Feasibility Study of Green Tea Extract as a Relief of Urinary Tract Inflammation

探討綠茶提取物作為緩解尿道炎症之可行性研究

10.6763/IJTCMA.202011_4(2).0003

林文宏(Lin, Wen-Hung)；林彥伶(Lin, Yen-Ling)；劉育奇(Liu, Yu-Chi)；鄭麗秋(Cheng, Li-Chiu)

green tea extract ； tea polyphenols ； urinary tract infection ； anti-inflammatory ； 綠茶提取物 ； 茶多酚 ； 泌尿道感染 ； 抗發炎

中醫本草芳香療法期刊

4卷2期（2020 / 11 / 15）

47 - 61

英文

For many years, polyphenols are rich in antioxidant substances and has been promoted as a type of food which has been used to alleviate urinary tract infection (UTI). Since it has been considered as one of the non-antibiotic preventive choices, and be mostly used by the public. Studies have found that women have a 50% UTI risk in their lifetime, and even up to 20-30% experience subsequent UTI recurrence. Therefore, the purpose of this study is to use green tea extract to inhibit nitric oxide (NO), cytokine interleukin 6 (IL-6), and tumor necrosis factor-α (tumor necrosis factor ; TNF-α), research on alleviating urinary tract inflammation factors, and conduct research on alleviating inflammation factors in the urinary tract. The results have shown that the green tea extracts can significantly inhibit the inflammatory NO response which has been stimulated by bacterial lipopolysaccharide (LPS). The low dose (2.5% in cell culture medium), medium dose (5% in cell culture medium), and high dose (10% in cell culture solution) would often result in NO inducted inhibition of 5.5%, 16.8%, and 27.6% respectively. The extract of Green Tea can significantly inhibit the inflammatory IL-6 response which is stimulated by bacterial lipopolysaccharide. The low dose (2.5% in cell culture medium), medium dose (5% in cell culture medium), and high dose (10% in cell culture solution) would result in IL-6 inducted inhibition of 28.7%, 42.3%, and 50.3%, respectively. Yet, there was no inhibitory response to TNF-α within the same time. These extractions of Green Tea do not contain cytotoxicity within the tested cells. Nevertheless, Green tea extract has anti-inflammatory effects. However, there is no direct evidence to prove the test results that can reduce inflammation of the urethra. Therefore, the results of this cell test could provide to relevant experts and scholars for reference for direct evidence research. This is worthy of further research and discussion in the future. At the same time, more clinical trials and higher-quality research are needed.

多年來富含抗氧化的多酚類物質，一直被提倡用於緩解尿道感染（urinary tract infection, UTI）所使用的食品，因其是非抗生素預防性選擇之一的品項，多為社會大眾所鍾愛。研究發現女性在其一生中有50％的UTI風險，甚至有高達20－30％經歷了隨後的UTI復發現象。因此，本研究的目的是以綠茶提取物進行緩解泌尿道發炎因子之研究，以藉由抑制一氧化氮（nitric oxide, NO）、介白素6（cytokine interleukin 6; IL-6）、腫瘤壞死因子-α（tumor necrosis factor; TNF-α），提出對三種抗發炎之研究，以減輕泌尿道感染的風險，並為這些疾病的醫療保健提供了建議。結果表明，綠茶提取物顯著抑制由細菌脂多醣（LPS）刺激的炎症性NO反應。在低劑量（在細胞培養基中為2.5％），中等劑量（在細胞培養基中為5％）和高劑量（在細胞培養基中為10％）導致NO誘導的抑制作用分別為5.5％，16.8％和27.6％。綠茶提取物顯著抑制細菌性脂多醣刺激炎症性IL-6反應。在低劑量（在細胞培養基中為2.5％），中等劑量（在細胞培養基中為5％）和高劑量（在細胞培養基中為10％）導致IL-6誘導的抑制作用分別為28.7％，42.3％和50.3％。然而，在同一時間沒有對TNF-α的產生抑制反應。同時，這些綠茶提取物在測試的細胞中不具細胞毒性。雖然如此，綠茶提取物雖然具有抗發炎作用。但是，沒有直接證據證明，具有可以減輕尿道炎症的試驗結果。因此，以本次的細胞試驗結果，提供給相關的專家學者，再進行直接證據研究的參考。這是值得在以後進一步的研究和討論，同時還需要通過更多的臨床試驗和更多更高質量的研究。

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