以黃酮結構探討藍花楹抗氧化活性

A Study of the Structural Analysis on Antioxidant Properties of Flavonoids Extracted from Jacaranda Acutifolia

10.6763/IJTCMA.202005_4(1).0003

陳玉芳(Chen, Yu-Fang)

抗氧化活性 ； 藍花楹 ； 黃酮化合物 ； Antioxidant activity ； Jacaranda acutifolia ； Flavonoid compounds

中醫本草芳香療法期刊

4卷1期（2020 / 05 / 15）

49 - 64

繁體中文

為探討藍花楹萃取物作為抗氧化化妝品的可行性，本研究先以藍花楹花部丙酮萃取物進行抗氧化活性評估，經實驗結果顯示其萃取物乙酸乙酯層具有最明顯的清除DPPH自由基與螯合亞鐵的能力。本研究續將上述乙酸乙酯層與正丁醇層進行活性導向成分分離與鑑定，獲得五種黃酮化合物，進一步比較黃酮化合物之結構與抗氧化活性間的關係。實驗結果顯示此五種黃酮化合物清除DPPH自由基IC_(50)值範圍介於0.18至0.82mg/mL之間。

To evaluate the feasibility of using Jacaranda acutifolia extract as an antioxidant ingredient for cosmetics, a series of antioxidant activity assays were performed on the acetone extracts from its flower. The results indicated that the ethyl acetate soluble fraction (JAFE) of Jacaranda acutifolia flower extracts exhibited the highest DPPH radical scavenging and ferric ion reducing activities. The ethyl acetate soluble fraction (JAFE) and butyl alcohol soluble fraction (JAFB) of the extracts were further separated for constituent identification, and five flavonoid compounds were obtained. The structural related antioxidant properties of above flavonoid compounds were examined. Test results revealed that IC_(50) values of DPPH free radical scavenging capability for these five compounds were ranged from 0.18 to 0.82 mg/mL.

1. 洪千青,翁義銘,余哲仁,吳孟珊,王璧娟(2019)。超臨界流體區影養米糠萃取液抗氧化成分的分布。台灣食品業化學與食品科學，57(2)，79-88。
   連結：
2. Benavente, O.,Castillo, G. J.,Lorente, J.,Ortuno, A.(2000).Anti-oxidation activity of phenolics extracted from Olea europaea L.Leaves.Food Chem,68,457-462.
3. Binutu, O. A.,Lajubutu, B.A.(1994).Antimicrobial potentials of some plant species of the bignoniaceae family.African Medicine Medical Sci,23,269-412.
4. Braca, A.,Morelli, I.,Simone, F. D.,Tome, F.,Tommasi, N. D.(2003).Antioxidant and free radical scavenging activity of flavonol glycosides from different Aconitum species.J. Ethnopharmacol,86,63-67.
5. Catherine, A. R.,Nicholas, J. M.(1996).Structure antioxidant activity relationships of flavonoids and phenolic acid.Free Radical Biology ＆ Medicine,20(7),933-956.
6. Dorman, HJD.,Kosar, M.,Kahlos, K.,Holm, Y.,Hiltunen, R.(2003).Antioxidant properties and composition of aqueous extracts from Mentha species hybrids,varieties and cultivars.J.Agric Food. Chem,51,4563-4569.
7. George, K.B.,Herbert, M.S.(1999).Polyphenol tannic acid inhibits hydroxy radical formation from Fenton reaction by complexing ferrous ions.Biochimica et Biophysica Acta.,1472,142-152.
8. Joshi, K. C. J.,Bansal, R. K.,Singh, P.,Singh, G.(1975).Components of the stem barks of Phyllarthron comorense and Jacaranda mimosaefolia and roots of Desmodium pulchellum.Indian J. Chem.,13(8),869-870.
9. Michael, J.(2002).Investigation of the release of iron from ferritin by naturally occurring antioxidants.J.Inorg Biochem,90,18-21.
10. Michikatsu, S.V.(1996).Differences in the phenolic content and superoxide radical scavenging potential of wines from different sources.J. Agric. Food Chem,44,37-41.
11. Ogura, M.,Cordell, G.. A.,Farnsworth, N. R.(1976).Potential anticancer agents (III) Jacaranone, a novel phytoquinoid from Jacaranda caucana.Lloydia,39(4),255-257.
12. Ogura, M.,Cordell, G.. A.,Farnsworth, N. R.(1977).Potential anticancer agents (IV) Constituents of Jacaranda caucana Pittier.Lloydia,40,157-168.
13. Sato, M.,Ramarathnam, N,Suzuki, Y.,Ohkubo, T.,Takeuchi, M.(1996).Varietal differences in the phenolic content and superoxide radical scavenging potential of wines from different source.J. Agric. Food Chem,44,37-41.
14. Weniger, B.,Robledo, S.,Arango, G. J.(2001).Antiprotozoal activities of Colombian plants.J. Ethnopharmaco,78,193-200.
15. 李靜芳,林炳宏,王申豪,吳思敬(2018)。市售常見中草藥植物抗氧化活性之評估。嘉大農林學報，15(2)，35-49。
16. 周欣初(2003).天然中草藥化妝品.渡假出版社有限公司.
17. 徐嘉琪 (民 104)，皮膚老化和抗氧化物的關係，取自https://news.everydayhealth.com.tw/2015/05/09/15175-%E7%9A%AE%E8%86%9A%E8%80%81%E5%8C%96%E5%92%8C%E6%8A%97%E6%B0%A7%E5%8C%96%E7%89%A9%E7%9A%84%E9%97%9C%E4%BF%82
18. 鄧凱云,楊筱姿,陳怡宏,林欣榜,蔡孟貞(2011)。國產柑橘類果皮製成陳皮之多甲基類黃酮含量及抗氧化活性研究。台灣食品業化學與食品科學，49(6)，338-345。