以串聯式液相層析質譜儀同時分析尿液中之麻醉性止痛劑

A Screening Method for Simultaneous Detection of Narcotics in Urine by Liquid Chromatography/Tandem Mass Spectrometry

10.6695/AUES.201005_99.0078

朱文慶(Wen-Ching Chu)；曹立妍(Li-Yan Tsao)；許倍瑄(Bei-Hsuan Hsu)；林淑芬(Shu-Fen Lin)；張簡國平(Kuo-Pin Chang Jan)；許美智(Mei-Tze Hsu)

電噴灑游離法 ； 偵測極限 ； 運動禁藥 ； electrospray ionization ； limit of detection ； doping

大專體育學術專刊

99年度（2010 / 05 / 01）

595 - 601

繁體中文

本文主要為建立分析方法以同時檢測尿液中之13種運動禁藥-麻醉性止痛劑及其代謝物，包括：丁基原啡因（buprenorphine）及其代謝物norbuprenorphine、吩坦尼（fentanyl）及其代謝物 norfentanyl、羥二氫嗎啡（hydromorphone）、美沙冬（methadone）及其代謝物2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine、羥二氫可待因酮（oxycodone）及其代謝物noroxycodone、羥二氫嗎啡酮（oxymorphone）、速賜康（pentazocine）及配西汀（pethidine）及其代謝物 norpethidine。尿液檢體水解後使用單一固相萃取管柱進行萃取，收集混合沖提液，注入串聯式液相層析質譜儀（liquid chromatography / tandem mass spectrometry）以電噴灑游離法（electrospray ionization）之正電模式分析，並採用多重反應監控模式（multiple reaction monitoring）收集分析結果。所有藥物在相對滯留時間（CV%≦0.76）與特徵離子相對反應強度（CV%≦7.09）皆具有極佳的再現性。偵測極限為 2.5～50 ng/mL。所有藥物的偵測極限皆符合世界反運動禁藥組織（World Anti-Doping Agency）所規定的最低要求執行極限。

This paper presents a screening method for simultaneous detection 13 narcotics and their metabolites in urine. These substances included buprenorphine and its metabolite norbuprenorphine, fentanyl its metabolite norfentanyl, hydromorphone, methadone its metabolite 2-ethylidene-1, 5-dimethyl-3, 3-diphenylpyrrolidine, oxycodone its metabolite noroxycodone, oxymorphone, pentazocine, pethidine its metabolite norpethidine. Urine samples were hydrolyzed and extracted by single solid phase extraction cartridge. Eluent was collected and then assayed by LCMS/MS in positive ionization mode using an electrospray ionization and multiple reaction monitoring as the acquisition mode. All compound showed good reproducibility in terms of both the relative retention times (CV%≦0.76) and the relative abundances of the diagnostic transitions (CV%≦7.09). The limit of detection were in the range of 2.5~50 ng/mL, thus satisfying the minimum required performance limits set by the World Anti-Doping Agency.