Naturally Occurring Cytotoxic [3' → 8＂]-Biflavonoids from Podocarpus nakaii

Pen-Ho Yeh；Yun-Dar Shieh；Li-Chun Hsu；Li-Ming Yang Kuo；Jhih-Hu Lin；Chia-Ching Liaw；Yao-Haur Kuo

Podocarpus nakaii ； [3'→8＂]-Biflavonoids ； Cytotoxicity ； Apoptosis ； Topoisomerase I

Journal of Traditional and Complementary Medicine

2卷3期（2012 / 07 / 01）

220 - 226

英文

Bioassay-guided fractionation of the EtOH extract of the dried twigs of Podocarpus nakaii Hayata (Podocarpaceae), endemic plant in Taiwan has resulted in isolation of four [3'→8＂]-biflavonoid derivatives, amenotoflavone (AF), podocarpusflavone-A (PF), II-4＂,I-7-dimethoxyamentoflavone (DAF), and heveaflavone (HF). Their structures were determined by physical and extensive spectroscopic analyses such as ^1H, ^(13)C, ^1H-^1H COSY, HMQC, and HMBC, as well as comparison with literature values. Compounds PF and DAF showed significant inhibitions against DLD, KB, MCF-7, HEp-2 tumor cell lines (ED_(50) ca . 4.56-16.24 μg/mL) and induced cell apoptosis in MCF-7 via mainly sub-G_1/S phase arrest. Furthermore, these compounds exhibited moderate Topoisomerase I inhibitory activity.

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