Antiviral Decoction of Isatidis Radix (板藍根 bǎn lán gēn) Inhibited Influenza Virus Adsorption on MDCK Cells by Cytoprotective Activity

Lijing Ke；Teng Wen；Jeremy P Bradshaw；Jianwu Zhou；Pingfan Rao

Isatidis Radix (板藍根 bǎn lán gēn) ； Antiviral effects ； Cell binding ； Influenza virus ； Cell protection

Journal of Traditional and Complementary Medicine

2卷1期（2012 / 01 / 01）

47 - 51

英文

The aim of this study is to elucidate how the Isatidis Radix (板藍根 bǎn lán gēn) tonic, as an aqueous mixture of hundreds of compositions, interrupts the infection of influenza viruses to their host cells. The efficacy of the tonic was evaluated and expressed as cell proliferation rate and plaque reduction rate in Madin-Darby Canine Kidney (MDCK) cells, against 3 strains of influenza A and B viruses. This boiling water (at 100℃) extract of Isatidis Radix (RIE) showed antiviral activity against influenza virus A and B. The concentration for 50% inhibition of influenza virus A replication (IC_(50)) in MDCK cell was 12.6 mg/mL with a therapeutic index ＞8. When cells were incubated with RIE prior to virus adsorption, the numbers of viable cell were at least doubled compared to the numbers of virus control, RIE incubation after virus adsorption and RIE incubation with virus prior to adsorption, in both influenza virus A and B. Moreover, much less virus particles were spotted by scanning electron microscope (SEM) in the RIE pre-treated cells than the cells without RIE treatment. These results indicate the antiviral activity of RIE is mainly attributed to its host cell protection effect but not actions on virus or post-virus-adsorption interruption. Cell, but not virus, is more likely to be the action target of RIE.

1. Burleson, F.G. (Ed.)(1992).Virology A Laboratory Manual.California:Academic Press, Inc..
2. Chen, Z.W.,Liu, S.T.,Cai, C.P.,Rao, P.F.,Ke, L.J.(2006).Mechanism study of anti-influenza effects of radix Isatidis water extract by red blood cells capillary electrophoresis.China Journal of Chinese Material Medica,31,1715-1719.
3. China Pharmacopeia Committee(2005).China Pharmacopeia.Chemical Industry Press.
4. Gaush, C.R.,Smith, T.F.(1968).Replication and plaque assay of influenza virus in an established line of canine kidney cells.Applied Microbiology,16,588-594.
5. Kawaoka Y. (Ed.)(2006).Influenza virology: current topics.England:Caister Academic Press.
6. Ke, L.J.,Zhou, J.W.,Lu, W.,Gao, G.Z.,Rao, P.F.(2011).The power of soups: super-hero or team-work?.Trends in Food Science and Technology,22,492-497.
7. Levi, R.,Beeor-Tzahar, T.,Arnon, R.(1995).Microculture virus titration-a simple colourimetric assay for influenza virus titration.Journal of Virological Methods,52,55-64.
8. Lin, C.W.,Tsai, F.J.,Tsai, C.H.,Lai, C.C.,Wan, L.,Ho, T.Y.,Hsieh, C.C.,Chao, P.L.(2005).Anti-SARS coronavirus 3C-like protease effects of Isatis indigotica root and Plant-derived Phenolic Compounds.Antiviral Research,68,36-42.
9. Lu, W.H.,Deng, W.H.,Liu, S.T.,Chen, T.B.,Rao, P.F.(2003).Capillary electrophoresis of erythrocytes.Analytical Biochemistry,314,194-198.
10. Moscona, A.(2005).Neuraminidase Inhibitors for Influenza.New England Journal of Medicine,353,1363-1373.
11. Nagai, T.,Yang, J.W.,Sakurai, M.,Yamada, H.(1998).Anti-influenza viral glycoprotein from the roots of Isatis tinctoria L.Antiviral Research,37,88.
12. Pinto, L.H.,Lamb, R.A.(2007).The M2 Proton Channels of Influenza A and B Viruses.Journal of Biological Chemistry,281,8997-9000.
13. Reading, S.A.,Dimmock, N.J.(2007).Neutralization of animal virus infectivity by antibody.Archives of Virology,152,1047-1059.
14. Reed, L., Muench, H., 1938. A simple method of estimating fifty percent endpoints. The American Journal of Hygiene 27, 493-497.
15. Schmidtke, M.,Schnittler, U.,Jahn, B.,Dahse, H.,Stelzner, A.(2001).A rapid assay for evaluation of antiviral activity against coxsackie virus B3, Influenza virus A, and herpes simplex virus type 1.Journal of Virological Methods,95,133-143.
16. Tashiro, M.,Rott, R.(1996).The role of proteolytic cleavage of viral glycoproteins in the pathogenesis of influenza virus infections.Seminars in Virology,7,237-243.
17. van de Loosdrecht, A.A.,Beelen, R.H.J.,Ossenkoppele, G.J.,Broekhoven, M.G.,Langenhuijsen, M.M.A.C.(1994).A tetrazolium-based colorimetric MTT assay to quantitate human monocyte mediated cytotoxicity against leukemic cells from cell lines and patients with acute myeloid leukemia.Journal of Immunological Methods,174,311-320.
18. Watanabe, S.,Watanabe, T.,Noda, T.,Takada, A.,Feldmann, H.,Jasenosky, L.D.,Kawaoka, Y.(2004).Production of novel Ebola virus-like particles from cDNAs: an alternative to Ebola virus generation by reverse genetics.Journal of Virology,78,999-1005.

1. Pingfan Rao,Lijing Ke,Jingke Guo,Jie Liu,Jianwu Zhou,Huiqin Wang,Guanzhen Gao,Dai Lin(2017).Boiling-induced nanoparticles and their constitutive proteins from Isatis indigotica Fort. root decoction: Purification and identification.Journal of Traditional and Complementary Medicine,7(2),178-187.