatography (IC) was applied for the simultaneous determination of fluoride and monofluorophosphate in toothpastes and mouth rinses. The samples were dissolved in deionized water and then analyzed by an IC system consisted of an IonPacRAS11-HC (I D 4 × 250 mm) column and a gradient pump. The gradient elution consisted of 100 mM sodium hydroxide solution and deionized water was programmed at a flow rate of 1.0 mL/min. The linear range of standards was 2.26-45.25 μg/mL with R^2 all above 0.998. The recoveries of fluoride and monofluorophosphate ranged from 87.9 to 109.3% with coefficients of variation, all less than 0.96% were conducted by spiking levels of standards into toothpaste. The limit of quantification were 45.2 ppm and 68.0 ppm for free fluoride and monofluorophosphate, respectively. The established method was applied for survey of 18 and 11 commercial toothpastes and mouth rinses purchased from retail stores, respectively. The results showed that the free fluorine was found in 17 out of 18 toothpastes ranged from 336.8 to 1453.6 ppm, and in 11 mouth rinses ranged from 67.1 to 284.4 ppm. The method will provide as a reference method for the detection of fluoride and monofluorophosphate in toothpastes and mouth rinses.