毛細電泳－質譜儀

Capillary Electrophoresis-Mass Spectrometry

10.6623/chem.1995046

李丁在(D. T. Li)；何國榮(G. R. Her)

化學

53卷4期（1995 / 12 / 01）

438 - 445

繁體中文

本文介紹五種以電灑法(ESI) 和電灑－化學離子化法(ESI-CI) 所構成的毛細電泳－質譜儀(CE-MS)界面。毛細管末稍金屬化界面是最初設計的界面；鞘流界面和液體結合界面的使用最為廣泛；無鞘流界面有最高的靈敏度；電灑-化學離子化法界面允許非揮發性鹽類的使用與中性分子的分析。在技術應用方面，藉由縮小毛細管管徑和前濃縮的技術可提高靈敏度和降低偵測極限。此外，低電滲透流(EOF)使得高濃度電解質不會干擾質譜儀的訊號，然而當使用的是鞘流界面，鞘溶液的組成可能會嚴重影響毛細電泳的分離效果。最後舉一實例說明毛細電泳－電灑法－傅氏轉換質譜儀(CE-ESI-FTMS)分析細胞內蛋白質的可行性。

Capillary electrophoresis-mass spectrometry (CE-MS) interfaces based upon electrospray ionization (ESI) and electrospray-chemical ionization (ESI-CI) are illustrated. The metallized capillary terminus interface is the first design for coupling CE with MS. The sheath flow interface and the liquid junction interface are the two most often used interfaces. The sheathless interface offers the greatest sensitivity. The electrospray-chemical ionization interface allows the use of nonvolatile buffers and the analysis of neutral compounds. Small diameter fused silica capillaries and preconcentration techniques have been used for improvement of detection limits. The zero electroosmotic flow (EOF) in wall-coated fused silica capillaries enables the use of high concentration background electrolytes without interfering the mass spectrometric signal. However, the CE separation may be affected by the sheath liquid if a sheath flow interface is used. One example is used to demonstrate the feasibility of CE-ESI-FTMS (fourier transform mass spectrometry) in the analysis of cellular proteins directly from cells.