题名

電銲金屬燻煙對去氧核醣核酸甲基化影響研究

并列篇名

Effects of Metal Fume Exposure on DNA Methylation

作者

羅敏倩(Min-Chien Lo)；王鐘慶(Chung-Ching Wang)；陳韋良(Wei-Liang Chen)；周雨青(Yu-Ching Chou)；賴錦皇(Ching-Huang La)

关键词

電銲燻煙 ； 微粒 ； 金屬 ； DNA甲基化 ； LINE-1甲基化指標 ； Welding metal fumes ； Urinary metals ； DNA methylation ； LINE-1 methylation biomarker

期刊名称

勞動及職業安全衛生研究季刊

卷期/出版年月

30卷1期（2022 / 03 / 20）

页次

74 - 88

内容语文

繁體中文
中文摘要

本研究之目的主要是探討造船業男性電銲作業人員暴露於電銲之金屬燻煙對DNA異常甲基化的影響，採橫斷性研究設計，共招募100名受試者，暴露組73名，對照組行政人員27名；於星期一早上以八小時PM_(2.5)個人式採樣，並在星期二早上進行健康檢查，收集尿液以感應耦合電漿質譜儀（ICP-MS）分析其尿中金屬濃度；並採集血液樣本，收集DNA後進行亞硫酸鹽轉化及聚合酶連鎖反應後以Mass detection進行甲基化分析；使用問卷收集個人基本資料、生活習慣和工作史。在多變項線性迴歸分析中，校正年齡、工作組別、吸菸習慣和二手菸暴露後，PM_(2.5)濃度與LINE-1 CpG8+9甲基化百分比呈顯著正相關（β=0.311，95%CI 0.000-0.015，p=0.042），尿中鉻濃度與LINE-1 CpG8+9甲基化百分比呈顯著負相關（β=-0.313，95%CI -0.014-0.000，p=0.049）。本研究結論為PM_(2.5)與CpG8+CpG9高甲基化有關；尿中鉻濃度為LINE-1 CpG8+9低甲基化的危險因子。
英文摘要

This study is mainly to explore the relationship between DNA methylation and exposure to welding metal fumes. A total of 100 participants were recruited. Subjects were requested to wear a personal PM_(2.5) sampler on Monday morning for consecutive 8-hour working duration. Urine and fasting blood samples were collected on the next day morning. A questionnaire was adopted to collect personal information, lifestyle and working conditions. Linear regression revealed PM_(2.5) level was significantly and positively associated with LINE-1 CpG8+9 methylation percentage after adjusting for age, working group, current smoking habit, and secondhand smoke exposure. Urinary chromium concentration was significantly and negatively associated with the percentage of methylation of LINE-1 CpG8+9 after adjusted for age, working group, current smoking habit, and secondhand smoke exposure. Logistic regression indicated that odds ratio of low percentage for methylation of LINE-1 CpG8+9 was 2.337 or 3.144 with urinary chromium or urinary iron concentration after adjusted urinary creatinine, age, working group, current smoking habits and secondhand smoke exposure, respectively. To sum up, urinary chromium is significantly associated with hypomethylation of LINE-1.
主题分类 醫藥衛生 > 預防保健與衛生學
醫藥衛生 > 社會醫學
工程學 > 市政與環境工程
参考文献
  1. 楊秀宜,陳家揚,李聯雄,張靜文(2012)。焊接燻煙健康危害新視界。勞工安全衛生研究季刊，20(3)，383-93。
    連結：
  2. Andrea Baccarelli, A,Wright, RO,Bollati, V,Tarantini, L,Litonjua, AA,Suh, HH(2009).Rapid DNA methylation changes after exposure to traffic particles.American Journal of Respiratory and Critical Care Medicine,179,572-578.
  3. Baba, Y,Yagi, T,Sawayama, H,Hiyoshi, Y,Ishimoto, T,Iwatsuki, M(2018).Long Interspersed Element-1 Methylation Level as a Prognostic Biomarker in Gastrointestinal Cancers.Digestion,97,26-30.
  4. Baccarelli, A,Bollati, V(2009).Epigenetics and environmental chemicals.Current Opinion in Pediatrics,21,243-251.
  5. Baccarelli, A,Rienstra, M,Benjamin, EJ(2010).Cardiovascular epigenetics: basic concepts and results from animal and human studies.CirculationCardiovascular Genetics,3,567-573.
  6. Baccarelli, A,Tarantini, L,Wright, RO,Valentina Bollati, V,Litonjua, AA,Zanobetti, A(2010).Repetitive element DNA methylation and circulating endothelial and inflammation markers in the VA normative aging study.Epigenetics,5,222-228.
  7. Bird, A.(2002).DNA methylation patterns and epigenetic memory.Genes & Development,16,6-21.
  8. Cash, HL,McGarvey, ST,Houseman, EA,Marsit, CJ,Hawley, NL,Lambert-Messerlian, GM(2011).Cardiovascular disease risk factors and DNA methylation at the LINE-1 repeat region in peripheral blood from Samoan Islanders.Epigenetics,6,1257-1264.
  9. Cavallari, JM,Eisen, EA,Fang, SC,Schwartz, J,Hauser, R,Robert, F,Herrick, RF(2008).PM2.5 metal exposures and nocturnal heart rate variability: a panel study of boilermaker construction workers.Environmental Health,7,36.
  10. Christiani, DC(2009).Association between fine particulate matter and oxidative DNA damage may be modified in individuals with hypertension.Journal of Occupational and Environmental Medicine,51,1158-1166.
  11. Davis, CD,Uthus, EO(2004).DNA Methylation, Cancer Susceptibility, and Nutrient Interactions.Experimental Biology and Medicine,229,988-995.
  12. Evans, PJ,Whiteman, M,Tredger, JM,Halliwell, B(1997).Antioxidant Properties of S-Adenosyl-l-Methionine: A Proposed Addition to Organ Storage Fluids.Free Radical Biology and Medicine,23,1002-1008.
  13. Fan, T,Fang, SC,Cavallari, JM,Barnett, IJ,Wang, Z,Su, L(2014).Heart rate variability and DNA methylation levels are altered after short-term metal fume exposure among occupational welders: a repeated-measures panel study.BMC public health,14,1279.
  14. Fiskin, K,Ozkan, A,Ayhan, AG(2006).Investigation of protective effects of selenium and vitamin e against DNA oxidation, membrane damage and alteration of comt metabolism in smoke-exposed male mice (Mus Musculus, Balb/c).Acta Biologica Hungarica,57,403-413.
  15. Forman, SA(1988).US Navy shipyard occupational medicine through World War II.J Occupational Medicine,30,28-32.
  16. Franchini, DM,Schmitz, KM,Petersen-Mahrt, SK(2012).5-Methylcytosine DNA demethylation: more than losing a methyl group.Annual Review of Genetics,46,419-441.
  17. Han, BC,Liu, IJ,Chuang, HC,Pan, CH,Chuang, KJ(2016).Effect of welding fume on heart rate variability among workers with respirators in a shipyard.Scientific Reports,6,34158.
  18. Jones, PA,Baylin, SB(2002).The fundamental role of epigenetic events in cancer.Nature Reviews Genetics,3,415.
  19. Kile, ML,Fang, S,Baccarelli, AA,Tarantini, L,Cavallari, J,Christiani, DC(2013).A panel study of occupational exposure to fine particulate matter and changes in DNA methylation over a single workday and years worked in boilermaker welders.Environmental Health,12,47.
  20. Kim, J,Chen, J,Boyce, P,Christiani, D(2005).Exposure to welding fumes is associated with acute systemic inflammatory responses.Occupational and Environmental Medicine,62,157-163.
  21. Knaapen, AM,Borm, PJA,Albrecht, CA,Schins, RPF(2004).Inhaled particles and Lung cancer. Part A: Mechanisms.International Journal of Cancer,109,799-809.
  22. Leso, V,Vetrani, I,Volpe, ID,Nocera, C,Ivo Iavicoli, I(2019).Welding Fume Exposure and Epigenetic Alterations: A Systematic Review.International journal of environmental research ic health,16,1745.
  23. Ray, PD,Yosim, A,Fry, RC(2014).Incorporating epigenetic data into the risk assessment process for the toxic metals arsenic, cadmium, chromium, lead, and mercury: strategies and challenges.Frontiers in Genetics,5,201.
  24. Richardson, B(2003).Impact of aging on DNA methylation.Ageing Research Reviews,2,245-261.
  25. Robertson, KD(2005).DNA methylation and human disease.Nature Reviews Genetics,6,597.
  26. Russo, AL,Thiagalingam, A,Pan, H,Califano, J,Cheng, KH,Ponte, JF(2005).Differential DNA Hypermethylation of Critical Genes Mediates the Stage-Specific Tobacco Smoke-Induced Neoplastic Progression of Lung Cancer.Clinical Cancer Research,11,2466-2470.
  27. Sani, A,Abdullahi, IL(2019).Effects of welding fumes on haematological parameters of male albino rats (Rattus norvegicus).Biochemistry and biophysics reports,19,100651.
  28. Shen, XM,Dryhurst, G(1998).Iron- and Manganese-Catalyzed Autoxidation of Dopamine in the Presence of l-Cysteine: Possible Insights into Iron- and Manganese-Mediated Dopaminergic Neurotoxicity.Chemical Research in Toxicology,11,824-837.
  29. Shoeb, M.,Mustafa, GM,Kodali, VK,Smith, K,Roach, KA,Boyce, G(2020).A possible relationship between telomere length and markers of neurodegeneration in rat brain after welding fume inhalation exposure.Environmental research,180,108900.
  30. Valinluck, V,Tsai, HH,Rogstad, DK,Burdzy, A,Bird, A,Sowers, LC(2004).Oxidative damage to methyl-CpG sequences inhibits the binding of the methyl-CpG binding domain (MBD) of methyl-CpG binding protein 2 (MeCP2).Nucleic acids research,32,4100-4108.
  31. Valko, M,Morris, H,Cronin, MT(2005).Metals, toxicity and oxidative stress.Current Medicinal Chemistry,12,1161-1208.
  32. Wang, T,Garcia, JG,Zhang, W(2012).Epigenetic Regulation in Particulate Matter-Mediated Cardiopulmonary Toxicities: A Systems Biology Perspective.Current pharmacogenomics and personalized medicine,10,314-321.
  33. Wong, JYY,Bryan, A,Bassig, BA,Seow, WJ,Hu, W,Ji, BT(2017).Lung cancer risk in welders and foundry workers with a history of heavy smoking in the USA: The National Lung Screening Trial.Occupational and Environmental Medicine,74,440-448.
  34. Wu, H,Zhang, Y(2014).Reversing DNA methylation: mechanisms, genomics, and biological functions.Cell,156,45-68.
  35. Yang, AS,Estécio, MRH,Doshi, K,Kondo, Y,Tajara, EH,Issa, JPJ(2004).A simple method for estimating global DNA methylation using bisulfite PCR of repetitive DNA elements.Nucleic Acids Research,32,e38.
  36. 陳秋蓉(2002).造船廠勞工健康情形調查.行政院勞工委員會勞工安全衛生研究所.
  37. 豐技生物科技股份有限公司(2017)。豐技生物科技股份有限公司，EpiTYPER DNA 甲基化分析原理；2017。
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