台灣溫室作業環境生物氣膠分佈特性

The Characteristics of Bacteria and Fungi Bioaerosols Distribution in Taiwan Greenhouses

楊心豪(Shin-Hao Yang)；黃筱茜(Hsiao-Chien Huang)；洪粕宸(Po-Chen Hung)；莊啟佑(Chi-Yu Chuang)；方煒(Wei Fang)

溫室作業環境 ； 生物氣膠 ； 細菌 ； 真菌 ； 分佈 ； Bioaerosols ； Greenhouse ； Bacteria ； Fungi ； Distribution

勞動及職業安全衛生研究季刊

23卷3期（2015 / 09 / 15）

268 - 289

繁體中文

近本研究主要針對溫室作業場所進行空氣中細菌與真菌之調查，以瞭解目前台灣之溫室作業場所中生物性危害之程度。研究中選取3間家溫室作業場所進行採樣分析，細菌與真菌生物氣膠分佈是利用衝擊式（impactor）生物氣膠採樣器進行採樣，採樣分為上午、中午與下午三時段進行採樣。生物氣膠採集後之真菌與細菌樣本則進一步將其純化培養，再予以分子生物鑑定方式進行菌種鑑定。研究結果發現，平均細菌生物氣膠濃度達到最高1,674±296CFU/m^3，真菌生物氣膠最高可達到6,432±981CFU/m^3。菌種鑑定結果顯示，真菌有＂Trichoderma＂ spp.、＂Penicillium＂ spp.、＂Aspergillus＂ spp.、＂Acremonium＂ spp.、＂Meira＂ spp.。細菌則有＂Bacillus＂ spp.、＂Planobacterium＂ spp.、＂Chryseobacterium＂ spp.、＂Jeotgalicoccus＂ spp.、＂Staphylococcus＂ spp. ＂Nocardiopsis＂ spp.、＂Agromyces＂ spp.、＂Micrococcus＂ spp.。

This study aims to investigate the airborne microorganisms and bacteria endotoxins in the greenhouse, for understanding biological hazards in Taiwan greenhouse. Three greenhouses were selected as the testing subjects. The bacteria and fungi bioaerosol distributions were sampling by impactor bioaerosol sampler. The species of bioaerosol samples in the operating workplace were finally purified, cultured, and further identificated by molecular biology. The results showed that in the greenhouses, the highest average bacteria bioaerosol concentrations was in the range of 1674±296 CFU/m^3, and the highest average fungi bioaerosols concentration was about 6,432±981 CFU/m^3. According to the identificated of molecular biology in the greenhouses, bacteria bioaerosols were ＂Bacillus＂ spp., ＂Planobacterium＂ spp., ＂Chryseobacterium＂ spp., ＂Jeotgalicoccus＂ spp., ＂Staphylococcus＂ spp., ＂Nocardiopsis＂ spp., ＂Agromyces＂ spp., and ＂Micrococcus＂ spp.. The fungi bioaerosols were ＂Trichoderma＂ spp., ＂Penicillium＂ spp., ＂Aspergillus＂ spp., ＂Acremonium＂ spp. and ＂Meira＂ spp.

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