南臺灣族群MxA基因起動子之核苷酸序列分析之研究

Analysis of MxA gene Promoter from Southern Min Group of Southern Taiwanese

10.7060/KNUJST.201112.0003

王惠亮(Hui-Liang Wang)；巴子晨(Tz-Cheng Ba)

MxA基因 ； 起動子 ； 單核苷酸多型性 ； 類似干擾素刺激反應序列 ； 干擾素 ； MxA gene ； Promoter ； single nucleotide polymorphism (SNP) ； interferon stimulated response element like sequence (ISRE-like sequence) ； Interferon

高雄師大學報：自然科學與科技類

31期（2011 / 12 / 01）

3 - 17

英文

The human MxA protein is an interferon-inducible protein that confers resistance to influenza virus and other RNA viruses. MxA proteins are synthesized in response to stimulation by type I (α⁄β) interferon in specific organ. Gene expression suggested that at least a region of nucleotide sequence in the MxA gene promoter contains ISRE (interferon stimulated response element). The nucleotide sequences of MxA gene promoters of 30 randomly selected individuals of Southern Min group in southern Taiwan were analyzed. There was no apparent TATA box or CAAT box but a GC-rich region in the MxA gene promoter. Identity of nucleotide sequence of MxA gene promoter from Southern Min group of southern Taiwanese was 98% in comparison with that of NCBI (National Center for Biotechnology Information) GenBank data library. The result demonstrated that a Guanylic acid and a Cytidylic acid were inserted between nucleotide positions -2 and -3 as well as -32 and -33 of the initiator region and the putative SP1 protein binding site of MxA gene promoter, respectively. A deleted Cytidylic acid was found at nucleotide position -41 which was one nucleotide downstream of the ISRE1 region. G/T and A/C allelic single nucleotide polymorphisms (SNPs) were found at nucleotide positions -89 and -124, respectively. A RFLP (restriction fragment length polymorphism) assay was used for identifying the type of nucleotide or zygote of these two sites. The result showed that C/C and G/G homozygotes exhibited high correlation to each other (P＜ 0.05). Furthermore, among 30 individuals two SNPs showed a high linkage. 29 of 30 individuals had C at nucleotide position -124 and 28 of 30 individuals had C at nucleotide position -124 as well as G at nucleotide position -89, respectively. The linkage reached 96.6% (28/29). 11 of 30 individuals had T at nucleotide position -89 and 9 of 30 individuals had T at nucleotide position -89 as well as A at nucleotide position -124, respectively. The linkage reached 81.8% (9/11).

The human MxA protein is an interferon-inducible protein that confers resistance to influenza virus and other RNA viruses. MxA proteins are synthesized in response to stimulation by type I (α⁄β) interferon in specific organ. Gene expression suggested that at least a region of nucleotide sequence in the MxA gene promoter contains ISRE (interferon stimulated response element). The nucleotide sequences of MxA gene promoters of 30 randomly selected individuals of Southern Min group in southern Taiwan were analyzed. There was no apparent TATA box or CAAT box but a GC-rich region in the MxA gene promoter. Identity of nucleotide sequence of MxA gene promoter from Southern Min group of southern Taiwanese was 98% in comparison with that of NCBI (National Center for Biotechnology Information) GenBank data library. The result demonstrated that a Guanylic acid and a Cytidylic acid were inserted between nucleotide positions -2 and -3 as well as -32 and -33 of the initiator region and the putative SP1 protein binding site of MxA gene promoter, respectively. A deleted Cytidylic acid was found at nucleotide position -41 which was one nucleotide downstream of the ISRE1 region. G/T and A/C allelic single nucleotide polymorphisms (SNPs) were found at nucleotide positions -89 and -124, respectively. A RFLP (restriction fragment length polymorphism) assay was used for identifying the type of nucleotide or zygote of these two sites. The result showed that C/C and G/G homozygotes exhibited high correlation to each other (P＜ 0.05). Furthermore, among 30 individuals two SNPs showed a high linkage. 29 of 30 individuals had C at nucleotide position -124 and 28 of 30 individuals had C at nucleotide position -124 as well as G at nucleotide position -89, respectively. The linkage reached 96.6% (28/29). 11 of 30 individuals had T at nucleotide position -89 and 9 of 30 individuals had T at nucleotide position -89 as well as A at nucleotide position -124, respectively. The linkage reached 81.8% (9/11).