Traditional Chinese medicine herbal extracts of Cibotium barometz, Gentiana scabra, Dioscorea batatas, Cassia tora, and Taxillus chinensis inhibit SARS-CoV replication

Chih-Chun Wen；Lie-Fen Shyur；Jia-Tsrong Jan；Po-Huang Liang；Chih-Jung Kuo；Palanisamy Arulselvan；Jin-Bin Wu；Sheng-Chu Kuo；Ning-Sun Yang

Severe acute respiratory syndrome (SARS) ； Traditional Chinese medicine (TCM) ； Cytopathogenic effect (CPE) ； SARS 3CL protease ； Cibotium barometz

Journal of Traditional and Complementary Medicine

1卷1期（2011 / 10 / 01）

41 - 50

英文

Development of anti-severe acute respiratory syndrome associated coronavirus (SARS-CoV) agents is pivotal to prevent the reemergence of the life-threatening disease, SARS. In this study, more than 200 extracts from Chinese medicinal herbs were evaluated for anti-SARS-CoV activities using a cell-based assay that measured SARS-CoV-induced cytopathogenic effect (CPE) in vitro on Vero E6 cells. Six herbal extracts, one each from Gentianae Radix (龍膽 lóng dǎn; the dried rhizome of Gentiana scabra ), Dioscoreae Rhizoma (山藥 shān yào; the tuber of Dioscorea batatas ), Cassiae Semen (決明子 jué míng zǐ; the dried seed of Cassia tora ) and Loranthi Ramus (桑寄生 sāng jì shēng; the dried stem, with leaf of Taxillus chinensis ) (designated as GSH, DBM, CTH and TCH, respectively), and two from Rhizoma Cibotii (狗脊 gǒu jǐ; the dried rhizome of Cibotium barometz ) (designated as CBE and CBM), were found to be potent inhibitors of SARS-CoV at concentrations between 25 and 200 μg/ml. The concentrations of the six extracts needed to inhibit 50% of Vero E6 cell proliferation (CC_(50)) and 50% of viral replication (EC_(50)) were determined. The resulting selective index values (SI = CC_(50)/EC_(50)) of the most effective extracts CBE, GSH, DBM, CTH and TCH were ＞ 59.4, ＞ 57.5, ＞ 62.1, ＞ 59.4, and ＞ 92.9, respectively. Among these extracts, CBM and DBM also showed significant inhibition of SARS-CoV 3CL protease activity with IC_(50) values of 39 μg/ml and 44 μg/ml, respectively. Our findings suggest that these six herbal extracts may have potential as candidates for future development of anti-SARS therapeutics.

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